飞行人员疗养中的心理护理探讨

随着现代医学模式的转变和疗养康复事业的发展,疗养护理工作的内涵也在不断地拓宽和充实。我院常年担任着飞行人员的疗养任务,他们在疗养期间除了进行全面体检、疾病矫治、航空生理和体能训练、健康教育外,还应根据飞行人员的职业特点,做好飞行人员的心理护理,使飞行人员在疗养     

Analysis of Vero cell growth behavior on microcarrier by means of environmental scanning electron microscopy

By using environmental scanning electron microscopy, the morphological changes of Vero cells attached to and grown on the microcarrier Cytodex-3 were observed, and their behavior of adhesion, spreading and proliferation was analyzed. The effect of exogenous fibronectin/ laminin on adhesion and spreading of MCC/Vero cell was studied. The images of ESEM showed that expansion of cell growth was directed toward vacancy space. The growth curve and cell concentration change during the whole culture process were obtained from the statistical counting method based on ESEM images and the crystal violet method. The growth rate of Vero cells increases with increasing the concentration of cell inoculation, that is, the specific growth rate increases quickly with increasing the concentration of cell inoculation. When serum concentration in medium #199 ranged from 5% to 10%, experimental results indicated that serum concentration is one of the important factors influencing cell growth, particularly in the cell adhesio     

浅谈如何提供多元化文化环境下的护理

随着社会经济的发展,科学文化的广泛交流,跨世纪的护理人才必须掌握多元化文化护理,才能理解不同文化背景患者的需要,按照不同特点提供高水平、全方位的护理。但是在目前的临床实践中,往往偏重满足患者的生理需要,而忽略了心理、社会的需要,因此,护理工作中的文化安全性问题应引起重视。     

用环境扫描电子显微镜研究Vero细胞在微载体上的生长

使用环境扫描电子显微镜(ESEM), 通过观察Vero细胞在Cytodex-3微载体表面上贴附铺展及增殖生长过程中的形态变化, 分析了细胞在微载体上的贴附与铺展的过程和行为; 考察了外源的层/纤粘连蛋白对细胞铺展形貌的影响, 发现细胞在微载体上的增殖生长以细胞群落延伸扩展的形式进行. 用ESEM图像计数法和结晶紫细胞核计数法获得细胞生长过程的细胞密度变化和生长曲线, 研究了接种密度对细胞生长过程的影响, 随着接种密度的增大, 细胞延迟期缩短, 表观生长速率增大, 细胞长满微载体表面所用的时间缩短. 在实验的血清浓度范围内(5%~10%), 血清浓度影响细胞的贴附, 随着血清浓度的增大, 细胞的贴附率增加, 从而影响整个生长过程.     

Analysis of Vero cell growth behavior on microcarrier by means of environmental scanning electron microscopy

By using environmental scanning electron microscopy, the morphological changes of Vero cells attached to and grown on the microcarrier Cytodex-3 were observed, and their behavior of adhesion, spreading and proliferation was analyzed. The effect of exogenous fibronectin/ laminin on adhesion and spreading of MCC/Vero cell was studied. The images of ESEM showed that expansion of cell growth was directed toward vacancy space. The growth curve and cell concentration change during the whole culture process were obtained from the statistical counting method based on ESEM images and the crystal violet method. The growth rate of Vero cells increases with increasing the concentration of cell inoculation, that is, the specific growth rate increases quickly with increasing the concentration of cell inoculation. When serum concentration in medium #199 ranged from 5% to 10%, experimental results indicated that serum concentration is one of the important factors influencing cell growth, particularly in the cell adhesion and spreading stage.