全文获取类型
收费全文 | 5786篇 |
免费 | 531篇 |
国内免费 | 2篇 |
出版年
2023年 | 39篇 |
2022年 | 84篇 |
2021年 | 212篇 |
2020年 | 108篇 |
2019年 | 161篇 |
2018年 | 193篇 |
2017年 | 146篇 |
2016年 | 232篇 |
2015年 | 418篇 |
2014年 | 362篇 |
2013年 | 393篇 |
2012年 | 541篇 |
2011年 | 505篇 |
2010年 | 301篇 |
2009年 | 217篇 |
2008年 | 347篇 |
2007年 | 341篇 |
2006年 | 306篇 |
2005年 | 269篇 |
2004年 | 236篇 |
2003年 | 237篇 |
2002年 | 190篇 |
2001年 | 41篇 |
2000年 | 35篇 |
1999年 | 47篇 |
1998年 | 51篇 |
1997年 | 22篇 |
1996年 | 16篇 |
1995年 | 17篇 |
1994年 | 14篇 |
1993年 | 9篇 |
1992年 | 9篇 |
1991年 | 10篇 |
1990年 | 11篇 |
1988年 | 11篇 |
1986年 | 9篇 |
1985年 | 8篇 |
1984年 | 9篇 |
1983年 | 9篇 |
1981年 | 9篇 |
1980年 | 7篇 |
1978年 | 7篇 |
1977年 | 9篇 |
1976年 | 8篇 |
1975年 | 7篇 |
1974年 | 7篇 |
1973年 | 12篇 |
1972年 | 8篇 |
1971年 | 11篇 |
1962年 | 7篇 |
排序方式: 共有6319条查询结果,搜索用时 15 毫秒
1.
2.
3.
Twelve cases of methanol poisoning are reviewed. The clinical presentation and biochemical features are described and the results of treatment with alkali, ethanol and dialysis reported. The outcome of methanol poisoning appears to be related more to the interval between the time of ingestion and the start of therapy and to the degree of acidosis than to the initial serum methanol level. Therefore, early and aggressive treatment with bicarbonate and ethanol and subsequent institution of hemodialysis are strongly recommended whenever methanol can be detected in the blood, especially when metabolic acidosis of the anion-gap type is present, when mental or visual disturbances are present, or when more than 30 ml of absolute methanol has been consumed. 相似文献
4.
5.
Abstract. Contrary to current assumptions, the reflex blood of two-spot ladybirds, Adalia bipunctata , and seven-spot ladybirds, Coccinella septempunctata , contains haemocyte-like cells. Furthermore, DNA can be extracted and amplified from coccinellid reflex blood, confirming the presence of haemocyte-like cells and demonstrating a nondestructive method of DNA extraction. 相似文献
6.
Thomas Jahns Alexandra Zobel Diethelm Kleiner Heinrich Kaltwasser 《Archives of microbiology》1988,149(5):377-383
Evidence for the existence of an energy-dependent urea permease was found for Alcaligenes eutrophus H16 and Klebsiella pneumoniae M5a1 by studying uptake of 14C-urea. Since intracellular urea was metabolized immediately, uptake did not result in formation of an urea pool. Evidence is based on observations that the in vivo urea uptake and in vitro urease activity differ significantly with respect to kinetic parameters, temperature optimum, pH optimum, response towards inhibitors and regulation. The K
m for urea uptake was 15–20 times lower (38 M and 13 M urea for A. eutrophus and K. pneumoniae, respectively) than the K
m of urease for urea (650 M and 280 M urea), the activity optimum for A. eutrophus was at pH 6.0 and 35°C for the uptake and pH 9.0 and 65°C for urease. Uptake but not urease activity in both organisms strongly decreased upon addition of inhibitors of energy metabolism, while in K. pneumoniae, potent inhibitors of urease (thiourea and hydroxyurea) did not affect the uptake process. Significant differences in the uptake rates were observed during growth with different nitrogen sources (ammonia, nitrate, urea) or in the absence of a nitrogen source; this suggested that a carrier is involved which is subject to nitrogen control. Some evidence for the presence of an energy-dependent uptake of urea was also obtained in Pseudomonas aeruginosa DSM 50071 and Providencia rettgeri DSM 1131, but not in Proteus vulgaris DSM 30118 and Bacillus pasteurii DSM 33.Non-standard abbreviations CCCP
Carbonylcyanide-m-chlorphenylhydrazone
- DCCD
dicyclohexylcarbodiimide
- DNP
2,4-dinitrophenole 相似文献
7.
8.
The 'two-step' model proposed by Jensen and his collaborators for explaining estrogen action conceptualized hormone-free estrogen receptors (ER) to be cytoplasmic, and hormone-filled, transformed ER to be nuclear. Applying monoclonal antibodies which recognized epitopes in ER and formaldehyde-fixed tissues, King et al demonstrated exclusively nuclear staining in target tissues utilizing immunoperoxidase technique. Recently these antibodies have become commercially available enabling other investigators to conduct studies. In this report, using these monoclonal antibodies we have demonstrated that a change in the concentration of formaldehyde alters the staining pattern yielding cytoplasmic instead of nuclear staining in calf uterus, MCF-7 cells, and ER(+) human breast cancer. In addition, neutralization of the antibody activity was not achieved with freshly prepared ER(+) cytosols. Formaldehyde-treated cytosols were essential. These results ought to caution investigators in determining in vivo location of antigens based on the staining pattern obtained in fixed tissues. Furthermore, this effect of formaldehyde on estrogen receptors may be applicable to other steroid hormone receptors. 相似文献
9.
10.
Sean Waters Muhammed Khamis Alexandra von der Decken 《Molecular and cellular biochemistry》1992,109(1):17-24
Atlantic salmon (Salmo salar) were treated with 17- estradiol to induce vitellogenin synthesis in liver. This led to an increase in liver wet weight and total DNA. After incubation with micrococcal nuclease (EC 3.1.31.1) less soluble chromatin was obtained from nuclei of the estradiol treated than the control fish, but active gene regions were solubilized by the nuclease. Thus, in the estradiol treated fish soluble mononucleosomes contained hybridizable vitellogenin gene sequences. As a result of estradiol treatment the content in total liver of putrescine rose 3-fold, that of spermidine 2-fold, while spermine was unchanged. In muscle no significant changes were observed. The regulatory functions of polyamines during gene expression were investigated by binding (14C)spermine to isolated liver nuclei depleted of endogenous polyamines. The number of binding sites was higher in nuclei of estradiol treated than control fish. (14C)spermine associated preferentially with micrococcal nuclease insensitive chromatin. Thus, the high content of putrescine and spermidine in liver supported the view of polyamine accumulation in proliferating tissues. The preferential binding to condensed chromatin indicated a stabilizing effect of polyamines on the organization of inactive chromatin structures.Abbreviations MNase
micrococcal nuclease
- PMSF
phenylmethylsulfonylfluoride 相似文献