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大鼠脑室注射高渗氯化钠和蔗糖诱导饮水行为和脑内c—fos的表达 总被引:2,自引:0,他引:2
用SD种系清醒大鼠,观察脑室注射高渗物质引起的饮水及c-fos在脑内的表达部位。实验结果表明,脑室内微量注射1.5mol/L、3mol/L NaCl或3mol/L蔗糖均可诱导饮水反应,并在前脑的终板血管器官、正中视前核和下丘脑视上核与室旁核中见到Fos样免疫反应阳性细胞,同样在后脑的最后区、臂旁外侧核与孤束核中也能见到Fos样免疫反应阳性细胞,同样在后脑的最后区、臂旁外侧核与孤束核中也能见到Fos 相似文献
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神经干细胞/前体细胞分化的功能学鉴定研究进展 总被引:1,自引:0,他引:1
神经干细胞/前体细胞分化是神经生物学研究的热点.以往对神经干细胞/前体细胞分化水平的鉴定主要依赖于形态学指标,而随着膜片钳技术的应用,神经干细胞/前体细胞分化过程中膜电特性的改变、离子通道活动等功能学指标越来越受到重视.综述了利用膜片钳技术研究神经干细胞/前体细胞功能分化的最新进展,并对存在的问题做出思考和展望. 相似文献
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弧束核参与剌激下丘脑室旁核的镇痛作用 总被引:4,自引:1,他引:3
This study was undertaken to evaluate the analgesic effect of paraventricular nucleus (PVN) stimulation with tail stimulation-vocalization test. The mechanism of this analgesia was analysed with nuclear lesion and microinjection technique. The main results were as follows: (1) Electrical stimulation of the PVN could significantly enhance the pain threshold and increase the content of AVP in brainstem measured by radioimmunoassay. (2) Solitary tract nucleus (STN) lesion could eliminate the analgesic effect induced by PVN stimulation. (3) Intranuclear microinjection of AVP-antagonist and AVP-antiserum into the STN could block the analgesic effect of PVN stimulation. (4) Intranuclear microinjection of AVP into the STN could mimick the analgesic effect similar to that of PVN stimulation. These results suggest that electrical stimulation of the PVN could produce an analgesic effect. This effect might be mediated by the activation of VP-ergic neurons in PVN and upon releasing VP from the descending fibers, the activities of neurons in the STN are influenced. 相似文献
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孤束核参与刺激下丘脑室旁核的镇痛作用 总被引:1,自引:0,他引:1
本实验用电刺激鼠尾-嘶叫法测痛,观察电刺激下丘脑室旁核的镇痛效应,并采用核团损毁和核团内微量注射药物等方法分析其镇痛通路。实验结果如下:(1)电刺激下丘脑室旁核能产生明显的镇痛效应。同时,放射免疫测定发现脑干加压素含量升高。(2)损毁孤束核能取消刺激下丘脑室旁核的镇痛效应,但对基础痛阈无影响。(3)孤束核内微量注射加压素拮抗剂[d(CH_2)_5 TYr(Me)-AVP]60ng/0.6μl 和加压素抗血清0.6μl 都可明显对抗刺激下丘脑室旁核的镇痛效应。(4)直接在孤束核内微量注射加压素60ng/0.6μl,能模拟刺激下丘脑室旁核的镇痛效应。实验结果表明:电刺激下丘脑室旁核能产生镇痛效应,其机理之一可能是兴奋了下丘脑室旁核中加压素能神经元胞体,后者通过下行投射纤维在孤束核中释放加压素,影响孤束核神经元的活动,从而产生镇痛。 相似文献
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