全文获取类型
收费全文 | 1870篇 |
免费 | 223篇 |
出版年
2022年 | 11篇 |
2021年 | 28篇 |
2020年 | 19篇 |
2019年 | 21篇 |
2018年 | 25篇 |
2017年 | 23篇 |
2016年 | 35篇 |
2015年 | 74篇 |
2014年 | 73篇 |
2013年 | 78篇 |
2012年 | 92篇 |
2011年 | 114篇 |
2010年 | 80篇 |
2009年 | 53篇 |
2008年 | 111篇 |
2007年 | 91篇 |
2006年 | 88篇 |
2005年 | 78篇 |
2004年 | 95篇 |
2003年 | 77篇 |
2002年 | 92篇 |
2001年 | 43篇 |
2000年 | 37篇 |
1999年 | 45篇 |
1998年 | 30篇 |
1997年 | 22篇 |
1996年 | 21篇 |
1995年 | 24篇 |
1994年 | 19篇 |
1993年 | 23篇 |
1992年 | 19篇 |
1991年 | 20篇 |
1990年 | 27篇 |
1989年 | 21篇 |
1988年 | 17篇 |
1987年 | 19篇 |
1986年 | 15篇 |
1985年 | 20篇 |
1984年 | 23篇 |
1983年 | 14篇 |
1982年 | 22篇 |
1981年 | 12篇 |
1979年 | 18篇 |
1978年 | 17篇 |
1977年 | 22篇 |
1976年 | 18篇 |
1975年 | 16篇 |
1974年 | 18篇 |
1973年 | 18篇 |
1972年 | 11篇 |
排序方式: 共有2093条查询结果,搜索用时 0 毫秒
1.
2.
3.
4.
5.
Specific interaction of vinculin with alpha-actinin 总被引:33,自引:0,他引:33
D H Wachsstock J A Wilkins S Lin 《Biochemical and biophysical research communications》1987,146(2):554-560
Vinculin and alpha-actinin are cytoskeletal proteins present at focal contacts of the ventral surface of cultured fibroblasts. We labelled alpha-actinin with an acceptor fluorophore and vinculin with a donor. A mixture of vinculin and alpha-actinin showed a 28% quench, due to energy transfer, suggesting an interaction. Quench of vinculin was dependent on the concentration of alpha-actinin; Scatchard analysis gives a dissociation constant in the microM range. Quench was inhibited by excess unlabelled alpha-actinin, and by reaction of the acceptor protein with p-chloromercuribenzoate. We found that vinculin had a slightly greater elution volume in a gel filtration column equilibrated with alpha-actinin, indicating a higher effective Stokes radius due to the interaction of the two proteins. 相似文献
6.
7.
Cecilia PC Soh Alastair SR Donald James Feeney Walter TJ Morgan Winifred M Watkins 《Glycoconjugate journal》1989,6(3):319-332
The tetrasaccharides GalNAcß1-4[NeuAc2-3]Galß1-4Glc and GalNAcß1-4[NeuAc2-3]Galß1-4GlcNAc were synthesised by enzymic transfer of GalNAc from UDP-GalNAc to 3-sialyllactose (NeuAc2-3Galß1-4Glc) and 3-sialyl-N-acetyllactosamine (NeuAc2-3Galß1-4GlcNAc). The structures of the products were established by methylation and1H-500 MHz NMR spectroscopy. In Sda serological tests the product formed with 3-sialyl-N-acetyllactosamine was highly active whereas that formed with 3-sialyllactose had only weak activity. 相似文献
8.
Anna L. Ballard Alastair G. McEwan David J. Richardson J. Baz Jackson Stuart J. Ferguson 《Archives of microbiology》1990,154(3):301-303
Rhodobacter capsulatus strain BK5 possesses a membrane bound respiratory nitrate reductase rather than the periplasmic enzyme found in other strains. The enzyme in strain BK5 is shown to be both functionally and structurally related to the nitrate reductase of Paracoccus denitrificans and Escherichia coli.Abbreviation TMAO
trimethylamine-N-oxide 相似文献
9.
Dr. John L. Johnson Carol Phelps Lisa Barroso Mary D. Roberts David M. Lyerly Tracy D. Wilkins 《Current microbiology》1990,20(6):397-401
Results from our cloning studies on toxin A indicated that the gene for toxin B resided approximately 1 kb upstream of the toxin A gene. Clone pCD19, which contains the 5-end of the toxin A gene and a small open reading frame, was found to contain 1.2 kb of DNA which, when subcloned, expressed a nontoxic peptide that reacted with toxin B antibodies. The rest of the toxin B gene was located on the 6.8 kb cloned fragment of plasmid pCD19L. The two fragments overlapped 0.8 kb. Lysates containing protein expressed by the 6.8 fragment were cytotoxic and lethal, and were neutralized by toxin B antibody. The two fragments were ligated to give the complete toxin B gene. The protein expressed by the complete gene was cytotoxic and lethal, and showed complete immunological identity with toxin B. Further analysis of the expressed protein and the toxin B gene confirmed our earlier findings showing that toxin B has a molecular weight of 240,000 or greater. 相似文献
10.