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利用热变性温度法测得球壳孢目5属14种19个菌株DNAG+Cmol%的变化范围为48.08~53.7平均值为51.12,与文献报道的丝孢菌类的平均值接近,也与子囊菌的平均值相差不大,提示该类群的种类及其与大多数丝孢菌间在系统演化上似具有一定的同源性,与形态分类对半知菌的处理相吻合,也为其有性阶段大多属子囊菌这一事实提供了佐证;各属,种GC值接近,且相互璋有交叉,种内菌株间差异小于2%,方差分析显示 相似文献
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InverstigationofthediseasesofcultivthedRosa‘hybrida’InagreenhousenearthecampusofShenyangAgriculturalUniversityinNovember,1996hasresultedinthediscoveryofaperonosporaspeciespreviouslyunrecordedinChina.Thefunguscausedseriousdamagetosome2000roseplants.ItwasidentifiedasPeronosporaSparsaBerk.exBerk.&Broome.peronosporasparsaBerk.exBerk.exBerk.&Broome;Gard.ChronSer.1.14:308,1862;Saccardo.Syll.Fung.7:263,188aDelowerfeatesnn13t)tsealing山sCdbmdiemons,leafspotslrregulapchlorosls,lat… 相似文献
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The genus Papulaspora was named by Preuss in 1851 based on the characters of “bulbils” (papulospores) and interpreted by Hotson (1912,1917) as consisting of sterile mycelia with various kinds of “bulbils”. 25 Papulaspora species were described by Hotson in detail (1912, 1917), among them 10 species were acknowledged by Weresub & LeClair (1971) and 20 species were recorded in the Dictionary of the Fungi (2003). The ecology of Papulaspora species is not well known, most species were isolated from dung, decaying plant material or soil. 相似文献
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东北地区保护地土壤真菌多样性研究Ⅰ 总被引:1,自引:1,他引:0
2003年4月至2004年7月在辽宁、吉林、黑龙江三省的20多个市县共采集不同年限的保护地土样150多份,采用稀释平板法在孟加拉红培养基上分离近千个真菌菌株,通过纯化培养和形态鉴定,将各菌株鉴定到种。本文报道了部分可培养的子囊菌和有丝分裂孢子真菌的研究结果,其中子囊菌5属6种,包括两个中国新记录种(Lophotrichus brevirostratus和Microascus cinereus)和一个国内记录但未正式发表的子襄菌(Emericellopsis terricola);有丝分裂孢子真菌7属10种。 相似文献
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Twenty species of Aspergillus and their teleomorphs were isolated from naturally fermented soybean pastes collected in 42 regions of China. Of which A. tubingensis, A. flavus, A. niger, A. ochraceus, A. candidus and A. fumigatus were predominant. The high frequency of A. flavus from the samples indicates that the traditional making method of soybean paste might possibly be harmful to food safety. Some Aspergillus species or strains isolated from the traditional food samples may be hopeful of using in pure leaven making and industrial production of soybean paste with special flavor. 相似文献
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枣树干腐病病原菌的鉴定 总被引:4,自引:0,他引:4
对山西省枣树上发生的一种新病害进行了调查和病菌分离与培养研究。通过形态学特征观察和rDNA ITS序列分析,证明该病菌为葡萄座腔菌Botryosphaeria dothidea的无性阶段Fusicoccum aesculi。 相似文献
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以苍耳柄锈菌三裂叶豚草专化型Puccinia xanthii f. sp. ambrosiae-trifidae为研究试材,比较研究了其冬孢子DNA提取的9种方法,其中CTAB-钢珠法、改良的微型电钻法以及EZ-Kit改良法获得的基因组DNA经检测质量较好。在此基础上,利用ITS-PCR和ISSR引物UBC#835将待用DNA进行PCR扩增检测。结果表明,上述3种方法提取的DNA适合于ISSR反应。研究结果为专性寄生锈菌分子遗传变异的研究提供了保障。 相似文献
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采用AGI-30生物采样器收集鸡舍空气样本,同时采集鸡舍中饲料、积尘、土壤和饮用水在内的环境基质样品。采用形态学方法对分离获得的镰孢菌菌株进行鉴定,利用tri5-PCR技术对镰孢菌菌株中产单端孢霉烯族毒素的菌株进行检测,目的是探明鸡舍环境中镰孢菌种类的分布特征和产毒菌株。结果表明,从采集的50份样品中分离获得139个镰孢菌菌株,鸡舍空气和基质中的优势菌株均为Fusarium verticillioides;在各基质中,土壤中镰孢菌总浓度最高,为4×102–1.35×104CFU/g,其次为饲料和饮用水;采用tri5-PCR技术筛选到42株tri5阳性镰孢菌菌株,其中以F. graminearum所占比例最高。研究明确鸡舍中镰孢菌种类及其分布特征对鸡只疾病控制及保障人类和动物的健康具有重要意义。 相似文献