全文获取类型
收费全文 | 408篇 |
免费 | 48篇 |
国内免费 | 1篇 |
出版年
2022年 | 5篇 |
2021年 | 15篇 |
2020年 | 5篇 |
2019年 | 5篇 |
2018年 | 10篇 |
2017年 | 6篇 |
2016年 | 5篇 |
2015年 | 14篇 |
2014年 | 18篇 |
2013年 | 20篇 |
2012年 | 29篇 |
2011年 | 25篇 |
2010年 | 15篇 |
2009年 | 17篇 |
2008年 | 21篇 |
2007年 | 17篇 |
2006年 | 12篇 |
2005年 | 16篇 |
2004年 | 7篇 |
2003年 | 12篇 |
2002年 | 15篇 |
2001年 | 8篇 |
2000年 | 11篇 |
1999年 | 10篇 |
1998年 | 11篇 |
1997年 | 3篇 |
1996年 | 7篇 |
1995年 | 5篇 |
1994年 | 7篇 |
1992年 | 6篇 |
1991年 | 3篇 |
1989年 | 5篇 |
1988年 | 6篇 |
1987年 | 7篇 |
1986年 | 4篇 |
1985年 | 4篇 |
1984年 | 3篇 |
1983年 | 7篇 |
1982年 | 4篇 |
1981年 | 4篇 |
1976年 | 3篇 |
1975年 | 9篇 |
1974年 | 4篇 |
1973年 | 3篇 |
1972年 | 2篇 |
1971年 | 8篇 |
1970年 | 2篇 |
1969年 | 4篇 |
1968年 | 4篇 |
1967年 | 2篇 |
排序方式: 共有457条查询结果,搜索用时 15 毫秒
1.
Gene flow between populations of two invertebrates in springs 总被引:2,自引:0,他引:2
1. Using allozymes, we analysed genetic structure of the freshwater gastropod Bythinella dunkeri and the freshwater flatworm Crenobia alpina. The two species are habitat specialists, living almost exclusively in springs. The sampled area in Hesse (Germany) covers a spatial scale of 20 km and includes two river drainages. From the biology of the two species we expected little dispersal along rivers. However, the possibility exists that groundwater provide suitable pathways for dispersal. 2. In B. dunkeri heterozygosity decreased from west to east. For some alleles we found clines in this geographic direction. These clines generated a positive correlation between geographic distance and genetic differentiation. Furthermore patterns of genetic variation within populations suggested that populations may have been faced with bottlenecks and founder effects. If populations are not in population genetic equilibrium, such founder effects would also explain the rather high amount of genetic differentiation between populations (10%). 3. For C. alpina the mean number of alleles decreased with increasing isolation of populations. Genetic differentiation between populations contributed 19% to the total genetic variation. Genetic differentiation was not correlated to geographic distance, but compared with B. dunkeri variability of pairwise differentiation between pairs of populations was higher in C. alpina. 4. Overall B. dunkeri appears to be a fairly good disperser, which may use groundwater as dispersal pathway. Furthermore populations seem to be not in equilibrium. In contrast C. alpina forms rather isolated populations with little dispersal between springs and groundwater seems to play no important role for dispersal. 相似文献
2.
Kinetics of Butyrate, Acetate, and Hydrogen Metabolism in a Thermophilic, Anaerobic, Butyrate-Degrading Triculture 总被引:7,自引:4,他引:3
下载免费PDF全文
![点击此处可从《Applied microbiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Kinetics of butyrate, acetate, and hydrogen metabolism were determined with butyrate-limited, chemostat-grown tricultures of a thermophilic butyrate-utilizing bacterium together with Methanobacterium thermoautotrophicum and the TAM organism, a thermophilic acetate-utilizing methanogenic rod. Kinetic parameters were determined from progress curves fitted to the integrated form of the Michaelis-Menten equation. The apparent half-saturation constants, Km, for butyrate, acetate, and dissolved hydrogen were 76 μM, 0.4 mM, and 8.5 μM, respectively. Butyrate and hydrogen were metabolized to a concentration of less than 1 μM, whereas acetate uptake usually ceased at a concentration of 25 to 75 μM, indicating a threshold level for acetate uptake. No significant differences in Km values for butyrate degradation were found between chemostat- and batch-grown tricultures, although the maximum growth rate was somewhat higher in the batch cultures in which the medium was supplemented with yeast extract. Acetate utilization was found to be the rate-limiting reaction for complete degradation of butyrate to methane and carbon dioxide in continuous culture. Increasing the dilution rate resulted in a gradual accumulation of acetate. The results explain the low concentrations of butyrate and hydrogen normally found during anaerobic digestion and the observation that acetate is the first volatile fatty acid to accumulate upon a decrease in retention time or increase in organic loading of a digestor. 相似文献
3.
Haemogregarina georgianae n. sp. occurs frequently in the bathydraconid teleost Parachaenichthys georgianus from the western Antarctic portion of the Southern Ocean. The haemogregarine and its developmental stages in the vertebrate have been found in erythrocytes of the fish: both microschizogony and macroschizogony have been seen in fish caught during the austral summer. Morphological evidence suggests the merozoites from macroschizogony give rise to the microschizont, and that the microschizont merozoites give rise to gametocytes. Comparison of H. georgianae with other haemogregarines known from teleosts shows that it has a previously undescribed morphology. 相似文献
4.
Chromosomal protein HMG-14. Complete human cDNA sequence and evidence for a multigene family 总被引:7,自引:0,他引:7
D Landsman T Srikantha R Westermann M Bustin 《The Journal of biological chemistry》1986,261(34):16082-16086
5.
6.
7.
It is unlikely that the intact or commonly preserved varieties of Ordovician-Silurian nautiloid shells were able to drift for any distance at the surface of the sea even if they died there. Their cameral capacity was much larger than the volume of the extracted or decayed body, and it would have contained a partial vacuum and cameral liquid when they were alive. The closely spaced and thin septa of the shallow-water adapted species were liable to buckle in compression and then implode in local tension during reverse hydrostatic loading by water pressure. This reverse loading and internal implosion of the septa was probably initiated by the sudden cameral refilling of an apical chamber caused by the depositional rupture of the apical siphuncle at or near the maximum habitat depth of these species. The instantaneous buckling of the more adorai septa was potentially terminated by variations in the septum thickness and cameral fill-fractions at that time, and they imply that some of the Silurian nautiloids from Bohemia were deposited at a minimum depth of about 65 m. Alternative interpretations involving the breakage of the same septa in tension, or buckling due to the difference in pressure between adjacent flooded chambers, set a maximum depth limit of about 160 m for the same facies. Many of the smaller Silurian nautiloids were unlikely to buckle during refilling, and they were potentially flooded faster than they could sink, below a depth of 100–300 m. 相似文献
8.
Role of the mitochondrial DnaJ homolog Mdj1p as a chaperone for mitochondrially synthesized and imported proteins. 总被引:3,自引:1,他引:2
下载免费PDF全文
![点击此处可从《Molecular and cellular biology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
B Westermann B Gaume J M Herrmann W Neupert E Schwarz 《Molecular and cellular biology》1996,16(12):7063-7071
Mdj1p, a DnaJ homolog in the mitochondria of Saccharomyces cerevisiae, is involved in the folding of proteins in the mitochondrial matrix. In this capacity, Mdj1p cooperates with mitochondrial Hsp70 (mt-Hsp70). Here, we analyzed the role of Mdj1p as a chaperone for newly synthesized proteins encoded by mitochondrial DNA and for nucleus-encoded proteins as they enter the mitochondrial matrix. A series of conditional mutants of mdj1 was constructed. Mutations in the various functional domains led to a partial loss of Mdj1p function. The mutant Mdj1 proteins were defective in protecting the tester protein firefly luciferase against heat-induced aggregation in isolated mitochondria. The mitochondrially encoded var1 protein showed enhanced aggregation after synthesis in mdj1 mutant mitochondria. Mdj1p and mt-Hsp70 were found in a complex with nascent polypeptide chains on mitochondrial ribosomes. Mdj1p was not found to interact with translocation intermediates of imported proteins spanning the two membranes and exposing short segments into the matrix, in accordance with the lack of requirement of Mdj1p in the mt-Hsp70-mediated protein import into mitochondria. On the other hand, precursor proteins in transit which had further entered the matrix were found in a complex with Mdj1p. Our results suggest that Mdj1p together with mt-Hsp70 plays an important role as a chaperone for mitochondrially synthesized polypeptide chains emerging from the ribosome and for translocating proteins at a late import step. 相似文献
9.
Direct sequencing of the mitochondrial displacement loop (D-loop) of shrews
(genus Sorex) for the region between the tRNA(Pro) and the conserved
sequence block-F revealed variable numbers of 79-bp tandem repeats. These
repeats were found in all 19 individuals sequenced, representing three
subspecies and one closely related species of the masked shrew group (Sorex
cinereus cinereus, S. c. miscix, S. c. acadicus, and S. haydeni) and an
outgroup, the pygmy shrew (S. hoyi). Each specimen also possessed an
adjacent 76-bp imperfect copy of the tandem repeats. One individual was
heteroplasmic for length variants consisting of five and seven copies of
the 79-bp tandem repeat. The sequence of the repeats is conducive to the
formation of secondary structure. A termination-associated sequence is
present in each of the repeats and in a unique sequence region 5' to the
tandem array as well. Mean genetic distance between the masked shrew taxa
and the pygmy shrew was calculated separately for the unique sequence
region, one of the tandem repeats, the imperfect repeat, and these three
regions combined. The unique sequence region evolved more rapidly than the
tandem repeats or the imperfect repeat. The small genetic distance between
pairs of tandem repeats within an individual is consistent with a model of
concerted evolution. Repeats are apparently duplicated and lost at a high
rate, which tends to homogenize the tandem array. The rate of D- loop
sequence divergence between the masked and pygmy shrews is estimated to be
15%-20%/Myr, the highest rate observed in D-loops of mammals. Rapid
sequence evolution in shrews may be due either to their high metabolic rate
and short generation time or to the presence of variable numbers of tandem
repeats.
相似文献
10.