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Adenise L. Woiciechowski Carlos R. Soccol Luis P. Ramos Ashok Pandey 《Process Biochemistry》1999,34(9):75
The fermentation of hemicellulosic hydrolysate from Pinus taeda chips, using the fungal culture Rhizopus oryzae, was carried out to produce l-(+)-lactic acid and to optimize and enhance the biological conversion of reducing sugar into l-(+)-lactic acid using the experimental design to evaluate the culture conditions. The first factorial design based on surface response with five factors (agitation level, substrate concentration, CaCO3 concentration, C/N and C/P ratios) at low levels and one medium point was performed to optimize culture conditions. The second study tested two factors (substrate concentration and C/N ratio) at three levels. The statistical analysis of the data obtained from the factorial study showed that a C/N ratio of 35 and substrate concentration of 90 g/litre were the best conditions to produce l-(+)-lactic acid with R. oryzae on P. taeda hydrolysate, but in this case the statistical projection was not correct and the real optimized conditions were C/N ratio of 55 and substrate concentration of 75 g/litre of reducing sugar. 相似文献
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Michele Rigon Spier Adenise Lorenci Woiciechowski Luiz Alberto Junior Letti Gessiel Newton Scheidt Wilerson Sturm Jose Angel Rodriguez-León Julio César de Carvalho Dario Eduardo Amaral Dergint Carlos Ricardo Soccol 《Bioprocess and biosystems engineering》2010,33(9):1033-1041
Fermentation parameters for phytase production in column-type bioreactor were monitored using a new data acquisition system. There are a number of studies reporting phytase production in flasks, but a lack of data about microorganism respiration behaviour during phytase production using column bioreactor. The objectives of this work were the monitoration of fermentation parameters during phytase production and its relation with fungal growth and forced air. Phytase production by A. niger FS3 increased with forced air. The O2 consumption and CO2 production during solid-state fermentation were monitored by sensors (in the bottom and top of the columns) linked to controllers, recorded by acquisition software and processed by Fersol2® software tool. Phytase synthesis was associated with fungal growth. Therefore, phytase could be used to estimate FS3 biomass formed in citric pulp degradation. 相似文献
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Dlugokenski RE Sella SR Guizelini BP Vandenberghe LP Woiciechowski AL Soccol CR Minozzo JC 《Applied microbiology and biotechnology》2011,90(2):713-719
A novel low-cost medium was developed from by-products and wastes from the ethanol agro-industry to replace commercial media
in the production of a steam sterilization biological indicator (BI). Various recovery media were developed using soybean
or sugarcane molasses and vinasse to prepare a self-contained BI. Media performance was evaluated by viability and heat resistance
(D
121 °C value) according to regulatory standards. A medium produced with a soybean vinasse ratio of 1:70 (1.4%) (w/v) produced the results, with D
121 °C = 2.9 ± 0.5 min and Usk = 12.7 ± 2.1 min. The addition of 0.8% (w/v) yeast extract improved the germination of heat-damaged spores. The pH variation from 6.0 to 7.3 resulted in a gradual increase
in the D
121 °C value. The absence of calcium chloride resulted in a decrease in germination, while no significant differences were observed
with starch addition. Soybean vinasses may thus be used as the main component of a culture medium to substitute for commercial
media in the production of self-contained biological indicators. The use of ethanol production waste in this biotechnological
process realized a reliable performance, minimized the environmental impact, and decreased BI production costs while producing
a high quality product. 相似文献
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M. R. Spier R. C. Fendrich P. C. Almeida M. Noseda R. Greiner U. Konietzny A. L. Woiciechowski V. T. Soccol C. R. Soccol 《World journal of microbiology & biotechnology》2011,27(2):267-274
Citric pulp is an agro-industrial residue from the citrus processing industry with low inorganic phosphorus content applied
in animal feed. A new bioprocess was developed to produce and purify a new phytase generated on citric pulp fermentation by
Aspergillus niger FS3. The phytase was purified by cationic-exchange, anionic-exchange chromatography and chromatofocusing steps. From SDS–PAGE
analysis, the molecular weight of the purified phytase was calculated to be 108 kDa. The phytase had an optimum pH of 5.0–5.5
and an optimum temperature of 60°C. The phytase displayed high affinity for phytate, and the K
m was 0.52 mM. The purified phytase was sufficiently able to withstand pelleting temperatures, retaining sufficiently high
phytate-degrading activity. 相似文献
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