全文获取类型
收费全文 | 1718篇 |
免费 | 250篇 |
国内免费 | 5篇 |
出版年
2023年 | 12篇 |
2022年 | 33篇 |
2021年 | 92篇 |
2020年 | 37篇 |
2019年 | 35篇 |
2018年 | 46篇 |
2017年 | 48篇 |
2016年 | 58篇 |
2015年 | 111篇 |
2014年 | 78篇 |
2013年 | 105篇 |
2012年 | 139篇 |
2011年 | 137篇 |
2010年 | 81篇 |
2009年 | 60篇 |
2008年 | 94篇 |
2007年 | 95篇 |
2006年 | 94篇 |
2005年 | 75篇 |
2004年 | 66篇 |
2003年 | 72篇 |
2002年 | 57篇 |
2001年 | 27篇 |
2000年 | 29篇 |
1999年 | 17篇 |
1998年 | 13篇 |
1997年 | 20篇 |
1996年 | 15篇 |
1995年 | 12篇 |
1994年 | 14篇 |
1993年 | 17篇 |
1992年 | 25篇 |
1991年 | 13篇 |
1990年 | 18篇 |
1989年 | 21篇 |
1988年 | 18篇 |
1987年 | 11篇 |
1986年 | 9篇 |
1985年 | 13篇 |
1984年 | 7篇 |
1983年 | 5篇 |
1981年 | 3篇 |
1979年 | 8篇 |
1978年 | 3篇 |
1977年 | 6篇 |
1975年 | 4篇 |
1973年 | 4篇 |
1972年 | 2篇 |
1971年 | 2篇 |
1970年 | 2篇 |
排序方式: 共有1973条查询结果,搜索用时 15 毫秒
1.
2.
3.
4.
Ethan R. Block Michael A. Tolino Jennifer S. Lozano Kira L. Lathrop Rebecca S. Sullenberger Abigail R. Mazie Jes K. Klarlund 《Molecular biology of the cell》2010,21(13):2172-2181
The ability of epithelia to migrate and cover wounds is essential to maintaining their functions as physical barriers. Wounding induces many cues that may affect the transition to motility, including the immediate mechanical perturbation, release of material from broken cells, new interactions with adjacent extracellular matrix, and breakdown of physical separation of ligands from their receptors. Depending on the exact nature of wounds, some cues may be present only transiently or insignificantly. In many epithelia, activation of the epidermal growth factor receptor (EGFR) is a central event in induction of motility, and we find that its continuous activation is required for progression of healing of wounds in sheets of corneal epithelial cells. Here, we examine the hypothesis that edges, which are universally and continuously present in wounds, are a cue. Using a novel culture model we find that their presence is sufficient to cause activation of the EGFR and increased motility of cells in the absence of other cues. Edges that are bordered by agarose do not induce activation of the EGFR, indicating that activation is not due to loss of any specific type of cell–cell interaction but rather due to loss of physical constraints. 相似文献
5.
6.
7.
8.
Pseudomonas sp. strains, isolated from soil, utilized toluene as their sole carbon source through ameta cleavage pathway. Strains metabolizing toluene through anortho cleavage pathway were selected from the wild typemeta strain. Theortho pathway strains were subjected to chemostat selection to obtain a fast-growing strain with doubling time reduced from 14 to 1.2 h. Benzoale and antibiotics enrichment selection procedures were utilized to select a blocked mutant. The blocked mutant grew on acetate as its sole carbon source and oxidatively converted toluene tocis, cis-muconic acid. Double-blocked and muconate-permeable mutants were also selected to reduce reversion frequency and to enhance muconic acid production. In shake-flask experiments, muconic acid at 3.5 g/l was obtained after 2 days of fermentation. In a 14 l fermenter, muconic acid was produced at 45 g/l in 4 days of controlled fed-batch fermentation. The oxidative bioconversion process was also demonstrated in a 1500 l fermenter.
Résumé Des souches dePseudomonas sp., isolées du sol, ont utilisé le toluène comme seule source de carbone par la vole de la rupture de cycle enmeta. On a sélectionné des souches métabolisant le toluène par la voie de la rupture de cycle enortho, à partir de la souche sauvage de typemeta. Les souches de la voieortho ont été soumises à la sélection en chémostat pour obtenir des souches à croissance rapide dont le temps de doublement est rédult de 14 à 1.2 h. Les procédures de sélection par enrichissement sur benzoale et antibiotiques ont été utilisées pour sélectionner un mutant bloqué. Le mutant bloqué croît sur acétate comme seule source de carbone et convertit le toluène par voie oxydative en acidecis,cis-muconique. On a également sélectionné des mutants doublement bloqués et perméables au muconate pour réduire la lréquence de réversion et pour augmenter la production d'acide muconique. En expérimentation en flacons agités, on a obtenu 3.5 g/l d'acide muconique après 2 jours de fermentation. En fermentuer de 14 l, on a produit 45 g/l d'acide muconique en 4 jours de fermentation contrôlée en milieu non renouvelé à allmentation étagée. Le processus de bioconversion oxydative a également été démontré en fermenteur de 1500 l.相似文献
9.
Geraldine E. Oliva George W. Rutherford Moses Grossman Janet Shalwitz Abigail English Frances Taylor David Werdegar 《The Western journal of medicine》1988,148(5):586-589
Although adolescents account for only 0.4% of reported cases of the acquired immunodeficiency syndrome (AIDS) in the United States, they are sexually active and, therefore, at risk of acquiring human immunodeficiency virus (HIV) infection. To address issues of HIV control in adolescents, we developed guidelines that emphasize education and medical care and deemphasize antibody testing. For adolescents known to be infected with HIV, we recommend no restrictions on access to educational or treatment programs except when their health providers recommend such restrictions to protect them from exposure to opportunistic infections. For adolescents of unknown antibody status with a possible previous exposure to HIV, we recommend that as long as the incidence of HIV infection and clinical AIDS remains low, there should be no restrictions on residential placements and no routine antibody testing. 相似文献
10.
Cell-specific expression of pyruvate, pi dikinase : in situ mRNA hybridization and immunolocalization labeling of protein in wheat seed 总被引:2,自引:0,他引:2
下载免费PDF全文
![点击此处可从《Plant physiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Pyruvate, Pi dikinase (PPDK) is a key enzyme in the C4 photosynthetic pathway. However, its metabolic role in C3 plants remains uncertain. Northern blot analyses of PPDK mRNAs from wheat leaves and seeds probed with maize PPDK cDNA indicates the presence of organ-specific mRNAs. Immunofluorescent labeling of protein in wheat seed demonstrate that the PPDK polypeptide and the ribulose-1, 5-bisphosphate carboxylase small subunit polypeptide are localized predominantly in the aleurone layer and the chlorophyllous pericarp tissue, respectively. This differential distribution of the two polypeptides in wheat seed is paralleled by the differential localization of the their mRNAs as revealed by in situ hybridization. These results suggest a distinct role of cytoplasmic PPDK in seeds, which is different from the well established role in C4 photosynthesis. 相似文献