排序方式: 共有4条查询结果,搜索用时 15 毫秒
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Santos Beatriz da Conceição Daniele Priscila Corrêa Diego Oliveira Passos Matheus Felipe Campos Monique Paiva Adamoski Douglas Galli-Terasawa Lygia Vitória Mariano André Bellin Vargas José Viriato Coelho Kava Vanessa Merlo 《Journal of applied phycology》2022,34(2):729-743
Journal of Applied Phycology - The present study evaluated the effect of blue, red, and white light on the expression of four carotenogenic genes (psy, lyc, bkt, and crtr-r); one gene related to... 相似文献
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Amanda Petrina Scotá Ferreira Alexandre Cassago Kaliandra de Almeida Gon?alves Marília Meira Dias Douglas Adamoski Carolline Fernanda Rodrigues Ascen??o Rodrigo Vargas Honorato Juliana Ferreira de Oliveira Igor Monteze Ferreira Camila Fornezari Jefferson Bettini Paulo Sérgio Lopes Oliveira Adriana Franco Paes Leme Rodrigo Villares Portugal Andre Luis Berteli Ambrosio Sandra Martha Gomes Dias 《The Journal of biological chemistry》2013,288(39):28009-28020
The phosphate-dependent transition between enzymatically inert dimers into catalytically capable tetramers has long been the accepted mechanism for the glutaminase activation. Here, we demonstrate that activated glutaminase C (GAC) self-assembles into a helical, fiber-like double-stranded oligomer and propose a molecular model consisting of seven tetramer copies per turn per strand interacting via the N-terminal domains. The loop 321LRFNKL326 is projected as the major regulating element for self-assembly and enzyme activation. Furthermore, the previously identified in vivo lysine acetylation (Lys311 in humans, Lys316 in mouse) is here proposed as an important down-regulator of superoligomer assembly and protein activation. Bis-2-(5-phenylacetamido-1,3,4-thiadiazol-2-yl)ethyl sulfide, a known glutaminase inhibitor, completely disrupted the higher order oligomer, explaining its allosteric mechanism of inhibition via tetramer stabilization. A direct correlation between the tendency to self-assemble and the activity levels of the three mammalian glutaminase isozymes was established, with GAC being the most active enzyme while forming the longest structures. Lastly, the ectopic expression of a fiber-prone superactive GAC mutant in MDA-MB 231 cancer cells provided considerable proliferative advantages to transformed cells. These findings yield unique implications for the development of GAC-oriented therapeutics targeting tumor metabolism. 相似文献
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Angela Cristina Ikeda Luciana Lange Bassani Douglas Adamoski Danyelle Stringari Vanessa Kava Cordeiro Chirlei Glienke Maria Berenice Reynaud Steffens Mariangela Hungria Lygia Vitoria Galli-Terasawa 《Microbial ecology》2013,65(1):154-160
Maize is one of the most important crops worldwide, and in Brazil, the state of Paraná stands as its largest producer. The crop demands high inputs of N fertilizers, therefore all strategies aiming to optimize the grain production with lower inputs are very relevant. Endophytic bacteria have a high potential to increment maize grain yield by means of input via biological nitrogen fixation and/or plant growth promotion, in this last case increasing the absorption of water and nutrients by the plants. In this study, we established a collection of 217 endophytic bacteria, isolated from roots of four lineages and three hybrid genotypes of maize, and isolated in four different N-free culture media. Biochemical―comprising growth in different carbon sources, intrinsic tolerance to antibiotics, and biochemical tests for catalase, nitrate reductase, urease, and growth in N-free media in vitro―and genetic characterization by BOX-PCR revealed great variability among the isolates. Both commercial hybrids and homozygous lineages were broadly colonized by endophytes, and sequencing of the 16S rRNA gene revealed the presence of bacteria belonging to the genera Pantoea, Bacillus, Burkholderia, and Klebsiella. Qualitative differences in endophytic colonization were detected between lineages and hybrid genotypes. 相似文献
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de Lima Franciéle Boldt Angelica Beate Winter Kava Vanessa Merlo Galli-Terasawa Lygia Vitória Adamoski Douglas 《Plant Molecular Biology Reporter》2022,40(3):471-481
Plant Molecular Biology Reporter - Nitrogen-fixing bacteria establish symbiosis with legumes for the biological nitrogen fixation process in specific root structures called nodules. Among these... 相似文献
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