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小麦背景中黑麦1R染色体的遗传变异 总被引:8,自引:0,他引:8
运用细胞遗传学方法鉴定了来源于中国春×M27(1R/1D代换系)的花粉植株和F2单株染色体组成。发现7个花粉植株中出现7种染色体组成变异类型,每株呈现一类变异;而27个F2单株中,存在11种染色体组成变异类型,变异频率仅为37.0%,低于花粉植株。花粉植株群体中,观察到一个能稳定向后代传递的小麦/1R小片段易位,但F2群体中未检测到小麦/黑麦易位。表明常规染色体工程结合花药培养是有计划、有目的实现异源染色体小片段向小麦转移的简便、高效、快速途径。 相似文献
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黑麦1R染色体特异性PCR引物的分子证据 总被引:3,自引:0,他引:3
Based on the differences of rRNA intergenic sequences between wheat ( Triticum aestivum L. ) and rye ( Secale cereale L. ), rye specific primer set NOR-R1 was synthesized according to Koebner' design. PCR analyses were carried out on different DNA substrates of common wheat and its relatives such as Agropyron elongataum (Host) Beauv., Haynaldia villosa Shur. and Hordeum vulgare L. The results confirmed that NOR-R1 primer set is specific to rye. It was found that PCR using DNAs from wheat materials containing 1R chromosome resulted in the specific amplification products of rye, whereas no amplification product was detected in PCR when using DNAs with other rye chromosomes. FISH (Fluorescent in situ Hybridization) further revealed that the binding sites for the primer set NOR-R1 were only on nucleolar organizing region of chromosome 1R. These results indicated that the primer set NOR-R1 provides a useful means for molecular tagging of rye chromosomes 1 R in wheat genetic background. 相似文献
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黑麦1R染色体特异性PCR引物的分子证据 总被引:10,自引:0,他引:10
根据黑麦(SecaleceraleL.)与小麦(TriticumaestwumL.)rRNA基因间隔区序列差异,按Koebner设计的引物序列,合成了黑麦特异引物NOR-R1。运用该引物对不同植物材料进行PCR扩增,观察表明,含有黑麦1R染色体的植物均扩增出黑麦的特异带,但含有其他黑麦染色体的小麦种质,普通小麦品种及其近缘物种长穗偃麦草(Agropyronelongatum(Host)Beauv) 相似文献
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