不同形式混合资料遗传力的估计方法

本文探讨了用一种特殊形式资料估计遗传力的方法,亦可作为为扩大样本含量,充分利用不同来源且形式不同资料合并估计遗传力的方法。     

Activation of STAT3 stimulates AHSP expression in K562 cells

Studies on the chaperone proteinα-hemoglobin stabilizing protein(AHSP)reveal that abundant AHSP in erythroid cells enhance the cells’tolerance to oxidative stress imposed by excessα-hemoglobin in pathological conditions.However,the potential intracellular modulation of AHSP expression itself in response to oxidative stress is still unknown.The present study examined the effect and molecular mechanism of STAT3,an oxidative regulator,on the expression of AHSP.AHSP expression increased in K562 cells upon cytokine IL-6-induced STAT3 activation and decreased in STAT3 knock-down K562 cells.Regulation of AHSP in oxidative circumstance was then examined inα-globin-overloaded K562 cells,and real-time PCR showed strengthened expression of both AHSP and STAT3.ChIP analysis showed binding of STAT3 to AHSP promoter and binding was significantly augmented with IL6 stimulation and uponα-globin overexpression.Dual luciferase reporter assays of the wildtype and mutated SB3 element,an IL-6RE site,in the AHSP promoter in K562 cells highlighted the direct regulatory effect of STAT3 on AHSP gene.Finally,direct binding of STAT3 to SB3 site of AHSP promoter was confirmed with EMSA assays.Our work reveals an adaptive AHSP regulation mediated by the redox-sensitive STAT3 signaling pathway,and provides clues to the therapeutic strategy for AHSP enhancement.     

In vitro Anti-viral Activity of the Total Alkaloids from Tripterygium hypoglaucum against Herpes Simplex Virus Type 1

Herpes simplex virus type 1 (HSV-1) is a commonly occurring human pathogen worldwide. There is an urgent need to discover and develop new alternative agents for the management of HSV-1 infection. Tripterygium hypoglaucum (level) Hutch (Celastraceae) is a traditional Chinese medicine plant with many pharmacological activities such as anti-inflammation, anti-tumor and antifertility. The usual medicinal part is the roots which contain about a 1% yield of alkaloids. A crude total alkaloids extract was prepared ...     

A Stable Upstream Stem-loop Structure Enhances Selection of the First 5'-ORF-AUG as a Main Start Codon for Translation Initiation of Human ACAT1 mRNA

Acyl-coenzyme A:cholesterol acyltransferase (ACAT)is an integral membrane protein, which is mainly locatedin rough endoplasmic reticulum (ER), and is responsiblefor catalyzing the intracellular formation of cholesterylester from cholesterol and long-chain fatty acyl-coenzymeA [1,2]. Human ACAT1 cDNA K1 was firstly cloned andfunctionally expressed in 1993 [3]. Further studies withspecific anti-ACAT1 antibody (DM10) illustrated that onemajor 50 kD ACAT1 protein was expressed in various…     

THE EARLY MIDDLE DEVONIAN BRYOZOANS FROM WUTSUN SHALE,KWANGSI.

INTRODUCTIONThe material dealt with in this paper came from the upper part of the Wu-tsun shale near Liuchingyu.The village is located on the right bank of Yukiang,about 12 km.to the north of Yungchun city.The Wutsun shale consists mainlyof shales and sandstones with a bed of argillaceous limestone in the upper partvarying from a few to 15 meters thick.The bryozoans occur in the uppermostpart of the formation,where the rock contains high iron contents.They are     

RFLP诊断方法的改进

RFLP(限制性片段长度多态)技术应用于遗传病的诊断始于1981年。在十年时间里,这一基因诊断技术和分析方法不断得到发展和改进。随着越来越多的致病基因和连锁DNA多态片段的分离和克隆,该技术在分析缺陷基因性质,携带者检出和产前诊断等方面将发挥重要作用.然而,RFLP技术复杂,耗费时间和费用较大,使其向临床推广应用面临很大局限性。因此,基因诊断的方法简化和技术改进大有必要。1985年首创的多聚酶链     

MIDDLE ORDOVICIAN MOLLUSCS FROM THE PAIYANGHO REGION,CHILIENSHAN(“NANSHAN”)

INTRODUCTIONThis paper deals with all the fossil molluscs collected respectively by Dr.P.Li's party in1956 and Dr.T.H.Yin's party in 1957 of the Institute of Geology,Academia Sinica.Pre-liminary reports regarding the discovery of the Ordovician in the Paiyangho region has been madeby Li,and Yang(1957),and Li,Hsieh and Ho(1958),while the writer had twice reported     

更正信

Dear Editors, I am the first author of the paper “YANG Xia, LIU Ran. Intraganglionic laminar endings act as mechanoreceptors of vagal afferent nerve in guinea pig esophagus. Acta Physiol Sin 2006; 58（2）： 171-176”. I would like to ask for a correction to be published on the journal concerning the authorship; I should take responsibility for the previous incorrect listing and I am sorry for the trouble brought to the editors and readers.[第一段]     

降低PARP酶活性对外源基因稳定整合的影响

通过使用 PARP酶的抑制剂研究了降低PARP酶的活性对外源基因转染效率及整合作用的影响, 结果表明,转染的外源基因的吸收及瞬时表达不依赖于PARP酶的活性,而外源基因的整合作用与PARP酶的活性有关。 Abstract:In this study,the effect of lowering PARP activity on the transfection of cultured cells with foreigh genes was evaluated.The results indicated that PARP enzyme involved in the stable integration of foreign genes into the host genome.However,inhibition of PARP activity exhibited no effect on both the uptake into the cells and the expression of the forging genes.