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Alemeh Rafaee Anahita Mohseni Meybodi Parichehreh Yaghmaei Seyedeh Hanieh Hosseini Marjan Sabbaghian 《Molecular reproduction and development》2020,87(2):251-259
SEPT12 is a testis‐specific gene involved in the terminal differentiation of male germ cells. SEPT12 protein is required for sperm head‐tail formation and acts as a fundamental constituent of sperm tail annulus. In this study, we screened genetic variations in exons 5, 6, 7 of the SEPT12 and assessed the annulus status in teratozoospermic, globozoospermic, and patients with immotile short tail sperm. DNA sequencing was performed for 90 teratozoospermic and 30 normozoospermic individuals. Immunocytochemistry, transmission electron microscopy and western blotting were conducted to evaluate annulus status and the expression level of SEPT12 in patients with a distinct exonic variation (c.474G>A), respectively. Five polymorphisms identified within the desired regions of the SEPT12, among them c.474G>A had the potential to induce aberrant splicing results in the expression of a truncated protein. The annulus was detected in most of the spermatozoa from teratozoospermic and normozoospermic men with c.474G>A. In contrast, in the patient with short tail sperm defect carrying c.474G>A, 99% of spermatozoa were devoid of the annulus. Based on our findings there would be no association between exons 5, 6, 7 polymorphisms of the SEPT12 gene and the occurrence of mentioned disease but c.474G>A would be a predisposing factor in male infertility. 相似文献
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Zahra Alimanesh Zeynab Alimanesh Fatemeh Davari Saeedeh Shadmehri Mozhgan Ahmadi Seyed Ali Hosseini Sepideh Dolati Alemeh Hariri Far 《Molecular & cellular biomechanics : MCB》2020,17(4):155-163
Diabetes mellitus (DM) disease can affect process of apoptosis by
increasing oxidative stress, nevertheless exercise and crocin can improve apoptosis; therefore present study aimed to investigate the effect of continued training
with crocin on apoptosis markers in liver tissue of diabetic rats. In this experimental study 32 diabetic rats based on fasting glucose divided into four groups of
eight rats including: 1) sham, 2) training, 3) crocin, and 4) training with crocin
also for investigate the effect of DM induction on apoptosis markers, eight healthy
rats assigned in healthy control group. During eight weeks groups 2 and 4 ran
60 minutes on treadmill with intensity of 50–55% maximum speed for three sessions per week and groups 3 and 4 received 25 mg/kg/day crocin peritoneally.
Shapiro–Wilk, one-way ANOVA with Tukey’s post-hot tests were used for statistical analysis of data (P ≤ 0.05). DM induction significantly increased Bcl-2 as
well as decreased Bax and P52 (P ≤ 0.05) nevertheless training and training with
crocin significantly decreased Bcl-2 and increased Bax and P53 (P ≤ 0.05); crocin
significantly decreased Bcl-2 and increased P53 (P ≤ 0.05) and training with crocin had higher effect on increase of Bax and P53 compare to training (P ≤ 0.05)
also increase of Bax compare to crocin. Although training and crocin alone can
improve apoptotic markers in diabetic rats, nevertheless training simultaneously
with crocin have better effects than training alone. 相似文献
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