全文获取类型
收费全文 | 486篇 |
免费 | 25篇 |
国内免费 | 199篇 |
出版年
2024年 | 4篇 |
2023年 | 9篇 |
2022年 | 11篇 |
2021年 | 8篇 |
2020年 | 5篇 |
2019年 | 5篇 |
2018年 | 9篇 |
2017年 | 5篇 |
2016年 | 12篇 |
2015年 | 15篇 |
2014年 | 23篇 |
2013年 | 22篇 |
2012年 | 75篇 |
2011年 | 90篇 |
2010年 | 17篇 |
2009年 | 17篇 |
2008年 | 60篇 |
2007年 | 44篇 |
2006年 | 33篇 |
2005年 | 63篇 |
2004年 | 29篇 |
2003年 | 22篇 |
2002年 | 13篇 |
2001年 | 24篇 |
2000年 | 7篇 |
1999年 | 19篇 |
1998年 | 9篇 |
1997年 | 8篇 |
1996年 | 9篇 |
1995年 | 9篇 |
1994年 | 4篇 |
1993年 | 11篇 |
1992年 | 1篇 |
1986年 | 2篇 |
1985年 | 1篇 |
1983年 | 1篇 |
1959年 | 1篇 |
1958年 | 4篇 |
1957年 | 1篇 |
1956年 | 2篇 |
1955年 | 1篇 |
1954年 | 2篇 |
1953年 | 1篇 |
1952年 | 2篇 |
排序方式: 共有710条查询结果,搜索用时 15 毫秒
1.
大豆灰斑病抗性遗传的三点测交分析 总被引:1,自引:0,他引:1
本实验利用三点测交分析的方法, 对3个组合在人工接种大豆灰斑病菌的条件下的抗性表现进行基因效应分析,各组合均存在加性,组合1存在显性,组合2、3存在上位性。
Abstract:In this paper,Triple Test Cross Design was used in studing the resistance of soybean to 10 physiological race of Cercospora Sojina Haraby inoculation.Results o analysis of gene effects of resistance indicated that additive effect is significant in all the three crosses,dominant effect exsists only in the cross 1 and epistatic effect remains in the cross 2 and cross 3. 相似文献
2.
无血清培养基培养人全血制备染色体研究谭湘陵,刘梅江苏省南通医学院生物学教研室,226001通过人外周血的培养制备染色体是最常用的方法。常规培养基中含一定量的血清,血清的使用被认为必不可少,但也存在一定的弊端[1]。国外对于细胞无血清培养技术进行了广泛... 相似文献
3.
本文探讨了用一种特殊形式资料估计遗传力的方法,亦可作为为扩大样本含量,充分利用不同来源且形式不同资料合并估计遗传力的方法。 相似文献
4.
连锁值——表述基因连锁强度的新参数 总被引:1,自引:0,他引:1
在遗传学中,同一染色体上基因之间的连锁强度是用交换率表示的.一般而言,交换率越高,基因连锁强度越低.但交换率不是基因连锁强度的最方便的量度,因为利用交换率来计算具有连锁交换现象的杂交后代分离比例时,必须区分相引组和相斥组两种不同的亲本交配情形.在相引组,一个亲本具有两个显性性状,另一个亲本具有两个隐性性状;在相斥组,两个亲本各具有一个显性性状和一个隐性性状。这两种情形下的配子比例和F_2分 相似文献
5.
LI Mi LIU LianQing XI Ning WANG YueChao DONG ZaiLi XIAO XiuBin ZHANG WeiJing 《中国科学:生命科学英文版》2013,56(9):811-817
Atomic force microscopy (AFM) was used to examine the morphology of live mammalian adherent and suspended cells. Time-lapse AFM was used to record the locomotion dynamics of MCF-7 and Neuro-2a cells. When a MCF-7 cell retracted, many small sawtooth-like filopodia formed and reorganized, and the thickness of cellular lamellipodium increased as the retraction progressed. In elongated Neuro-2a cells, the cytoskeleton reorganized from an irregular to a parallel, linear morphology. Suspended mammalian cells were immobilized by method combining polydimethylsiloxane-fabricated wells with poly-L-lysine electrostatic adsorption. In this way, the morphology of a single live lymphoma cell was imaged by AFM. The experimental results can improve our understanding of cell locomotion and may lead to improved immobilization strategies. 相似文献
6.
YANG Lei RONG WeiQi XIAO Ting ZHANG Ying XU Bin LIU Yu WANG LiMing WU Fan QI Jun ZHAO XiuYing WANG HongXia HAN NaiJun GUO SuPing WU JianXiong GAO YanNing CHENG ShuJun 《中国科学:生命科学英文版》2013,56(7):638-646
For successful therapy, hepatocellular carcinoma (HCC) must be detected at an early stage. Herein, we used a proteomic approach to analyze the secretory/releasing proteome of HCC tissues to identify plasma biomarkers. Serum-free conditioned media (CM) were collected from primary cultures of cancerous tissues and surrounding noncancerous tissues. Proteomic analysis of the CM proteins permitted the identification of 1365 proteins. The enriched molecular functions and biological processes of the CM proteins, such as hydrolase activity and catabolic processes, were consistent with the liver being the most important metabolic organ. Moreover, 19% of the proteins were characterized as extracellular or membrane-bound. For validation, secretory proteins involved in transforming growth factor-β signaling pathways were validated in plasma samples. Alphafetoprotein (AFP), metalloproteinase (MMP)1, osteopontin (OPN), and pregnancy-specific beta-1-glycoprotein (PSG)9 were significantly increased in HCC patients. The overall performance of MMP1 and OPN in the diagnosis of HCC remained greater than that of AFP. In addition, this study represents the first report of MMP1 as a biomarker with a higher sensitivity and specificity than AFP. Thus, this study provides a valuable resource of the HCC secretome with the potential to investigate serological biomarkers. MMP1 and OPN could be used as novel biomarkers for the early detection of HCC and to improve the sensitivity of biomarkers compared with AFP. 相似文献
7.
8.
DUAN AiPing NING LiMin LI Chao HOU YaFei YANG NaNa SUN LiZhou LI GenXi 《中国科学:生命科学英文版》2013,56(4):293-297
Hepatitis C virus (HCV), a positive single-stranded RNA virus, is a major cause of liver disease in humans. Herein we report a novel strategy to inhibit the reproduction and translation of HCV using a short RNA, named an Additional RNA, to activate the endonuclease activity of Argonaute 2 (Ago2). In the presence of the Additional RNA, the HCV genome RNA has the requisite 12 nucleotides of base-pairing with microRNA-122. This activates the endonuclease activity of Ago2, resulting in cleavage and release of the HCV genome RNA from Ago2 and microRNA-122. The free HCV genome RNA would be susceptible to intracellular degradation, effectively inhibiting its reproduction and translation. This study presents a new method to inhibit HCV that may hold great potential for HCV treatment in the future. 相似文献
9.
从阿维菌素获得诺贝尔奖到中国创造 总被引:1,自引:0,他引:1
纵观世界历史,大国崛起无不伴随着科技的兴起和机制体制的突破。来自大自然的天然产物对于人类的健康起到非常重要的作用,从抗肿瘤明星分子紫杉醇到挽救了无数人生命的抗感染药物青霉素,从治疗代谢疾病到营养保健,都离不开天然产物。此外,还有大量天然产物资源没有被开发过。而随着阿维菌素的发现者Satoshiōmura教授和William C.Campbell博士,及青蒿素的发现者屠呦呦研究员因为这两种天然产物在治疗寄生虫感染病和疟疾上的应用而获得2015年诺贝尔生理与医学奖后,天然产物有望迎来其发展的第二个黄金时代。我国是世界工厂也是天然药物的资源大国,为了实现产业升级,弯道超车,实现大国崛起的中国梦,我国科学家在"十二五"期间围绕着天然产物的高产和创新两大主题开展了富有成效的合成生物学研究。阿维菌素是由阿维链霉菌产生的高效低毒生物杀虫剂,其原料产能占国际市场的100%。但我国原有生产菌株的单位发酵产量较低,高消耗、高污染、片面追求生产规模的粗放型发展模式已经成了阻碍低碳经济持续高速发展的瓶颈。如何从根本上解决问题,提高生产菌株的单位发酵产量和原料利用率,降低能耗和生产成本,减少环境污染,是促进我国从"发酵大国"向"发酵强国"转变的关键。本文以阿维菌素为例,综述其基础研究的技术发展,特别是中国科学院微生物研究所引入合成生物学技术,将阿维菌素的单位产量提高了1000倍,至9 g/L,在内蒙古新威远与阿维菌素产业联盟的公司应用,迫使默克公司全面退出阿维菌素历史舞台,从而引领产业迅速发展的过程,为我国其它天然产物生物制造品种的改良提供思路和方法。 相似文献
10.
Four seasons of investigations on macrofauna in Yueqing Bay, China were carried out from August 2002 to May 2003. One hundred and twenty four species of macrofauna were identified, including 41 species of polychaeta, 37 species of mollusc, 22 species of crustacea, 10 species of echinoderm and 14 others. The average biomass was 41.95 g·m−2 and the average density was 85 ind·m−2. The echinoderm contributed the highest proportion (about 60%) to the total biomass, and the mollusc contributed the maximum proportion (about 35%) to the total density. Biomass and density in spring were higher than those in other seasons. This article analyzes the macrofauna distribution, compares the study results with the history data of Yueqing Bay and adjacent bays, and discusses the response of macrofauna community to the aquiculture in Yueqing Bay. 相似文献