家蚕核型多角体病毒山东株p35基因的克隆及序列分析

根据家蚕核型多角体病毒T3株p35基因非编码区序列设计一对特异性引物,应用PCR技术从家蚕核型多角体病毒山东株中扩增得到一条1 080 bp的片段。测序结果表明,该片段含有p35基因,开放阅读框长900 bp,编码299个氨基酸,分子量为34.91kDa,pI=6.4。序列分析表明,BmNPV山东株的p35基因与T3株存在差异。 Abstract:According to the 5′ and 3′ untranslated region sequences of p35 gene from Bombyx mori nuclear polyhedrosis virus isolate T3,a pair of primers was designed employing computer analysis.With the primers,polymerase chain reaction (PCR) was performed and a 1 080 bp fragment was obtained from BmNPV strain Shandong.Sequencing result showed that it was p35 gene (GenBank accession number：AY157746),and it included an open reading frame of 900 bp,encoding 299 amino acids with Mr=34.91kDa and pI=6.40.BLAST analysis indicated that there were seven bases and three amino acids difference between p35 from BmNPV strain Shandong and that from BmNPV isolate T3.     

微孢子虫对马尾松毛虫生存率和繁殖力的影响

为了解微孢子虫对马尾松毛虫的影响,探索微孢子虫防治松毛虫的可能性,我们用家蚕微孢子虫Nosema bombycis Naegli.接种松毛虫进行试验,现将初步结果报道如下。 一、一龄幼虫接种试验 用孢子液浸卵和喷叶喂一龄虫都能使幼虫感病。微孢子虫对一龄松毛虫的染病情况和对生存率的影响见表1。