发育中小鼠和鸡胚脑微管蛋白的合成与甲状腺功能间的关系

利用 ³H-秋水仙碱与微管蛋白间的特异结合及DEAE纤维素对微管蛋白的离子交换作用,连续测定小鼠、鸡胚脑发育过程中的脑微管蛋白的合成变化。结果表明脑微管蛋白的合成速度均在其脑发育的临界期时达到最高峰。此时恰是甲状腺功能逐渐完善的时期。当小鼠进入育龄期时,雌雄鼠脑微管蛋白含量差异显著。可能说明性激素对微管蛋白的合成有重要影响。     

Effects of the Development of Thyroid and Sex Glands on the Synthesis of Brain Tubulin in Chick and Mouse Embryos

Using the specific affinity of tubulin for colchicine and the strong absorption of tubulin to DEAEion exchangers at neutral pH and moderate ionic strength,the amounts of tubulin in the brain fromboth mice and chicks during different developing stages were measured by ~3H-colchicine assay(expressed as colchicine binding activity).The results show that the rate oftubulin synthesis reacheda peak value during the critical period of brain development.This is exactly the period during whichthe organization and function of thyroid are being perfected.Besides,during breeding period,thedifference of tubulin contents between male and female is significant(P<0.001).The synthesis oftubulin is strictly sex dependent(this phenomenon appeared only during sex maturation stage).It issuggested that sexual hormones might exert their effect on tubulin synthesis.     

DEAE纤维素法测定微管蛋白

利用 ³H-秋水仙碱与微管蛋白的特异性结合及DEAE纤维素对微管蛋白的离子交换作用,以测定 ³H-秋水仙碱微管蛋白复合物的含量,了解脑神经原的分化状态。此法简便、快速,稳定、经济。