Poly a blocks in the nuclear ribonucleoprotein complexes,containing pre-mRNA

Poly A was found in nuclear particles containing pre-mRNA. It was shown that during the isolation of 30S particles from rat liver or Ehrlich ascites carcinoma nuclei, all poly A is detached from the particles containing pre-mRNA and is found in the form of RNP with a sedimentation coefficient of about 14S. When RNP polyparticles are isolated in the presence of RNase inhibitor poly A is distributed among the particles of higher molecular weights.Since the sedimentation properties and buoyant density of the poly A-containing particles are different from the 30S particles it was suggested that the poly A fragments are bound not with informofers, but with another kind of protein.     

Association of combinations of polymorphisms in fibroblast growth factor receptor 2 gene with breast cancer among various ethnic groups

A search for association of polymorphisms in loci rs2981582, rs2420946, rs17102287, rs1219648, rs2981578, and rs17542768 in fibroblast growth factor receptor 2 (FGFR2) gene was performed using the “case-control” method among the major ethnic groups of Kazakhstan. Restriction fragments length polymorphism analysis of DNA obtained from 495 Kazakh and 195 Russian women with breast cancer (BC) revealed no statistically significant differences compared with the control group (190 Kazakhs, 170 Russians). An assessment of combination associations of alleles and genotypes (patterns) with BC was performed using the APSampler algorithm. Association of risk type (OR > 1.4; P _Fischer < 0.05; P _permutation < 0.05) in the Kazakh group identified two patterns, including allele C at site rs2420946, while the T allele was present in four protective patterns (OR < 0.70). No significant association of the examined polymorphisms with BC risk in the Russian group was revealed.     

Polymorphic markers of some genes associated with multiple sclerosis in the population of Kazakhstan

Associations of DR2 specificity of the DRB1 gene and single-nucleotide polymorphisms of the tumor necrosis factor gene TNFα (−308 G/A), interleukin genes IL-1β (−511 C/T), IL-2 (−475 A/T and −631 G/A), IL-6 (−634 C/G), paraoxanase gene PON1 (M55L, Q192R), and the mitochondrial protein transport gene UCP2 (−866 G/A) with the development of multiple sclerosis (MS) were studied in two main ethnic groups of Kazakhstan (Kazakhs and Russians). An association of DR2 specificity of the DRB1 gene with MS was found in the combined group of Kazakhs, Russians, and offsprings from mixed marriages. No correlation between DR2 specificity and MS was found in the separately examined groups of Kazakhs and Russians. Statistically significant (p < 0.05) differences between the MS patients and healthy individuals were observed in the distribution of the genotypes at site −634 G/C of the IL-6 gene in the Kazakh group, in the allelic frequencies at site −308 A/G in the promoter region of the TNFα gene in the Russian group, and in the frequencies of alleles at the polymorphic Q192R locus of the PON1 gene in the Kazakh group.. No significant differences were revealed in the distribution of the genotypes and in the frequencies of alleles at the polymorphic sites of the genes IL-1β (−511 C/T), IL-2 (−475 A/T and −631 G/A), PON1 (M55L), and UCP2 (−866 G/A).     

Kaiso, a New Protein of the BTB/POZ Family, Specifically Binds to Methylated DNA Sequences

A protein specifically binding to a symmetrically methylated DNA fragment of the first intron of the mts1gene was studied. The protein was purified by gel filtration and affinity chromatography. Mass spectrometry showed that the protein is Kaiso, a new member of the BTB/POZ family. To study the association with methylated DNA sequences in vivo, the location of Kaiso in NIH 3T3 cells was analyzed. Immunofluorescent staining with polyclonal antibodies against Kaiso showed that the protein is predominantly associated with the nucleoli. The causes of its distribution awaits further investigation. The zinc-finger domains of Kaiso were for the first time demonstrated to specifically recognize symmetrically methylated DNA sequences in vitro.     

Polymorphism of the Promoter Region of the Angiotensinogen Gene and the Gene for Angiotensin I-Converting Enzyme in Arterial Hypertension and Cardiovascular Disease of the Kazakh Ethnic Group

Polymorphism of the promoter region of the angiotensinogen gene (ATG) and an angiotensin I-converting enzyme gene (ACE) insertion/deletion (I/D) polymorphism were studied in three different groups of Kazakhs (control group, patients with cardiovascular disease (CAD) and patients with arterial hypertension (AH)) using three methods. A comparative analysis of the distribution of genotype and allele frequencies was conducted.