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1.
Identification of resistance gene analogs linked to a powdery mildew resistance locus in grapevine 总被引:12,自引:6,他引:12
Donald TM Pellerone F Adam-Blondon AF Bouquet A Thomas MR Dry IB 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2002,104(4):610-618
Oligonucleotide primers, designed to conserved regions of nucleotide binding site (NBS) motifs within previously cloned pathogen
resistance genes, were used to amplify resistance gene analogs (RGAs) from grapevine. Twenty eight unique grapevine RGA sequences
were identified and subdivided into 22 groups on the basis of nucleic acid sequence-identity of approximately 70% or greater.
Representatives from each group were used in a bulked segregant analysis strategy to screen for restriction fragment length
polymorphisms linked to the powdery mildew resistance locus, Run1, introgressed into Vitis vinifera L. from the wild grape species Muscadinia rotundifolia. Three RGA markers were found to be tightly linked to the Run1 locus. Of these markers, two (GLP1–12 and MHD145) cosegregated with the resistance phenotype in 167 progeny tested, whereas
the third marker (MHD98) was mapped to a position 2.4 cM from the Run1 locus. The results demonstrate the usefulness of RGA sequences, when used in combination with bulked segregant analysis,
to rapidly generate markers tightly linked to resistance loci in crop species.
Received: 2 May 2001 / Accepted: 3 August 2001 相似文献
2.
Anchoring of a large set of markers onto a BAC library for the development of a draft physical map of the grapevine genome 总被引:3,自引:3,他引:0
Lamoureux D Bernole A Le Clainche I Tual S Thareau V Paillard S Legeai F Dossat C Wincker P Oswald M Merdinoglu D Vignault C Delrot S Caboche M Chalhoub B Adam-Blondon AF 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2006,113(2):344-356
Five hundred and six EST-derived markers, 313 SSR markers and 26 BAC end-derived or SCAR markers were anchored by PCR on a subset of a Cabernet Sauvignon BAC library representing six genome equivalents pooled in three dimensions. In parallel, the 12,351 EST clusters of the grapevine UniGene set (build #11) from NCBI were used to design 12,125 primers pairs and perform electronic PCR on 67,543 nonredundant BAC-end sequences. This in silico experiment yielded 1,140 positive results concerning 638 different markers, among which 602 had not been already anchored by PCR. The data obtained will provide an easier access to the regulatory sequences surrounding important genes (represented by ESTs). In total, 1,731 islands of BAC clones (set of overlapping BAC clones containing at least one common marker) were obtained and 226 of them contained at least one genetically mapped anchor. These assigned islands are very useful because they will link the genetic map and the future fingerprint-based physical map and because they allowed us to indirectly place 93 ESTs on the genetic map. The islands containing two or more mapped SSR markers were also used to assess the quality of the integrated genetic map of the grapevine genome.Electronic Supplementary Material Supplementary material is available to authorised users in the online version of this article at .Didier Lamoureux and Anne Bernole contributed equally to this work. 相似文献
3.
Loïc Le Cunff Alexandre Fournier-Level Valérie Laucou Silvia Vezzulli Thierry Lacombe Anne-Françoise Adam-Blondon Jean-Michel Boursiquot Patrice This 《BMC plant biology》2008,8(1):31
Background
The first high quality draft of the grape genome sequence has just been published. This is a critical step in accessing all the genes of this species and increases the chances of exploiting the natural genetic diversity through association genetics. However, our basic knowledge of the extent of allelic variation within the species is still not sufficient. Towards this goal, we constructed nested genetic core collections (G-cores) to capture the simple sequence repeat (SSR) diversity of the grape cultivated compartment (Vitis vinifera L. subsp. sativa) from the world's largest germplasm collection (Domaine de Vassal, INRA Hérault, France), containing 2262 unique genotypes. 相似文献4.
5.
A reference integrated map for cultivated grapevine (Vitis vinifera L.) from three crosses, based on 283 SSR and 501 SNP-based markers 总被引:1,自引:0,他引:1
Vezzulli S Troggio M Coppola G Jermakow A Cartwright D Zharkikh A Stefanini M Grando MS Viola R Adam-Blondon AF Thomas M This P Velasco R 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2008,117(4):499-511
We have developed an integrated map from five elite cultivars of Vitis vinifera L.; Syrah, Pinot Noir, Grenache, Cabernet Sauvignon and Riesling which are parents of three segregating populations. A new source of markers, SNPs, identified in ESTs and unique BAC-end sequences was added to the available IGGP reference set of SSRs. The complete integrated map comprises 1,134 markers (350 AFLP((R)), 332 BESs, 169 ESTs, 283 SSRs) spanning 1,443 cM over 19 linkage groups and shows a mean distance between neighbouring loci of 1.27 cM. Marker order was mainly conserved between the integrated map and the highly dense Syrah x Pinot Noir consensus map except for few inversions. Moreover, the marker order has been validated through the assembled genome sequence of Pinot Noir. We have also assessed the transferability of SNP-based markers among five V. vinifera varieties, enabling marker validation across different genotypes. This integrated map can serve as a fundamental tool for molecular breeding in V. vinifera and related species and provide a basis for studies of genome organization and evolution in grapevines. 相似文献
6.
Genetic and physical mapping of the grapevine powdery mildew resistance gene, Run1, using a bacterial artificial chromosome library 总被引:4,自引:0,他引:4
Barker CL Donald T Pauquet J Ratnaparkhe MB Bouquet A Adam-Blondon AF Thomas MR Dry I 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2005,111(2):370-377
Resistance to grapevine powdery mildew is controlled by Run1, a single dominant gene present in the wild grapevine species, Muscadinia rotundifolia, but absent from the cultivated species, Vitis vinifera. Run1 has been introgressed into V. vinifera using a pseudo-backcross strategy, and genetic markers have previously been identified that are linked to the resistance locus. Here we describe the construction of comprehensive genetic and physical maps spanning the resistance locus that will enable future positional cloning of the resistance gene. Physical mapping was performed using a bacterial artificial chromosome (BAC) library constructed using genomic DNA extracted from a resistant V. vinifera individual carrying Run1 within an introgression. BAC contig assembly has enabled 20 new genetic markers to be identified that are closely linked to Run1, and the position of the resistance locus has been refined, locating the gene between the simple sequence repeat (SSR) marker, VMC4f3.1, and the BAC end sequence-derived marker, CB292.294. This region contains two multigene families of resistance gene analogues (RGA). A comparison of physical and genetic mapping data indicates that recombination is severely repressed in the vicinity of Run1, possibly due to divergent sequence contained within the introgressed fragment from M. rotundifolia that carries the Run1 gene. 相似文献
7.
8.
Paux E Legeai F Guilhot N Adam-Blondon AF Alaux M Salse J Sourdille P Leroy P Feuillet C 《Functional & integrative genomics》2008,8(1):29-32
Anchored physical maps represent essential frameworks for map-based cloning, comparative genomics studies, and genome sequencing
projects. High throughput anchoring can be achieved by polymerase chain reaction (PCR) screening of bacterial artificial chromosome
(BAC) library pools with molecular markers. However, for large genomes such as wheat, the development of high dimension pools
and the number of reactions that need to be performed can be extremely large making the screening laborious and costly. To
improve the cost efficiency of anchoring in such large genomes, we have developed a new software named Elephant (electronic physical map anchoring tool) that combines BAC contig information generated by FingerPrinted Contig with results of BAC library pools screening to
identify BAC addresses with a minimal amount of PCR reactions. Elephant was evaluated during the construction of a physical map of chromosome 3B of hexaploid wheat. Results show that a one dimensional
pool screening can be sufficient to anchor a BAC contig while reducing the number of PCR by 384-fold thereby demonstrating
that Elephant is an efficient and cost-effective tool to support physical mapping in large genomes.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users.
E. Paux and F. Legeai contributed equally to this work. 相似文献
9.
J. Pauquet A. Bouquet P. This A.-F. Adam-Blondon 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,103(8):1201-1210
The monogenic dominant genetic determinism of total resistance to powdery mildew, introduced from Muscadinia
rotundifolia into Vitis
vinifera, was further assessed in BC4 and BC5 full-sib families. A BC5 population of 157 individuals was used to select AFLP markers linked to the resistance gene, Run1. Thirteen AFLP markers were selected and a local map was constructed around the Run1 gene. Ten markers among the 13 were found to co-segregate with the resistance gene. The usefulness of these 13 AFLP markers
for the selection of Run1-carrying genotypes was further assessed through their analysis in a set of 22 Run1-carrying resistant genotypes and 16 susceptible genotypes. Three markers out of the 13 analysed were found to be absent
in all susceptible genotypes and present in all resistant individuals, and may thus represent good tools for the marker-assisted
selection of grapevine varieties resistant to powdery mildew. A recombination event among the markers that were originally
found to co-segregate was observed in one of the resistant individuals, showing that recombination is possible in this region
and may therefore be observed in larger populations.
Received: 18 October 2000 / Accepted: 4 April 2001 相似文献
10.
V. Geffroy F. Creusot J. Falquet M. Sévignac A.-F. Adam-Blondon H. Bannerot P. Gepts M. Dron 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,96(3-4):494-502
Molecular markers offer new opportunities for breeding for disease resistance. Resistance gene pyramiding in a single cultivar,
as a strategy for durable resistance, can be facilitated by marker-assisted selection (MAS). A RAPD marker, ROH20450, linked to the Mesoamerican Co-2 anthracnose resistance gene, was previously transformed into a SCAR marker, SCH20. In the present paper we have further characterized
the relevance of the SCH20 SCAR marker in different genetic backgrounds. Since this SCAR marker was found to be useful mainly
in the Andean gene pool, we identified a new PCR-based marker (SCAreoli) for indirect scoring of the presence of the Co-2 gene. The SCAreoli SCAR marker is polymorphic in the Mesoamerican as well as in the Andean gene pool and should be useful
in MAS. We also report that PvH20, the cloned sequence corresponding to the 450-bp RAPD marker ROH20450, contains six imperfect leucine-rich repeats, and reveals a family of related sequences in the vicinity of the Co-2 locus. These results are discussed in the context of the recent cloning of some plant resistance genes.
Received: 26 June 1997 / Accepted: 13 October 1997 相似文献