枸杞组织培养中形态发生与核酸蛋白质合成动态的研究

应用放射自显影技术观察枸杞叶片组织培养中形态发生与核酸、蛋白质合成动态之间的关系。结果表明,培养初期,先以外层少数叶肉细胞开始RNA和蛋白质的合成,接着DNA也开始合成。在分生细胞团及愈伤组织形成过程中,三种大分子的合成一直处于相当活跃的状态。当愈伤组织分化为芽时,DNA和RNA的合成明显下降,而蛋白质的合成还很旺盛.     

皖南汉族的趾纹研究

本文对皖南地区520例汉族大学生的趾纹进行了调查分析,计算出了各型趾纹频率、趾纹组合、趾嵴纹计数等项参数。比较了不同性别、不同侧别和不同人种间的差异,分析了趾纹类型和趾嵴纹计数在各趾的分布特点,并对差异的原因进行了讨论。Abstract: The authors inverstigated and analysed the toe prints of 520 cases of good healthy students in Wannan area of Aahui, and calculated the frequency of various toe patterns, combinations of toe patterns, toe ridge counts (TRC) and so on. The differences between sexes, sides and reces were compared. The distributive character of toe patterns and toe ridge count on every toe were analysed. The different cause were discussed.     

小麦体细胞胚发生的超微结构研究

对小麦（Ｔｒｉｔｉｃｕｍ ａｅｓｔｉｖｕｍ Ｌ．）幼胚培养中体细胞胚发生过程的超微结构变化进行了研究,结果如下：（１）原体细胞中大液泡消失,存在大量小液泡,细胞质的电子密度加强,核大,核仁明显,出现多核仁;（２）细胞器数量和种类,如质体、核糖体和线粒体增加;（３）细胞壁加厚,胞间连丝逐渐消失,细胞器数量丰富,胚性细胞中积累淀粉;（４）细胞壁加厚的胚性细胞中存在核仁液泡、自体吞噬泡和分泌泡;（５）多细胞原胚、球形胚和梨形胚被一层外壁包围,但胚体内细胞间广泛存在胞间连丝;（６）成熟胚生长点部位的细胞内质体中出现膜系结构,已向叶绿体转变,类囊体已形成     

Call Characteristic Network Reveal Geographical Patterns of Call Similarity:Applying Network Analysis to Frog’s Call Research

Individual’s phenotypic traits are the results of adaptation to ecological conditions.Therefore,different selection pressures caused by heterogeneous environments may result in phenotypic difference,especially for individuals in different geographical populations.Here,we illustrated for the first time to use social network analysis(SNA)for examining whether geographical proximity predicts the similarity patterns in call characteristics among populations of an anuran species.We recorded calls from 150 male dorsal-striped opposite-fingered treefrogs(Chiromantis doriae)at 11 populations in Hainan Province and one population in Guangdong Province in China's Mainland,and we measured eight acoustic variables for each male.Mantel test didn’t show a correlation between geographical proximity and the similarity in call characteristics among populations.In addition,we failed to find correlations between a population’s eigenvector centrality and the distance to its nearest neighbor,nor between the coefficient of variation of similarity in call characteristics of a population and the average distance to all other populations.Nevertheless,three acoustic clusters were identified by the Girvan-Newman algorithm,and clustering was partially associated with geography.Furthermore,the most central populations were included in the same cluster,but the top betweenness populations were located within different clusters,suggesting that centrality populations are not necessary bridging between clusters.These results demonstrate the potential usefulness of the SNA toolbox and indicate that SNA helps to uncover the patterns that often overlooked in other analytical methods.By using SNA in frog’s call studies,researchers could further uncover the potential relationship in call characteristics between geographical populations,further reveal the effects of ecological factors on call characteristics,and probably enhance our understanding of the adaptive evolution of acoustic signals.     

人多肽链延伸因子1α四个反转录假基因的鉴别与定位

人多肽链延伸因子1α基因EEF1A是一个在蛋白质合成过程中起重要作用的看家基因。通过对已有GenBank、GDB等数据库的综合分析,鉴别了4个人类多肽链延伸因子1α基因的反转录假基因,并分别将其精细定位于4q2 5、7p15-21、9q34和19q13上。 Abstract:The gene for human polypeptide chain elongation factor-1α(EEF1A)is a house-keeping gene which plays an important role in the process of protein synthesis.By means of comprehensive analysis in the database of Genbank,GDB and ect,we identify 4 retropseudogenes of EEF1A and finely localized to 4q25,7p15-21,9q34 and 19q13,respectively.     

植物体细胞胚发生中ATP酶活性时空分布动态与内源激素的变化

本文以我们的研究结果为基础,并结合国内外近几年有关研究报道,对植物体细胞胚发生中的超微结构和ATP酶活性时空分布动态及内源激素的变化和作用进行专题评述。⑴ 超微结构的变化：当植物体细胞一旦转化为胚性细胞后,各种细胞器相继增加,不仅丰富而且活跃,特别是线粒体内嵴发达,有的正处于分裂状态;核糖体聚集成多聚核糖体;质体中含大量淀粉粒,接着出现高尔基体等。早期胚性细胞与周围细胞还存在胞间连丝,随着胚性细胞壁的加厚,胞间连丝也随之消失。⑵ ATP酶时空分布动态：早期的胚性细胞中ATP酶反应产物主要沉积于质膜和液泡膜上,后期ATP酶活性转入细胞内,液泡和细胞核中,而且在胚性细胞壁加厚处有活跃的ATP酶活性反应,并证明ATP酶活性是在胚性细胞发生过程中形成的。⑶ 内源激素的变化与作用：在体细胞胚诱导过程中内源激素起着关键性作用,内源生长素含量的提高为胚性细胞的诱导奠定了基础,细胞分裂素含量的增加可促进胚性细胞的分裂和增殖,ABA不仅提高了体细胞胚的诱导频率,而且促进了体细胞胚的正常发育。     

不同土壤水分条件下CO2浓度对禾谷缢管蚜种群的影响

利用开顶式熏气室研究了不同土壤水分条件下不同CO2浓度对禾谷缢管蚜种群的影响,以期对未来大气CO2浓度升高条件下不同降雨地区的小麦－蚜虫关系发展趋势做出蚜虫关系发展趋势做出初步预测,结果表明,随CO2浓度从350ul.L^-1上升至550ul.L^-1时,60％土壤水分下的种群增长最快;当CO2浓度从550ul.L^-1上升到700ul.L^-1时,60％和40％土亍水分下的种群增长相近,且高于80％土壤水分下的增长,据此可以认为,随大气CO2浓度升高,禾谷缢管蚜种群会持续增长,从目前至下世纪中叶的时间内可能是蚜种群增长最快的阶段,特别在干旱,半干旱地区禾谷缢管蚜种群增长幅度较大,小麦受受较重。