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It was shown that bidistilled modified water induces a marked decrease in the intensity of intrinsic fluorescence of bovine serum albumin and increases the binding of this protein to the fluorescent probe 1.8 ANS. These effects can be interpreted as a denaturing action of bidistilled modified water on the protein and a change in its conformational state, which is probably caused by changes in the microenvironment of the protein molecule. In addition, a substantial increase in the intrinsic fluorescence of bidistilled modified water, as compared with that of distilled water, was found. 相似文献
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Multiple isoforms of UDP-GalNAc:polypeptide N-acetylgalactosaminyl-
transferase (ppGaNTase) have been cloned and expressed from a variety of
organisms. In general, these isoforms display different patterns of
tissue-specific expression, but exhibit overlapping substrate
specificities, in vitro . A peptide substrate, derived from the sequence of
the V3 loop of the HIV gp120 protein (HIV peptide), has previously been
shown to be glycosylated in vitro exclusively by the ppGaNTase-T3 (Bennett
et al. , 1996). To determine if this isoform- specificity is maintained in
vivo , we have examined the glycosylation of this substrate when it is
expressed as a reporter peptide (rHIV) in a cell background (COS7 cells)
which lacks detectable levels of the ppGaNTase-T3. Glycosylation of rHIV
was greatly increased by coexpression of a recombinant ppGaNTase-T3.
Overexpression of ppGaNTase- T1 yielded only partial glycosylation of the
reporter. We have also determined that the introduction of a proline
residue at the +3 position flanking the potential glycosylation site
eliminated ppGaNTase- T3 selectivity toward rHIV observed both in vivo and
in vitro .
相似文献
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Novikov VV Lisitsyn AS Fesenko EE Aliushev FK Eremin SM Markov IA Ten IuA 《Biofizika》2001,46(2):242-244
It was shown that bidistilled modified water substantially enhances the hydrolysis of the peptide the bovine insulin B-chain. The exposure of the peptide to bidistilled modified water for 20 hours at room temperature leads to an almost complete hydrolysis of its molecule into fragments that differ from the initial molecule in elution time from a column in high-performance liquid chromatography. 相似文献
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Fesenko EE Novoselova EG Ogaĭ VB Agafonova TA Glushkova OV Sinotova OA Aliushev FK Eremin SM Markov IA Ten IuA 《Biofizika》2001,46(2):353-358
The effect of bidistilled modified water obtained by treating bidistilled water with static electric field in the cathode compartment of a diaphragm electrolyzer on the production of tumor necrosis factor and activity of natural killer cells of mice was studied. It was found that bidistilled modified water added to cultured murine macrophages increases the tumor necrosis factor production 2.5-3-fold. A similar degree of stimulation of tumor necrosis factor production was observed after peroral administration of bidistilled modified water for 10-25 days. The activity of natural killer cells under these conditions increased by 150%. The stimulation of these immunity system components responsible for the resistance of the organism to tumors led to a suppression of tumor growth in tumor-bearing mice receiving bidistilled modified water. 相似文献
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