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1.
ROWLAND-BAMFORD AMANDA J; ALLEN L HARTWELL JR; BAKER JEFFREY T; BOOTE KEN J 《Journal of experimental botany》1990,41(12):1601-1608
The atmospheric carbon dioxide (CO2) concentration has beenrising and is predicted to reach double the present concentrationsometime during the next century. The objective of this investigationwas to determine the long-term effects of different CO2 concentrationson carbohydrate status and partitioning in rice (Oryza sativaL cv. IR-30). Rice plants were grown season-long in outdoor,naturally sunlit, environmentally controlled growth chamberswith CO2 concentrations of 160, 250, 330, 500, 660, and 900µmolCO2 mol1 air. In leaf blades, the priority between the partitioningof carbon into storage carbohydrates or into export changedwith developmental stage and CO2 concentration. During vegetativegrowth, leaf sucrose and starch concentrations increased withincreasing CO2 concentration but tended to level off above 500µmolmol1 CO2. Similarly, photosynthesis also increased withCO2 concentrations up to 500µmol mol1 and thenreached a plateau at higher concentrations. The ratio of starchto sucrose concentration was positively correlated with theCO2 concentration. At maturity, increasing CO2 concentrationresulted in an increase in total non-structural carbohydrate(TNC) concentration in leaf blades, leaf sheaths and culms.Carbohydrates that were stored in vegetative plant parts beforeheading made a smaller contribution to grain dry weight at CO2concentrations below 330µmol mol1 than for treatmentsat concentrations above ambient Increasing CO2 concentrationhad no effect on the carbohydrate concentration in the grainat maturity Key words: CO2 enrichment, starch, sucrose 相似文献
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CAMILLA S. ALMEIDA PAULO F. CRISTALDO OG DESOUZA LEANDRO BACCI DANIELA F. FLORENCIO NAYARA G. CRUZ ABRAÃO A. SANTOS ALISSON S. SANTANA ALEXANDRE P. OLIVEIRA ANA P. S. LIMA ANA P. A. ARAÚJO 《Ecological Entomology》2018,43(3):371-378
1. Resource density can regulate the area that animals use. At low resource density, there is a conflict in terms of balance between costs of foraging and benefits acquired. The foraging of the higher termite Nasutitermes aff. coxipoensis consists of searching throughout trails and a building galleries phase. 2. In this study, a manipulative field experiment was used to test the hypothesis that colonies of N. aff. coxipoensis forage towards a more profitable balance between the establishment of trails and gallery construction at low resource density. 3. The experiment was conducted in north‐eastern Brazil. Seven experimental plots were established with a continuous increase in resource density (sugarcane baits). Entire colonies of N. aff. coxipoensis were transplanted from their original sites to the experimental plot, totalling 35 nests. The number, branches and total length of trails and galleries were quantified. 4. The results show that N. aff. coxipoensis optimises its foraging output, intensifying the establishment of trails at the cost of gallery construction when resource density is low. The number of trails, the number of trail branches and the total length of trails decreased with increasing resource density. Interestingly, at low resource density, the search effort was concentrated on forming longer and a greater number of trails, a small proportion of which were converted into galleries. The opposite relationship was observed at high resource density. 5. These results suggest an optimisation of search efforts during foraging depending on resource density, a mechanism that may help researchers to understand the use of space by higher termite species. 相似文献
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Identification of Immunogenic Epitopes of the 170-kDa Subunit Adhesin of Entamoeba histolytica in Patients with Invasive Amebiasis 总被引:2,自引:0,他引:2
CARLOS VELAZQUEZ IGNEZ VALETTE MIGUEL CRUZ MARIA-LUISA LABRA JULIO MONTES SAMUEL L. STANLEY Jr JESUS CALDERON 《The Journal of eukaryotic microbiology》1995,42(5):636-641
ABSTRACT. Entamoeba histolytica causes amebic dysentery (AD) and liver abscess (ALA). Little is known about protective immunity to amebiasis, and studies in this area have been complicated by the paucity of defined ameba antigens. We examined the proliferative responses of peripheral blood mononuclear cells (PBMC) from patients with AD and ALA to a recombinant protein containing a portion of the 170 kDa adhesin of E. histolytica (170CR), and to two synthetic peptides (1 and 2) derived from the 170 kDa sequence that were predicted to contain T cell epitopes. A significant number of patients with AD and ALA had PBMC that proliferated to 170CR molecule, and several individuals with ALA and AD had T cells that recognized one or both peptides. Contrarily, individuals from a non-endemic region for amebiasis did not respond to 170CR protein, or to both peptides. In regard to antibody response, nine of fifteen patients with ALA showed antibodies to 170CR protein. These same patients had antibodies to peptide 2. We identified peptides from 170-kDa adhesin that may contain both T and B cell epitopes recognized by some patients with invasive amebiasis. These peptides may be valuable reagents in studies of the immune response to amebiasis. 相似文献
6.
KARL E. HAVENS JOHN R. BEAVER THERESE L. EAST KIRSTEN WORK EDWARD J. PHLIPS MARY F. CICHRA AMANDA C. CROTEAU ANDREW J. RODUSKY ROLLAND S. FULTON III TEODORO C. ROSATI 《Freshwater Biology》2012,57(3):552-562
1. We examined 7–12 years of monthly to quarterly historical data from 15 lakes in FL, U.S.A. to determine the extent and outcome of invasion by the alien cladoceran Daphnia lumholtzi Sars. 2. The alien species was found in 10 of the 15 lakes, including Florida’s three largest lakes: Okeechobee, George and Apopka. All the surveyed lakes had resident populations of the smaller native species Daphnia ambigua Scourfield. 3. In most of the lakes, D. ambigua occurred seven to ninefold more often in plankton samples than D. lumholtzi, and at 10‐ to 100‐fold higher maximal densities. One exception was a small lake in central Florida (Lake Jesup), where D. lumholtzi attained high densities on several occasions in the 10 years of sampling. 4. In Lake Okeechobee, where data were of sufficient quality and quantity to perform statistical analyses, the results of canonical correlation analysis indicated that high densities of D. lumholtzi were correlated with lower concentrations of suspended solids, high algal biomass and higher temperature, whereas the opposite conditions were correlated with high densities of D. ambigua. 5. Based on the majority of data, D. lumholtzi has successfully invaded many lakes in Florida, yet it has not become a substantive component of the zooplankton. Additional research is needed to determine whether resources, fish predation or some other factor is responsible for this outcome of invasion. 相似文献
7.
RYAN R. KELLY LINDSAY T. MCDONALD VINCENT D. PELLEGRINI JAMES J. CRAY AMANDA C. LARUE 《Cytotherapy》2018,20(11):1371-1380
Background aims
Previous studies identified a circulating human osteoblastic population that expressed osteocalcin (OCN), increased following fracture and pubertal growth, and formed mineralized colonies in vitro and bone in vivo. A subpopulation expressed CD34, a hematopoietic/endothelial marker. These findings led to our hypothesis that hematopoietic-derived CD34+OCN+ cells exist in the circulation of mice and are modulated after fracture.Methods
Flow cytometry was used to identify CD34+OCN+ cells in male B6.SJL-PtprcaPepcb/BoyJ and Vav-Cre/mTmG (VavR) mice. Non-stabilized tibial fractures were created by three-point bend. Fractures were longitudinally imaged by micro-computed tomography, and immunofluorescent staining was used to evaluate CD34+OCN+ cells within fracture callus. AMD3100 (10 mg/kg) was injected subcutaneously for 3 days and the CD34+OCN+ population was evaluated by flow cytometry.Results
Circulating CD34+OCN+ cells were identified in mice and confirmed to be of hematopoietic origin (CD45+; Vav1+) using two mouse models. Both circulating and bone marrow-derived CD34+OCN+ cells peaked three weeks post-non-stabilized tibial fracture, suggesting association with cartilage callus transition to bone and early mineralization. Co-expression of CD34 and OCN in the fracture callus at two weeks post-fracture was observed. By three weeks, there was 2.1-fold increase in number of CD34+OCN+ cells, and these were observed throughout the fracture callus. AMD3100 altered CD34+OCN+ cell levels in peripheral blood and bone marrow.Discussion
Together, these data demonstrate a murine CD34+OCN+ circulating population that may be directly involved in fracture repair. Future studies will molecularly characterize CD34+OCN+ cells, determine mechanisms regulating their contribution, and examine if their number correlates with improved fracture healing outcomes. 相似文献8.
The reproductive interactions of the Shiny Cowbird Molothrus bonariensis , a brood parasite, and the Yellow-hooded Blackbird Agelaius icterocephalus , a host of the cowbird, were studied In Trinidad, West Indies. We gathered information on the breeding biology of the Shiny Cowbird and the Yellow-hooded Blackbird, the frequency of use of the host species, and the effects of brood parasitism on host breeding success. Yellow-hooded Blackbirds are polygynous for the most part; males build nests and attempt to attract females to lay in them by means of song and visual displays directed towards the nests. This behaviour probably makes it easy for cowbirds to locate breeding birds and their nests. Cowbird eggs were found in 153 of 377 (40–3 %) blackbird nests located before the nestling stage. Shiny Cowbird parasitism of the Yellow-hooded Blackbird had little negative impact on host reproductive success, whereas predation accounted for the majority of nest failures. Vigilant nest defense by male blackbirds combined with colonial breeding apparently also minimized the extent of host egg damage and removal by cowbirds, and the parasitized and unparasitized nests were equally successful at producing blackbirds. Cowbirds most frequently parasitized the first or only nesting attempts in blackbird territories, and first or only nests were also successful more frequently than subsequent nests. 相似文献
9.
PHILIP W. ATKINSON ALLAN J. BAKER KAREN A. BENNETT NIGEL A. CLARK JACQUIE A. CLARK KIMBERLY B. COLE ANNE DEKINGA AMANDA DEY SIMON GILLINGS PATRICIA M. GONZÁLEZ KEVIN KALASZ CLIVE D. T. MINTON JASON NEWTON LAWRENCE J. NILES THEUNIS PIERSMA ROBERT A. ROBINSON HUMPHREY P. SITTERS 《Journal of Applied Ecology》2007,44(4):885-895
10.
JESSICA E. SNODDY MARIAN LANDON GAËLLE BLANVILLAIN AMANDA SOUTHWOOD 《The Journal of wildlife management》2009,73(8):1394-1401
ABSTRACT Mortality due to fisheries interactions has been implicated as a contributor to population decline for several species of sea turtle. The incidental capture of sea turtles in the coastal gillnet fisheries of North Carolina, USA, has received much attention in recent years, and mitigation measures to reduce sea turtle mortality due to gillnet entanglement are a high priority for managers and conservationists. Efforts to evaluate effects of gillnet entanglement on sea turtle populations are complicated by the lack of information on health status of turtles released alive from nets and postrelease mortality. We obtained blood samples from green (Chelonia mydas) and Kemp's ridley (Lepidochelys kempii) sea turtles captured in gillnets for 20–240 minutes to assess the impacts of gillnet entanglement on blood biochemistry and physiological status. We measured concentrations of lactate, corticosterone, ions (Na+, K+, Cl-, P, Ca2+), enzymes (lactate dehydrogenase [LDH], creatine phosphokinase [CPK], aspartate aminotransferase [AST]), protein, and glucose in the blood and also performed physical examinations of turtles to document external indicators of health status (injuries, lethargy, muted reflexes). We evaluated the effects of entanglement time on blood biochemistry and to look for correlations between blood biochemistry and results of the physical examinations. We observed a significant increase in blood lactate, LDH, CPK, phosphorus, and glucose with increased entanglement time. Alterations in blood biochemistry were generally associated with a decline in health status as indicated by results of the physical examination. Although entanglement time plays an important role in determining the health status of sea turtles upon release from a gillnet, our results suggest that factors such as the depth and severity of entanglement may also have an effect on health status of turtles and the probability of postrelease survival. We were unable to set a maximum unattended gillnet soak time to minimize impacts on captured sea turtles, and therefore recommend that fisheries managers continue to enforce the net attendance regulations currently in place in the lower Cape Fear River, North Carolina, during the summer months. 相似文献