马铃薯Y病毒N株HC-pro基因的序列分析与表达

The HC-pro gene was amplified by RT-PCR from total RNA of tobacco leaves infected with a N strain of Potato virus Y in Shaanxi, and cloned into the PMD 18-T vector. This HC-pro gene is consisted of 1371 nucleotides, encoding 457 amino acids. It shared the sequence homologyof 82.5%-96.4% nucleotide acid and 92.5%-98.0% in amino acids compared to 9 species of PVY N HC-pro abroad. The HC-pro gene was inserted into prokaryotic expressing vector pBV221, to obtain pBVHC recombinant plasmid in E. coli BL21. SDS-PAGE indicated that HC-pro proteins are successfully expressed in E. coli, Western blotting analysis demonstrated that the antibody against the expressed HC-pro can be used to identify the infected plants .     

马铃薯Y病毒N株HC-pro基因的序列分析与表达

马铃薯Ｙ病毒(Potato virus Y,PVY)是马铃薯Ｙ病毒属(Potyvirus)的典型成员,是烟草、马铃薯和辣椒等茄科作物病毒病的主要病原之一,分布于世界各地.近年烟草上的 PVY在全国范围内特别是陕西、黄淮和东北烟区流行呈上升趋势,且以PVY坏死株系（PVY-N)为主,重病田发生率达40%以上,并常与烟草花叶病毒、黄瓜花叶病毒混合侵染,严重影响烟草品质造成巨大的经济损失.