48例原发性闭经患者的细胞遗传学分析

本文报告对48例原发闭经患者的临床和细胞遣传学分析,共发现染色体异常17例,占35.4%,其中包括45,X,7例;45,X/46,XX,2例;X染色体结构异常5例;核型中有Y染色体3例。讨论了原发闭经的细胞遗传学病因及异常核型与表型的关系。     

罕见的46，X，inv(Xq)伴原发闭经一例

X染色体数目及结构异常是原发性闭经的重要原因。在各类X染色体结构异常中,未见有关X染色体长臂的臂内倒位方面的报道。我室在1555例(男832例,女723例)染色体检查的患者中发现了一例,现予报告。     

Sinoinsula,a name to replace Insula （Araneae,Salticidae）

Sinoinsula nom. nov. = Insuht Zhou ＆ Li, 2013. Zootaxa 3712 （I）： 11.     

Newly discovered insect RNA viruses in China

Insects are a group of arthropods and the largest group of animals on Earth,with over one million species described to date.Like other life forms,insects suffer from viruses that cause disease and death.Viruses that are pathogenic to beneficial insects cause dramatic economic losses on agriculture.In contrast,viruses that are pathogenic to insect pests can be exploited as attractive biological control agents.All of these factors have led to an explosion in the amount of research into insect viruses in recent years,generating impressive quantities of information on the molecular and cellular biology of these viruses.Due to the wide variety of insect viruses,a better understanding of these viruses will expand our overall knowledge of their virology.Here,we review studies of several newly discovered RNA insect viruses in China.     

产自东亚钳蝎的钠通道调节剂的抗癫痫/促癫痫作用（英文）

东亚钳蝎(Buthus martensii Karsch, BmK)是治疗癫痫的经典中药之一,已有上千年的历史。从BmK毒液中分离纯化的神经毒素被认为是作用于膜离子通道的主要活性成分。电压门控钠通道(voltage-gated sodium channels, VGSCs)在癫痫发生中起着重要的作用,使其成为重要的癫痫药物靶点。BmK的长链毒素由60～70个氨基酸残基组成,能特异性识别VGSCs,其中用于建立癫痫模型的α-样神经毒素与VGSC受体位点3结合,可诱发鼠类癫痫。而β或β-样神经毒素则与VGSC受体位点4结合,对癫痫模型有显著的抗惊厥作用。本综述旨在阐明BmK多肽作用于VGSCs的抗惊厥或惊厥作用,同时也为抗癫痫药物设计提供潜在的框架。     

PCR-RFLP检测LDL受体基因TaqⅠ多态性位点的研究

应用聚合酶链反应(PCR)扩增人类LDL受体基因外显子4-内含子4-外显子5片段,PCR产物为1.55kb,DNA片段经序列鉴定后,进行TaqI酶切位点的RFLP分析。结果显示:中国汉族人群LDL受体基因中存在着TaqⅠ酶切位点多态性; 200个LDL受体等位基因中TaqⅠ酶切位点出现的频率为0.515,该点频率较为适中, 可作为中国汉族人群LDL受体基 因的遗传标志来进行家族性高胆固醇血症(FH)的基因诊断。所建立起的LDL受体基因TaqⅠ位点的PCR -RFLP方法具有快速、简便的特点,在FH的基因诊断上有应用价值。 Abstract:To develop rapid and sensitive technique for detectin the TaqI polymorphism at the human LDL receptor gene in Chinese,the exon4-intron4-exon5 of the human LDL receptor gene was amplified by polymerase chain reaction(PCR).The PCR products were directly analysed by restriction fragment length polymorphisms(RFLP).The results showed that the TaqI polymorphism is associated with the LDL receptor gene in Chinese of Han nationality;The frequency of T= allele (presence of TaqI cutting site)is 0.515 in 200 LDL receptor alleles.This technique may be used for rapid and sensitive screening of the LDL receptor gene for the TaqI polymorphism.     

一种简单、经济的植物RNA抽提方法

一种简单、经济的植物ＲＮＡ抽提方法孟玲谭德勇王焕效吕朝晖王晓燕周翔（云南大学生物系,昆明６５００９１）ＡＳｉｍｐｌｅＥｃｏｎｏｍｉｃａｌＭｅｔｈｏｄｆｏｒｔｈｅＩｓｏｌａｔｉｏｎｏｆＰｌａｎｔＲＮＡＭＥＮＧＬｉｎｇＴＡＮＤｅｙｏｎｇＷＡＮＧＨｕａｎｘ...     

Detection and Genetic Characterization of Rabies Virus from Human Patients

Saliva and blood were collected from two patients who had not received post exposure prophylaxis in the cities of Wenzhou and Xinning respectively. Both patients were confirmed as positive for rabies by detection of rabies virus specific nucleoprotein antibodies in the sera by Western Blot. However, rabies virus specific RNA was only identified in the saliva collected from the patient in Wenzhou. Furthermore, the isolate Zhejiang Wz0 (H) was obtained by inoculating one-day-old suckling mice. Both nucleoprotein (N) and glycoprotein (G) genes from the isolate were amplified by RT-PCR and sequenced. Phylogenetic analysis indicated that the isolate belonged to classic rabies virus, and shared a higher homology with the street viruses from dogs in the main endemic areas in China and the street virus from dogs in Indonesia than with other known strains. Further comparison of the deduced amino acid sequences between the isolate and the vaccine strains used in China showed that the virus had a higher level of homology with the vaccine strain CTN than with the other vaccine strains (3aG, PV, PM and ERA). In particular, amino acid residues substitutions located in antigenic site Ⅲ in the G protein, which could react with the neutralizing antibodies, were observed. These results suggested that the virus belonged to the classic rabies virus, and both N and G genes diverged from the current vaccine strains used in China at either the nucleotide or the amino acid level.