The interaction between ADAM22 and 14-3-3β

ADAM family consists of a number of transmembrane proteins that contain a disintegrin and metalloprotease domain. ADAMs are involved in a highly diverse set of biological processes, including fertilization, neurogenesis, myogenesis and inflammatory response. The ADAM proteins have both cell adhesion and protease activities.Adam22 is highly expressed in human brain. Theadam22-/- mice presented severe ataxia and died before weaning, but the function of ADAM22 is still unknown. 14-3-3 β interacting with ADAM22 was detected by using yeast two-hybrid assay. The specificity of interaction between ADAM22 and 14-3-3β was proved byin vitro binding assay and immunoprecipitation. The major 14-3-3β binding site was located in the last 28 amino acid residues of ADAM22 cytoplasmic tail. Protein 14-3-3β is abundant and plays an important role in mediating cell diffusion, migration and cell cycle control. The interaction of ADAM22 and 14-3-3β suggests that the ADAM22 may play a crucial role in neural function and development.     

Preliminarily functional analysis of a cloned novel human

ADAM is a family of type I integral membrane proteins which are characterized by sharing a disintegrin and metalloprotease domain and involved in many important physiological processes such as fertilization, neurogenesis and inflammatory response. A novel human ADAM gene--ADAM29, which was cloned in our laboratory, is exclusively expressed in human testis andcontains a potential fusion domain. A full-length cDNA of ADAM29 was obtained by using multiple-step PCR. Phylogenetic tree of known mammalian ADAMs specifically expressed in testis was reconstructed. Polyclonal antiserum was raised by immunizing the rabbits with sub-peptide of ADAM29 (Leu268-Asp374) as immunogen. The result of immunohistochemical test on human testis showed that ADAM29 is expressed in different stages of spermatogenesis and in interstitial cells. ADAM29 may play a certain role in the signal transduction during the maturation of tes-tis-associated cells.     

基于野外调查和同质种植园实验的芦苇植物功能性状变异研究

研究表型可塑性和遗传变异在植物表型分化中的相对作用,有助于预测全球环境变化下的植物群落组成和生态系统功能的变化。芦苇（Phragmites australis）是全球性广布的草本植物,种内变异丰富,在我国西北和东部均存在多个分化稳定的生态型,但中国芦苇在更大尺度上的表型研究还非常匮乏。将位于黄河上游的宁夏平原和黄河下游的黄河三角洲作为研究区域,通过野外调查和同质种植园实验对芦苇自然种群的植物功能性状变异进行观测。结果表明,无论在野外还是同质种植园,黄河三角洲芦苇的基径、叶长和叶宽均显著大于宁夏平原芦苇,说明两个地区的芦苇种群之间存在着受遗传决定的表型分化,这可能与两个地区间的降水等气候差异有关。在野外,宁夏芦苇的株高和叶厚显著大于黄河三角洲芦苇,但在同质园中差异消失或相反,说明株高、叶厚受环境影响较大,表型可塑性也是芦苇适应环境变化的重要机制。在同质种植园中,宁夏平原芦苇的叶片氮磷含量较低,但株数却显著多于黄河三角洲芦苇,反映了不同地区芦苇之间存在不同的适应策略,宁夏平原芦苇更偏向于高扩散率的杂草策略,而黄河三角洲芦苇更偏向于竞争策略。此外,宁夏平原芦苇的株高、叶长两个性状以及基径-比叶面积相关性在野外和同质园两个环境中存在一致性,表明了性状变异和权衡策略的遗传稳定性。综上,位于黄河上下游的芦苇种群间存在着适应性分化,这是表型可塑性和遗传变异共同作用的结果,不同来源芦苇对全球变化下的多重环境因子的响应还需要进一步研究。     

博落回SSR引物的开发以及遗传多样性分析

根据前期由本实验室完成的博落回转录组数据设计出65对引物,以浏阳产地的博落回为材料进行筛选,得到了28对有效引物.用这些引物对来自全国4个不同主要产区的博落回进行扩增,共扩增出引物159条带,其中多态性条带121条,占76.10%,共找到特征性条带13条.统计并比较4个不同产地的数据,经研究发现不同地区的博落回的遗传多样性较低,4个不同产地的博落回相似性较高.     

ADAM家族的结构特征与生物学功能

ADAM家族(A Disintegrin And Metalloproteinase domain)是一类包括去整合域和金属蛋白酶域的跨膜蛋白分子,具有发育、细胞间相互作用、精卵结合、肌管融合、神经发育和肿瘤发生等功能。本文对ADAM家族的结构和主要功能进行介绍。     

抽动障碍患儿实验室检查及其诊断意义的分析

目的:统计抽动障碍住院患儿相关实验室检查,分析其对于抽动障碍诊断与鉴别诊断的意义。方法:抽取2013年1月至2014年9月在上海交通大学医学院附属新华医院中医科住院治疗的98例抽动障碍患儿各项主要实验室检查结果进行统计分析。结果:抗"O"异常率为17.35%,与对照组比较P0.05,铜蓝蛋白均值为0.2719±0.0477,与对照组比较P0.05,血沉异常率为4.08%,与对照组比较,P0.05。炎症因子均存在一定异常,异常率最高为IL-2r达14.29%,免疫球蛋白只有Ig M存在1例(1.02%),T细胞亚群各项均存在异常,其中CD4/CD8异常率达25.51%,CD3异常率为12.24%。肝肾功能、血尿常规各项异常率统计基本正常,仅仅少数存在少量异常;结论:抗"O"、血沉和铜蓝蛋白等对TD的诊断和鉴别诊断具有重要意义,同时也应该关注患儿的免疫情况。肝肾功能、血尿常规等项目的监测对选择用药具有指导意义。     

The interaction between ADAM22 and 14-3-3β

ADAM family consists of a number of transmembrane proteins that contain a disintegrin and metalloprotease domain. ADAMs are involved in a highly diverse set of biological processes, including fertilization, neurogenesis, myogenesis and inflammatory response. The ADAM proteins have both cell adhesion and protease activities. Adam22 is highly expressed in human brain. The adam22-/- mice presented severe ataxia and died before weaning, but the function of ADAM22 is still unknown. 14-3-3 β interacting with ADAM22 was detected by using yeast two-hybrid assay. The specificity of interaction between ADAM22 and 14-3-3β was proved by in vitro binding assay and immunoprecipitation. The major 14-3-3β binding site was located in the last 28 amino acid residues of ADAM22 cytoplasmic tail. Protein 14-3-3β is abundant and plays an important role in mediating cell diffusion, migration and cell cycle control. The interaction of ADAM22 and 14-3-3β suggests that the ADAM22 may play a crucial role in neural function a     

Preliminarily functional analysis of a cloned novel human gene ADAM29

ADAM is a family of type I integral membrane proteins which are characterized by sharing a disintegrin and metalloprotease domain and involved in many important physiological processes such as fertilization, neurogenesis and inflammatory response. A novel human ADAM gene--ADAM29, which was cloned in our laboratory, is exclusively expressed in human testis and contains a potential fusion domain. A full-length cDNA of ADAM29 was obtained by using multiple-step PCR. Phylogenetic tree of known mammalian ADAMs specifically expressed in testis was reconstructed. Polyclonal antiserum was raised by immunizing the rabbits with sub-peptide of ADAM29 (Leu268-Asp374) as immunogen. The result of immunohistochemical test on human testis showed that ADAM29 is expressed in different stages of spermatogenesis and in interstitial cells. ADAM29 may play a certain role in the signal transduction during the maturation of testis-associated cells.     

人新基因ADAM29功能的初步分析

ADAM家族是一类具有去整合素域和金属蛋白酶域的Ⅰ型跨膜蛋白,广泛参与各种重要的生理过程,如精卵结合、神经系统发育、成肌细胞融合以及炎症反应等.ADAM29是一个新发现的人类基因,它特异性地表达于人睾丸组织中,包含一段潜在的融合蛋白域.用PCR方法克隆了其全长cDNA,并比较分析了在哺乳动物睾丸组织中专一表达的ADAM家族成员的氨基酸顺序.此外,以ADAM29的一个肽段(Len268~Asp374)制成融合蛋白后作为抗原制备多克隆抗体.人睾丸组织切片免疫组织化学的检测结果表明,ADAM29分布于多种细胞内,但主要集中于间质细胞,因而推测ADAM29可能与信号传导有关.     

ADAM22蛋白与14-3-3β蛋白之间的相互作用

ADAM家族是一类具有去整合域和金属蛋白酶域的跨膜蛋白, 广泛参与各种重要的生理过程, 如精卵结合、神经系统发育、成肌细胞融合以及炎症反应等. 该家族蛋白具备潜在的粘连和蛋白酶活性. adam22在脑部高度表达, 基因剔除小鼠则出现严重的共济失调, 并在断奶前死亡, 但其作用机制不详. 用酵母双杂合系统筛选到与ADAM22相互作用的蛋白质14-3-3b, 离体结合实验、免疫共沉淀进一步证实了ADAM22与14-3-3β蛋白相互作用的专一性. ADAM22胞内部分系列缺失实验表明, C端第864~892氨基酸残基是14-3-3β的结合基序. 14-3-3β在脑部大量表达, 具有介导细胞的扩散、迁移及调节细胞周期等重要功能. 因此, 本实验证实这两种蛋白质之间存在相互作用,为阐明ADAM22在神经系统发育中的功能奠定了基础.