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Tolerance in banana to Fusarium wilt is associated with early up-regulation of cell wall-strengthening genes in the roots 总被引:1,自引:0,他引:1
NOËLANI VAN DEN BERG DAVE K. BERGER INGO HEIN PAUL R. J. BIRCH MICHAEL J. WINGFIELD ALTUS VILJOEN 《Molecular Plant Pathology》2007,8(3):333-341
Fusarium wilt, caused by the fungal pathogen Fusarium oxysporum f. sp. cubense ( Foc ), is one of the most destructive diseases of bananas. In the tropics and subtropics, Cavendish banana varieties are highly susceptible to Foc race 4 (VCG 0120). Cavendish selection GCTCV-218 was shown to have significantly lower disease severity and incidence compared with susceptible cultivar Williams in replicated greenhouse and field trials. Suppression subtractive hybridization (SSH) was previously carried out to identify genes induced in roots of GCTCV-218, but not in Williams, after infection with Foc 'subtropical' race 4 . Seventy-nine SSH clones were sequenced and revealed 13 non-redundant gene fragments, several of which showed homology to defence-associated genes, including cell wall-strengthening genes. Quantitative RT-PCR was used to confirm up-regulation and differential expression of a number of genes throughout a time-course, following Foc infection in the tolerant GCTCV-218 when compared with susceptible cv. Williams . Tolerance of GCTCV-218 was linked to significantly increased induction of cell wall-associated phenolic compounds. 相似文献
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Abstract. An improved method is described for micro-autoradiography of water-soluble substances alter freeze-substitution of plant tissue in which water is rigorously excluded. Resin sections are cut and flattened dry, and dry photographic emulsion is mounted on them. When the location of 14 C in wheat leaves after assimilation of 14 CO2 was studied with this method, it was found that 14 C entered the intermediate veins before the laterals and entered both types of veins along the flanks of the veins adjacent to the phloem. High concentrations of 14 C were found in small spaces in the cells of mesophyll and vein parenchyma; these spaces coincide with the nuclei. The concentration of 14 C in these nucleus-associated spaces was as high as that reached at later times in the sieve tubes. Water washed the 14 C out of these spaces of the sections and the label in the washings was predominantly in sucrose. The high 14 C concentrations of the nucleus-associated spaces were particularly easily leached. It is concluded that the raising of the sugar concentration to the high levels found in sieve tubes can take place in these leaves in a special space in each mesophyll cell not, or not only, at the boundary of the sieve tubes. 相似文献
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