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将菠菜叶片匀浆后.用差速离心和梯度率心分离叶绿体、过氧物酶体、微粒体等细胞器和100000×g上清法部分。用酶活测定法测定各部分甜菜碱醛脱氢酶(BADH)的活性;用免疫扩散法鉴定各组分的BADH。除叶绿体外,过氧物酶体、微粒体.以及100000×g上清液中也存在BADH。 相似文献
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Wu Changwei W. Tsai Pei-Jung Chen Sharon Chia-Ju Li Chia-Wei Hsu Ai-Ling Wu Hong-Yi Ko Yu-Ting Hung Pai-Chuan Chang Chun-Yen Lin Ching-Po Lane Timothy J. Chen Chia-Yuen 《Sleep and biological rhythms》2019,17(4):423-431
Sleep and Biological Rhythms - Neurovascular coupling (NVC), the transient regional hyperemia following the evoked neuronal responses, is the basis of blood oxygenation level-dependent techniques... 相似文献
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Xiao-Lin Shen Jin-Feng Yuan Xuan-He Qin Guang-Ping Song Huai-Bin Hu Hai-Qing Tu Zeng-Qing Song Pei-Yao Li Yu-Ling Xu Sen Li Xiao-Xiao Jian Jia-Ning Li Chun-Yu He Xi-Ping Yu Li-Yun Liang Min Wu Qiu-Ying Han Kai Wang Ai-Ling Li Tao Zhou Yu-Cheng Zhang Na Wang Hui-Yan Li 《The Journal of cell biology》2022,221(1)
Primary cilia transduce diverse signals in embryonic development and adult tissues. Defective ciliogenesis results in a series of human disorders collectively known as ciliopathies. The CP110–CEP97 complex removal from the mother centriole is an early critical step for ciliogenesis, but the underlying mechanism for this step remains largely obscure. Here, we reveal that the linear ubiquitin chain assembly complex (LUBAC) plays an essential role in ciliogenesis by targeting the CP110–CEP97 complex. LUBAC specifically generates linear ubiquitin chains on CP110, which is required for CP110 removal from the mother centriole in ciliogenesis. We further identify that a pre-mRNA splicing factor, PRPF8, at the distal end of the mother centriole acts as the receptor of the linear ubiquitin chains to facilitate CP110 removal at the initial stage of ciliogenesis. Thus, our study reveals a direct mechanism of regulating CP110 removal in ciliogenesis and implicates the E3 ligase LUBAC as a potential therapy target of cilia-associated diseases, including ciliopathies and cancers. 相似文献
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Chern CG Fan MJ Yu SM Hour AL Lu PC Lin YC Wei FJ Huang SC Chen S Lai MH Tseng CS Yen HM Jwo WS Wu CC Yang TL Li LS Kuo YC Li SM Li CP Wey CK Trisiriroj A Lee HF Hsing YI 《Plant molecular biology》2007,65(4):427-438
With the completion of the rice genome sequencing project, the next major challenge is the large-scale determination of gene
function. As an important crop and a model organism, rice provides major insights into gene functions important for crop growth
or production. Phenomics with detailed information about tagged populations provides a good tool for functional genomics analysis.
By a T-DNA insertional mutagenesis approach, we have generated a rice mutant population containing 55,000 promoter trap and
gene activation or knockout lines. Approximately 20,000 of these lines have known integration sites. The T0 and T1 plants
were grown in net “houses” for two cropping seasons each year since 2003, with the mutant phenotypes recorded. Detailed data
describing growth and development of these plants, in 11 categories and 65 subcategories, over the entire four-month growing
season are available in a searchable database, along with the genetic segregation information and flanking sequence data.
With the detailed data from more than 20,000 T1 lines and 12 plants per line, we estimated the mutation rates of the T1 population,
as well the frequency of the dominant T0 mutants. The correlations among different mutation phenotypes are also calculated.
Together, the information about mutant lines, their integration sites, and the phenotypes make this collection, the Taiwan
Rice Insertion Mutants (TRIM), a good resource for rice phenomics study. Ten T2 seeds per line can be distributed to researchers
upon request.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users.
Chyr-Guan Chern, Ming-Jen Fan, and Su-May Yu have contributed equally to this work. 相似文献
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Li WH Zhao J Li HY Liu H Li AL Wang HX Wang J He K Liang B Yu M Shen BF Zhang XM 《Proteomics》2006,6(17):4781-4789
The identification of panels of tumor antigens that elicit an antibody response may have utility in cancer screening, diagnosis and in establishing prognosis. However, autoantibodies normally exist in sera of healthy individuals and are enormously diversified. To explore the reservoir of autoantibody in healthy population, we performed a proteomics investigation of autoantibody profiles in the sera of 36 healthy Chinese individuals from Beijing, which may provide valuable reference information to the identification of disease-specific autoantibodies. The results showed that autoantibody profiles varied individually, but some autoantibodies were identified at a high frequency in the healthy population. The autoantibodies against alpha-enolase and those against heterogeneous nuclear ribonucleoprotein L were positive in more than 50% of the sera samples. The autoantibodies identified in more than 20% of samples included those against annexin II, F-actin capping protein beta subunit and calreticulin. Some of these autoantibodies have been previously reported to be involved in autoimmune conditions and cancers. Autoantibodies in the healthy population are important as a foundation from which disease-specific autoantibodies can be defined. Thus our report on autoantibodies in healthy individuals may be useful as a reference for defining new autoantibody biomarkers. 相似文献
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中国蓼属春蓼组植物果实形态及果皮微形态的研究 总被引:5,自引:1,他引:4
应用解剖镜和扫描电镜对中国产蓼属春蓼组(Polygonum sect. Persicaria)19种3变种植物的果实形态和果皮微形态特征进行了观察和研究。结果表明,蓼属春蓼组植物的果实形态为卵形或椭圆状卵形,具三棱、双凹或双凸,顶端渐狭,有喙或无喙;果皮微形态可分为7种类型: 洼点、浅洼点,脑纹状纹饰,拟脑纹状纹饰,网状纹饰,不规则褶皱,不规则小疣状颗粒以及密浅网状纹饰。观察结果支持将绵毛酸模叶蓼(P. lapathifolium L. var. salicifolium Sibth.)合并到酸模叶蓼(P. lapathifolium L.),做为酸模叶蓼的异名处理;支持将长鬃蓼(P. longisetum De Br.)作为丛枝蓼(P. posumbu Buch. Ham. ex D.Don)的变种处理的意见;认为密毛酸模叶蓼(P. lapathifolium L. var. lanatum (Roxb.) Stew.)应恢复种级,圆基长鬃蓼(P. longisetum De Br. var. rotunatum A.J.Li)应升为种级;支持平武蓼(Polygonum pingwuense F. Z. Li et Y. T. Hou et S. J. Fan, sp. nov.)新种的成立。 相似文献
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To facilitate genetic research, we constructed two linkage maps by employing two F? populations derived from rice inter-subspecific crosses, japonica Tainung 67 (TNG67)/indica Taichung Sen 10 (TCS10) and japonica TNG67/indica Taichung Sen 17 (TCS17). We established linkage map lengths of 1481.6 cM and 1267.4 cM with average intervals of 13.8 cM and 14.4 cM by using 107 and 88 PCR markers for coverage of 88% of the rice genome in TNG67/TCS10 and TNG67/TCS17, respectively. The discrepancy in genetic maps in the two populations could be due to different cross combinations, crossing-over events, progeny numbers and/or markers. The most plausible explanation was segregation distortion; 18 markers (16.8%) distributed at nine regions of seven chromosomes and 10 markers (11.4%) at four regions of four chromosomes displayed severe segregation distortion (p < 0.01)in TNG67/TCS10 and TNG67/TCS17, respectively. All segregation-distorted markers in these two populations corresponded to reported reproductive barriers, either gametophytic or zygotic genes but not to hybrid breakdown genes. The observed recombination frequency, which was higher or lower than the intrinsic frequency, revealed the association of segregation distortion skewed to the same or different genotypes at the consecutive markers. The segregation distortion, possibly caused by reproductive barriers, affects the evaluation recombination frequencies and consequently the linkage analysis of QTLs and positional cloning. 相似文献