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Asaph Ahroni Amir Zuker Yael Rozen Hadas Shejtman Alexander Vainstein 《Plant Cell, Tissue and Organ Culture》1997,49(2):101-106
An efficient adventitious shoot regeneration procedure was developed for Gypsophila paniculata L. Using cultivar Arbel, shoot
regeneration from the three upper internodes of the stem was monitored on MS media supplemented with different cytokinins
(thidiazuron, benzyladenine, kinetin or zeatin) and an auxin (naphthaleneacetic acid). Thidiazuron was found to be the most
efficient cytokinin, with up to 100% of the explants forming shoots, at an average of up to 19 shoots per explant being regenerated.
The highest percentage of shoot formation was observed in the stem explants originating from the first internode, with all
cytokinins tested. The adventitious origin of shoots regenerated from stem explants was confirmed by scanning electron microscopy.
The regeneration procedure was found to be applicable to five other gypsophila cultivars (Perfecta, Golan, Gilboa, Flamingo
and Tavor). Regenerating plants were successfully transferred to soil, and did not differ in flower color, size or shape from
standard vegetatively propagated plants derived from cuttings.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
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Zuker Amir Ahroni Asaph Tzfira Tzvi Ben-Meir Hagit Vainstein Alexander 《Molecular breeding : new strategies in plant improvement》1999,5(4):367-375
Highly efficient Agrobacterium-mediated transformation of carnation (Dianthus caryophyllus L.) was obtained by first wounding stem explants via microprojectile bombardment. When this was followed by cocultivation with disarmed Agrobacterium in the dark, the transformation frequency-based on transient GUS expression-increased to over 10-fold that of explants wounded by other means and cocultivated under constant light. Two cycles of regeneration/selection on kanamycin were employed to generate stably transformed carnation plants and eliminate chimeras: first, plantlets were regenerated from inoculated stem explants and then leaves from these plantlets were used to generate transgenes in a second selection cycle of adventitious shoot regeneration. Agrobacterium strain AGLO, carrying the binary vector pCGN7001 containing uidA and nptII genes, was used in the stable transformation experiments. The combination of wounding via bombardment, cocultivation in the dark and two cycles of kanamycin selection yielded an overall transformation efficiency of 1–2 transgenes per 10 stem explants for the three carnation varieties analyzed. Histochemical and molecular analyses of marker genes in T0 and T1 generations confirmed the transgenic nature of the selected plants. 相似文献
3.
Adventitious shoots were successfully regenerated from leaf explants of Gypsophila paniculata L. The efficiency of shoot regeneration for cv. Arbel was tested on 18 media based on Murashige and Skoog basal medium containing
different concentrations of thidiazuron or 6-benzylaminopurine in combination with naphthaleneacetic acid. Both explant age
and that of the cuttings used as leaf donors affected the regeneration efficiency. The highest efficiency of adventitious
shoot regeneration was obtained with the oldest leaves originating from the youngest cutting analyzed; on thidiazuron-containing
medium, shoots regenerated on average from 67% of the leaves, with an average of seven shoots per explant. This regeneration
procedure was suitable for all six commercial cultivars studied. Regenerated shoots elongated, rooted and successfully acclimatized
to the greenhouse where they were grown to flowering.
Received: 25 July 1998 / Revision received: 11 November 1996 / Accepted: 30 November 1996 相似文献
4.
Reuben Ahroni 《American anthropologist》2000,102(3):656-657
Holy Writ as Oral Lit: The Bible as Folklore. Alan Dundes. Lanham, MD: Rowman and Littlefield Publishers, 1999. 131 pp. 相似文献
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