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Solange Faria Lua Figueiredo Norma Albarello Vera Regina Campos Viana 《In vitro cellular & developmental biology. Plant》2001,37(4):471-475
Summary A protocol for in vitro propagation of Rollinia mucosa, an important medicinal plant, was developed. The presence of 500 mg l−1 polyvinylpyrrolidone (PVP) during explant excision was important to avoid browning. Axillary buds, adventitious buds, and
shoot cluster proliferation were achieved from epicotyl and hypocotyl explants from nursery-grown seedlings. The highest direct
organogenesis percentage from hypocotyl explants was obtained upon culture of explants on Murashige and Skoog medium supplemented
with 2.2 μM benzyladenine (BA) plus 2.32 μM kinetin. Epicotyl explants display highest regeneration frequency on a medium containing 8.8 μM BA and 0.54 μM naphthaleneacetic acid. Gibberellic acid was necessary for shoot elongation. Root induction was observed when shoots were
pretreated with activated charcoal for 7 d in the dark before culture on Woody Plant Medium supplemented with 49.21 μM indolebutyric acid for 10 d. Root development was observed when 20 g l−1 sucrose was used. Rooted plantlets were acclimatized and grown in the greenhouse. 相似文献
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Faria Lua Figueiredo Solange Regina Campos Viana Vera Simões Cláudia Albarello Norma Carlos Trugo Luiz Auxiliadora Coelho Kaplan Maria Krul William Robert 《Plant Cell, Tissue and Organ Culture》1999,56(2):121-124
Rollinia mucosa produces furofuranic lignans (magnolin, epiyangambin, yangambin) that are antagonists of platelet-activating
factor (PAF). The biosynthetic capacity and the potential for the accumulation of furofuranic lignans, including epieudesmin,
of the plants cultured both in vivo and in vitro conditions were evaluated. The production and the pattern of lignans accumulated
were dependent on the origin of the plant material and the plant organ. The major accumulation of lignans was observed in
leaves. In the mature leaves of in vivo grown seedlings magnolin and yangambin predominated, in contrast to leaves from in
vitro propagated plants that presented epiyangambin as the major lignan.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
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Simões-Gurgel Claudia Cordeiro Lívia da Silva de Castro Tatiana Carvalho Callado Cátia Henriques Albarello Norma Mansur Elisabeth 《Plant Cell, Tissue and Organ Culture》2011,106(3):537-545
The effects of different levels of Murashige and Skoog (MS) basal medium, 2,4-dichlorophenoxyacetic acid (2,4-D), and sucrose
on anthocyanin production and biomass accumulation of cell suspension cultures of Cleome rosea were investigated. Cultures were established in liquid MS medium containing 30 g l−1 sucrose and supplemented with 0.90 μM 2,4-D. Proliferating cell suspension cultures achieved the highest growth capacity,
a fourfold increase in biomass accumulation, following subculture at the exponential growth phase, 14–18 days of culture.
Moreover, the presence of 2,4-D was essential for anthocyanin production and biomass accumulation. On the other hand, increasing
levels of sucrose above 30 g l−1 resulted in a drastic reduction in biomass accumulation. Anthocyanin production was highest in cell suspension cultures grown
on half-strength MS medium (1/2 MS), 30 g l−1 sucrose, and 0.45 μM 2,4-D. These cell suspension cultures were mainly composed of small aggregates of spherical cells with
similar morphology observed in anthocyanin-producing and non-producing cultures. Moreover, microscopic analysis of anthocyanin-producing
cultures showed the presence of mixtures of non-pigmented, low-pigmented, and high-pigmented cells. 相似文献
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Claudia Simões Tatiana Carvalho de Castro Lívia da Silva Cordeiro Norma Albarello Elisabeth Mansur Maria Teresa Villela Romanos 《World journal of microbiology & biotechnology》2010,26(1):93-99
Extracts from Cleome rosea were investigated for their activity against acyclovir-resistant strains of Herpes simplex type 1 (ACVr-HSV-1) and type 2 (ACVr-HSV-2). Methanolic and acidified (1% (v/v) HCl) methanolic extracts were prepared from
field-grown plants and in vitro propagated plants, as well as from calli and cell suspension cultures. The extracts presented
low cytotoxicity and caused virus titer reduction above 70%, with different mechanisms of action. Extracts from leaves of
field-grown plants inhibited viral infection mainly by affecting the virus particle itself (virucidal effect), while extracts
from calli acted mainly on cell receptors. On the other hand, all extracts evaluated affected the virus entry across the cell
membrane and the intracellular viral replication at similar percentages, causing reduction on titers in the range of 68–90%.
This study validated the potential use of in vitro materials as sources of antiherpetic agents from C. rosea. 相似文献
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da Silva Cordeiro Lívia Collin Myriam Callado Cátia Henriques Simões-Gurgel Claudia Albarello Norma Engelmann Florent 《Protoplasma》2020,257(4):1021-1033
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Figueiredo Solange Faria Lua Simões Cláudia Albarello Norma Campos Viana Vera Regina 《Plant Cell, Tissue and Organ Culture》2000,63(2):85-92
Different types and concentrations of plant growth regulators were tested in order to obtain the best callus and cell suspension
culture growth conditions of Rollinia mucosa (Jacq.) Baill. (Annonaceae). Picloram was shown to be the most efficient for induction and production of friable calluses,
independent of the concentration used. Cellular morphology and viability, fresh and dry weights, pH and medium sugar concentration
were determined for cell suspension cultures. Dissimilation curves were used for the characterization of the growth of cell
suspension cultures. Picloram provided the most rapid growth and produced the highest biomass, with little variation in morphology
(differentiated cells). It also provided the highest dissimilation, when compared with cell suspension cultures maintained
in media with 2,4-D or NAA + BA + GA3. Stable cell suspension cultures can be established in MS medium supplemented with 20.8 μM picloram.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
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Malecky M Albarello H Broudiscou LP 《Animal : an international journal of animal bioscience》2012,6(4):612-616
This in vitro study aimed at estimating the disappearance rates of 14 terpenes and terpenoids after 24-h incubation with mixed bacteria from caprine rumens. These compounds comprised nine monoterpene hydrocarbons (δ-3-carene, p-cymene, β-myrcene, (E)- and (Z)-β-ocimene, α-phellandrene, α-terpinene, γ-terpinene and α-terpinolene), four oxygenated monoterpenes ((E)- and (Z)-linalool oxide, 4-terpinenol, α + γ terpineol) and one sesquiterpene hydrocarbon (β-cedrene). They were individually exposed to goat rumen microflora for 24 h in 70 ml culture tubes at an input level of 0.5 ml/l. Terpenoids were the least degraded, 100% of (E)-linalool oxide, 95% of (Z)-linalool oxide, 91% of 4-terpinenol and 75% of terpineol remained intact after 24-h incubation. In contrast, α-terpinolene concentration in fermentation broth extracts was below quantification limit, thus indicating an extensive, if not complete, degradation by rumen bacteria. Only 2% of the initial amounts of α-phellandrene were recovered. The other monoterpenes and β-cedrene were partly degraded, with losses ranging from 67% for δ-3-carene to 90% for (E)-β-ocimene. The corresponding rates of disappearance were between 2.67 and 4.08 μmol/ml inoculum per day. 相似文献
10.
Righi E Giacomazzi CG Lindstrom V Albarello A Soro O Miglino M Perotti M Varnier OE Gobbi M Viscoli C Bassetti M 《Mycopathologia》2008,165(6):407-410
Cunninghamella bertholletiae infection occurs most frequently in neutropenic patients affected by haematological malignancies, is associated with an unfavourable outcome. We report a case of rhino-mastoidal fungal infection in a leukaemic patient. Bioptical tissue cultures yield the isolation of a mould with typical properties of Cunninghamella species. Liposomal amphotericin B (L-Amb) therapy combined with surgical intervention brought the lesion to recovery. Nevertheless, the patient died 14 days after bone marrow transplantation (BMT) from bacterial sepsis. Mastoiditis was documented at CT-scan. The conditioning regimen probably caused the reactivation of the Cunninghamella infection that led to the patient's fatal outcome; fungal hyphae were detected after autopsy of brain and lung tissue. 相似文献