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Albulov A. I. Frolova M. A. Varlamov V. P. Kovaleva E. I. Eliseev A. K. 《Applied Biochemistry and Microbiology》2022,58(4):490-491
Applied Biochemistry and Microbiology - Currently biostimulants based on chitosan and its derivatives are increasingly used in veterinary medicine. Chitosan and its modifications have many... 相似文献
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A. I. Albulov M. A. Frolova A. V. Grin E. I. Kovaleva N. V. Melnik P. A. Krasochko 《Applied Biochemistry and Microbiology》2018,54(5):518-521
Probability-based surveys of the application of chitosan succinate as an adjuvant added to the vaccine against animal necrobacteriosis have been performed. The addition of 3.7% chitosan succinate (MM 330 kDa) to the standard-dose vaccine formulation could contribute to improvement of the vaccine immunogenicity, as measured by the hemagglutination test (HA): 1 : 512 as compared to 1 : 128 in the control range. The use of 0.5-% chitosan succinate solution as a protective medium for drying in the production of a dried inactivated vaccine against infectious bovine rhinotracheitis could ensure the preservation of the biopreparation’s high immunogenic activity. Chitosan succinate was shown to have quite good solubility in water, allowing the possible use of saline solution to reconstitute the vaccine into the suspension for injection and to abandon expensive solvents. 相似文献
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Vasiukova NI Chalenko GI Gerasimova NG Perekhod EA Ozeretskovskaia OL Il'ina AV Varlamov VP Albulov AI 《Prikladnaia biokhimiia i mikrobiologiia》2000,36(4):433-438
Water-soluble low-molecular-weight (3-10 kDa) chitosan obtained by enzymatic degradation of high-molecular-weight chitosan, as well as its deaminated derivatives, can be used as elicitors of late blight resistance in potato. 相似文献
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E. S. Saltykova A. A. Karimova A. R. Gataullin L. R. Gaifullina R. T. Matniyazov M. A. Frolova A. I. Albulov A. G. Nikolenko 《Applied Biochemistry and Microbiology》2016,52(5):553-557
High-molecular weight chitosan (200 kDa, 75% deacetylated) and N-succinoyl chitosan (300 kDa, 75% deacetylated) were shown to have a preadaptive effect and increase the lifespan of honeybees due to the induction of protective antioxidant and immune mechanisms. Chitosan with a molecular weight of 200 kDa had a fungistatic effect on a pathogenic fungus that causes ascospherosis, a disease of bee larvae and pupae. 相似文献
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N. I. Vasyukova G. I. Chalenko N. G. Gerasimova E. A. Perekhod O. L. Ozeretskovskaya A. V. Il’ina V. P. Varlamov A. I. Albulov 《Applied Biochemistry and Microbiology》2000,36(4):372-376
Water-soluble low-molecular-weight (3–10 kDa) chitosan obtained by enzymatic degradation of high-molecular-weight chitosan,
as well as its deaminated derivatives, can be used as elicitors of resistance to late blight in potato. 相似文献
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A. V. Ilyina D. S. Zagorskaya A. N. Levov A. I. Albulov N. P. Kovacheva V. P. Varlamov 《Applied Biochemistry and Microbiology》2009,45(4):374-379
This article describes the optimal conditions for the enzymatic hydrolysis of chitosan and its chemically-modified derivatives using the preparation extracted from the king crab hepatopancrease possessing pronounced hydrolythic activity. The following preparations were used: chitosan with a molecular weight of 700 kDa and an acetylation level of 0.15, carboxymethyl chitosan 200 kDa witih an extent of replacement of 0.23, and N-succinyl chitosan 390 kDa with an extent of replacement of 0.8. Low molecular-weight samples of chitosan and of its modified derivatives were obtained with the yields of 85, 55, and 80%, respectively. The conditions of the hydrolysis were as follows: an enzyme: substrate ratio of 1: 200, 37°C, and 20 h duration of hydrolysis. 相似文献
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Albulov A. I. Frolova M. A. Zelinskaya E. I. Eliseev A. K. 《Applied Biochemistry and Microbiology》2022,58(3):343-345
Applied Biochemistry and Microbiology - The effect of chitosan on the survival of bees treated with the chemical acaricide amitraz at a 50% lethal dose (LD50) of 10 μg per bee was studied. It... 相似文献
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S. V. Nemtsev O. Yu. Zueva M. R. Khismatullin A. I. Albulov V. P. Varlamov 《Applied Biochemistry and Microbiology》2004,40(1):39-43
A procedure of isolation of chitin, chitosan, and water-soluble low-molecular-weight chitosan from the corpses of bees has been developed. This procedure includes deproteinization of bee corpses, discoloration of the chitin–melanin complex, deacetylation, and enzymatic hydrolysis of chitosan. 相似文献
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