Stereoselective HPLC analysis of tertatolol in rat plasma using macrocyclic antibiotic chiral stationary phase

Enantiomeric resolution of teratolol was achieved on a vancomycin macrocyclic antibiotic chiral stationary phase known as Chirobiotic V with UV detection set at 220 nm. The polar ionic mobile phase (PIM) consisted of methanol-glacial acetic acid-triethylamine (100:0.01:0.015, v/v/v) has been used at a flow rate of 0.8 ml min(-1) . The calibration curves in plasma were linear over the range of 5-500 ng ml(-1) for each enantiomer with detection limit of 2 ng ml(-1) . The proposed method was validated in compliance with the international conference on harmonization (ICH) guidelines. The developed method applied for the trace analyses of tertatolol enantiomers in plasma and for the pharmacokinetic study of tertatolol enantiomers in rat plasma. The assay proved to be suitable for therapeutic drug monitoring and chiral quality control for tertatolol formulations by HPLC.     

烟草黄矮双生病毒中双向启动子的活性及其调节控制

将烟草黄矮双生病毒（ＴｏｂＹＤＶ）中双向启动子的区域,以不同长度的片段和方向插入启动子分析载体ｐＧ１,与ＧＵＳ报道基因和ＮＯＳ终止子融合。同时,将各个ＴｏｂＹＤＶ读码框区域插入表达载体ｐＡＲＴ７中,置于ＣａＭＶ３５Ｓ启动子和ＯＣＳ终止子之间。用电穿孔法将各种启动子构建物个别地或者与读码框构建物成对地导入烟草和玉米原生质体,以考察ＴｏｂＹＤＶ启动子控制下ＧＵＳ基因瞬间表达的活性,以及ＴｏｂＹＤＶ的读