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To define structural elements critical for RNA replication in human parechovirus 1 (HPeV1), a replicon with chloramphenicol acetyltransferase as a reporter gene and an infectious virus cDNA clone have been used. It was observed that there are cis-acting signals required for HPeV1 replication located within the 5'-terminal 112 nucleotides of the genome and that these include two terminal stem-loops, SL-A and SL-B, together with a pseudoknot element. Significant disruption of any of these structures impaired both RNA replication and virus growth. In view of the similarity in terminal structures to several picornaviruses, such as cardioviruses and hepatoviruses, the insights generated in this work are of wider significance for understanding picornavirus replication. 相似文献
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Ebrahim Shokoohi Abdolrahman Mehrabi-Nasab Joaquin Abolafia Oleksandr Holovachov 《Biologia》2013,68(6):1142-1154
Three species of Plectus Bastian, 1865 viz., P. aquatilis and P. pusillus from Kerman province and P. velox from Alborz province, Iran are described and illustrated. Partial sequences of 18S region of ribosomal DNA gene were amplified for P. aquatilis and P. pusillus. The Blast results of population of P. aquatilis from Iran showed 8–10 nucleotides differences with populations of the same species (AF036602; GQ892827; AY284700) reported from the UK, Belgium and The Netherlands, respectively. Whereas Iranian population of P. pusillus showed 14–16 nucleotides differences with P. cf. pusillus (AY284705; AY284704) reported from The Netherlands. Molecular analysis revealed close relationship of the Iranian plectids with P. cf. parvus (AY284699) reported from The Netherlands. Phylogenetic relationships with other related species in the genus Plectus and closely related genera that are available in the GenBank are given. 相似文献
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Proteomic response of barley leaves to salinity 总被引:1,自引:0,他引:1
Rasoulnia A Bihamta MR Peyghambari SA Alizadeh H Rahnama A 《Molecular biology reports》2011,38(8):5055-5063
Drought and salinity stresses are adverse environmental factors that affect crop growth and yield. Proteomic analysis offers
a new approach to identify a broad spectrum of genes that are expressed in living system. We applied this technique to investigate
protein changes that were induced by salinity in barley genotypes (Hordeum vulgare L.), Afzal, as a salt-tolerant genotype and L-527, as a salt-sensitive genotype. The seeds of two genotypes were sown in
pot under controlled condition of greenhouse, using a factorial experiment based on a randomized complete block design with
three replications. Salt stress was imposed at seedling stage and leaves were collected from control and salt-stressed plant.
The Na+ and K+ concentrations in leaves changed significantly in response to short-term stress. About 850 spots were reproducibly detected
and analyzed on 2-DE gels. Of these, 117 proteins showed significant change under salinity condition in at least one of the
genotypes. Mass spectrometry analysis using MALDI-TOF/TOF led to the identification some proteins involved in several salt
responsive mechanisms which may increase plant adaptation to salt stress including higher constitutive expression level and
upregulation of antioxidant, upregulation of protein involved in signal transduction, protein biosynthesis, ATP generation
and photosynthesis. These findings may enhance our understanding of plant molecular response to salinity. 相似文献
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Karimi Ali Akbar Naghavi Mohammad Reza Peyghambari Seyed Ali Rasoulnia Abdolrahman 《In vitro cellular & developmental biology. Plant》2021,57(6):1009-1017
In Vitro Cellular & Developmental Biology - Plant - The roots of Taraxacum kok-saghyz L.E. Rodin are rich in the storage carbohydrate inulin, which is known as a probiotic compound in human... 相似文献
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Abdolrahman Amiri Rahmat Allah Fatahian Dehkordi Mohamad Saeed Heidarnejad Mohsen Jafarian Dehkordi 《Biological trace element research》2018,181(2):258-264
This research was carried out to evaluate the antidiabetic effects of zinc oxide nanoparticles (ZnO NPs) and thiamine following experimental diabetes. Fifty-six 6-week-old female mice were used and divided into seven groups of eight animals. Diabetes was induced in fasted mice by using intraperitoneal (IP) injection of alloxan (180 mg/kg). Groups included (I) non-diabetic control, (II) thiamine (30 mg/l, IP), (III) alloxan-induced diabetic mice, (IV) diabetes + ZnO NPs (0.1 mg/kg IP), (V) diabetes + ZnO NPs (0.5 mg/kg IP), (VI) diabetes + ZnO NPs (0.1 mg/kg IP) + thiamine (30 mg/l, IP), and (VII) diabetes + ZnO NPs (0.5 mg/kg IP) + thiamine (30 mg/l, IP). Coincident with pancreas recovery, in diabetic treated mice (groups IV to VII), the mean islet volume, islets per square micrometer, and volume density of the pancreas had increased than in alloxan-induced diabetic mice. ZnO NPs and thiamine induced a decreasing blood glucose, lower serum triglyceride (TG), LDL, and total cholesterol (TC) levels in alloxan-induced diabetic mice treated with ZnO NPs and thiamine, simultaneously increasing HDL as well. In conclusion, ZnO NPs and thiamine are potent antidiabetic factors, and that, these compound supplementation possesses hypoglycemic properties and have effect on serum lipid parameters in diabetes mice. 相似文献