恒河猴(Macaca mulatta)转移因子的研究 Ⅰ转移对结素和破类延迟超敏反应到人的实验研究

将对结素和破类呈现阳性皮肤延迟超敏反应的恒河猴的淋巴结和脾脏剪碎,匀浆,过滤和高速离心,以葡聚糖G25凝胶过滤分离成6个组分。只有组分Ⅳ不仅能将皮肤延迟超敏反应在同种异体之间转移,而且能从恒河猴转移到人。不管TF供体和培养淋巴细胞供体对抗原的应答如何,所有组分均有增强SK—SD刺激淋巴细胞转化作用,组分Ⅲ和Ⅳ的混合物作用最强。组分Ⅲ和Ⅳ对人的淋巴细胞亦有类似的作用。因此,恒河猴TF增强抗原刺激淋巴细胞转化的作用是没有抗原特异性和种属特异性的。 初步的生化分析表明,组分Ⅳ含有RNA,多肽和次黄嘌呤,薄层色谱有2个254nm紫外光吸收成分和2个节三酮显色成分,等电点聚焦电泳时出现17条考马斯兰R250着色带。 这些结果说明,有可能用恒河猴制备某些人类疾病的特异性TF。     

水稻无花药型不育材料的发现及其转育后代育性初步观察

水稻无花药型不育材料的发现及其转育后代育性初步观察①１宋德明１王志２刘永胜１王茂理１龙太康２孙敬三１（四川省绵阳市农业科学研究所绵阳６２１００２）２（中国科学院植物研究所北京１０００９３）关键词水稻，野生稻，无花药细胞质雄性不育ＤＥＶＥＬＯＰＭＥＮＴ...     

中国蟹蛛科新种记述(蛛形纲:蜘蛛目)

蟹蛛科(Thomisidae)是蜘蛛目中一个大科,常见于农田和园林,与人类关系密切。蟹蛛行动缓慢,常隐蔽于树叶、花草丛中捕食害虫。色泽艳丽,形态多样,并有拟态现象。 中国的蟹蛛研究报道尚不多,笔者近年来通过镜检大量标本,初步鉴定23属70种。本文限于篇幅,将新种先作一报道。我国幅员广阔,蟹蛛种数决不止此数,尚待今后进一步研究。     

流行性出血热病毒R22株cDNA克隆及其特异性鉴定

用家鼠型流行性出血热病毒R22株RNA,经polyA接尾,以Oligo-dT做引物,合成cDNA。用pUC18为载体转染E.coli Mc1061,建立cDNA克隆。再经菌落杂交,选择病毒特异性的5个阳性克隆制成缺口翻译探针,与病毒RNA3个片段进行反杂交,确定RNA片段的特异性。结果表明,3个克隆为中(M)片段的cDNA,另两个分别为大(L)和小(S)片段cDNA。核苷酸序列分析证明,克隆的DNA中含病毒特异的核苷酸序列。     

尾壳核内注射脑啡肽和bestatin对大鼠操作式条件...

Female Wistar rats were trained in a Skinner-box, 30 trials per day in a dark room to establish operant defence conditioning. Training started with a light (15 s), then combined with footshock for further 8 s. When the rats learned to press the key to avoid footshock within 15 s, conditioned response was considered established. After the rats reached a conditioning rate (CR) above 80% for 5 days, cannulae were implanted into caudate-putamen. Two to three days later, Met-enkephalin (MEK) or bestatin (an aminopeptidase inhibitor) was injected bilaterally into caudate-putamen. 30 min, 2 h, 24 h and 48 h after injection, conditioning tests were conducted, with each session consisting of 30 trials. Control experiments were done when 0.9% NaCl (NS) was injected. After injection of NS, CR maintained above 80% in all 4 test sessions. MEK (60 ng/rat) or bestatin (10 micrograms/rat) significantly lowered the CR during the 30 min and 2 h test session. In the latter case, the latency (L) was also prolonged. However both CR and L returned to the control level in the 24 h and 48 h test sessions. Naloxone (2 mg/kg, i.p.) blocked the conditioning-depression effect of bestatin. No significant alteration was seen in locomotor activity after MEK or bestatin injection. The results suggest that enkephalin in caudate-putamen may be involved in the regulation of retrieval of conditioning. Bestatin mimics the effect of MEK on conditioning reflex probably by increasing production of endogenous enkephalin.     

浙江猫蛛科一新种：蜘蛛目

在浙江三门发现猫蛛科一新种,暂归人哈猫蛛属,定名为三门哈猫蛛Hamataliwa sanmenensis sp.nov.本新种与三突哈猫蛛H.tricuspidata相近似,但有明显区别。本属为我国新纪录属。     

中国宁夏盖蛛属一新种：（蜘蛛目：皿蛛科）

本文记述蜘蛛目皿蛛科皿蛛亚科盖蛛属一新种——六盘盖蛛,新种Neriene liupanensis。标本采自宁夏六盘山自然保护区。     

大斑啄木鸟取食行为的研究

大斑啄木鸟(Dendrocopos major)是左家地区的常见鸟类,对它的研究,国内有过一些报道。1990年4—5月,笔者对它的取食行为做了较为系统的观察,现整理报道如下。