DNA的免疫激活作用--Ⅱ.应用基础研究进展

综述免疫激活序列(ISS)应用基础方面的研究进展.ISS-DNA不仅有望成为新一代高效低毒的免疫佐剂,而且在抗肿瘤、抗感染、促进造血、治疗免疫缺陷以及防治哮喘等方面均有非常乐观的应用前景.     

The influence of Annexin32, a new Ca2+-dependent phospholipid-binding protein, on coagulation time and thrombosis

The pharmacodynamics of Annexin32, a new Ca2+-dependent phospholipid-binding protein, was studied by measuring coagulation time in rabbits and venous thrombosis in rabbits and rats. Rabbits and rats were given Annexin32 by intravenous administration. Then Kaolin partial thromboplastin time (KPTT), thrombosis in vitro and in vivo were assayed. The results showed that KPTT of rabbits was prolonged (p < 0.01), and the length and weight of thrombus in vitro were reduced (p < 0.01) after administration of Annexin32 at 1 mg/kg. It also inhibited thrombosis in vivo and reduced the weight of venous thrombus significantly in rats (p < 0.01). All these results suggested that Annexin32 possesses the characteristic of antithrombotic effect and fewer side effects on coagulation time.     

强直性脊柱炎易感基因的研究进展

强直性脊柱炎（ankylosing spondylitis,AS）是一种常见的高度遗传的风湿类疾病。至20世纪70年代以来,越来越多的证据表明HLA-B27是AS最主要的易感基因。然而,全基因组扫描和关联分析等研究发现在HLA以外的区域仍存在AS的易感区域,大量的证据显示在HLA区内外有许多基因与AS的发病有关。文章主要综述了与AS相关的易感基因以及它们的研究现状。     

绿色荧光蛋白作报告基因的研究

海洋无脊椎动物的生物发光现象源于一类绿色荧光蛋白(green fluorescent proteins,GFPs)。自1992年Aequorea GFP cDNA被克隆后,GFP作为一个新的报告基因已取得了广泛的应用。 GFP-S65T是野生型GFP的Ser65→Thr的一个突变体,它的密码子按真核生物的密码     

DNA的免疫激活作用——Ⅰ.作用机理研究进展

含有免疫激活序列的DNA或寡聚核苷酸对脊椎动物的免疫系统可以产生广泛的激活作用。本文综述DNA免疫激活作用的结构基础、细胞及分子作用机制等方面的研究进展。     

DNA疫苗的细菌内毒素检测

目的 :考察DNA疫苗细菌内毒素的检查方法的可行性及DNA疫苗的干扰作用。方法 :干扰试验和对比试验。结果 :供试品阴性对照系列样品溶液无干扰作用 ,与家兔法结果一致。结论 :将疫苗稀释 2倍可用灵敏度为 0 5EU ml的鲎试剂作细菌内毒素检查。     

囊尾蚴膜联蛋白32在大肠杆菌中的表达及纯化

在已获得的囊尾蚴膜联蛋白 (Annexin) 32cDNA的基础上 ,以PCR的方法在cDNA两端加上酶切位点 ,插入原核表达载体pJLA 5 0 3,热诱导表达后 ,外源蛋白大部分以可溶形式表达 ,表达量占菌体总蛋白的 35 %。经(NH₄)₂SO₄分级沉淀、DEAE阴离子交换层析及Sephacry1S 2 0 0凝胶过滤层析得到电泳单一条带 ,Westernblot及抗凝血实验证明表达产物是有生物活性的     

The influence of Annexin32, a new Ca2+-dependent phospholipid-binding protein, on coagulation time and thrombosis

The pharmacodynamics of Annexin32, a new Ca2+-dependent phospholipid-binding protein, was studied by measuring coagulation time in rabbits and venous thrombosis in rabbits and rats. Rabbits and rats were given Annexin32 by intravenous administration. Then Kaolin partial thromboplastin time (KPTT), thrombosis in vitro and in vivo were assayed. The results showed that KPTT of rabbits was prolonged (p < 0.01), and the length and weight of thrombus in vitro were reduced (p < 0.01) after administration of Annexin32 at 1 mg/kg. It also inhibited thrombosis in vivo and reduced the weight of venous thrombus significantly in rats (p < 0.01). All these results suggested that Annexin32 possesses the characteristic of antithrombotic effect and fewer side effects on coagulation time.     

DNA的免疫激活作用 Ⅰ．作用机理研究进展

含有免疫激活序列的DNA或寡聚核苷酸对脊核动物的免疫系统可以产生广泛的激活作用。本文综述DNA免疫激活作用的结构基础、细胞及分子作用机制等方面的研究进展。     

小鼠CCR5基因在活体内表达受迟发型超敏反应的调节

趋化因子(chemokine)在机体的免疫监视和慢性炎症反应中起着重要作用。β-趋化因子受体5(CCR5)是巨噬细胞嗜性(M-tropic)HIV-1进入靶细胞的一个重要辅助因子。作用在成功克隆一新的趋化因子受体5(muCCR5)中研究了该基因在小鼠活体内的表达。结果表明,muCCR5 cDNA全长2888 bp,ORF为