全文获取类型
收费全文 | 3775篇 |
免费 | 194篇 |
国内免费 | 155篇 |
出版年
2024年 | 15篇 |
2023年 | 38篇 |
2022年 | 2篇 |
2021年 | 20篇 |
2020年 | 4篇 |
2019年 | 28篇 |
2018年 | 16篇 |
2017年 | 12篇 |
2016年 | 15篇 |
2015年 | 12篇 |
2014年 | 28篇 |
2013年 | 17篇 |
2012年 | 707篇 |
2011年 | 683篇 |
2010年 | 99篇 |
2009年 | 74篇 |
2008年 | 391篇 |
2007年 | 392篇 |
2006年 | 329篇 |
2005年 | 297篇 |
2004年 | 254篇 |
2003年 | 203篇 |
2002年 | 155篇 |
2001年 | 108篇 |
2000年 | 111篇 |
1999年 | 56篇 |
1998年 | 11篇 |
1997年 | 5篇 |
1996年 | 6篇 |
1995年 | 6篇 |
1994年 | 4篇 |
1993年 | 5篇 |
1992年 | 5篇 |
1991年 | 6篇 |
1990年 | 2篇 |
1989年 | 2篇 |
1988年 | 1篇 |
1987年 | 1篇 |
1986年 | 1篇 |
1985年 | 2篇 |
1981年 | 1篇 |
排序方式: 共有4124条查询结果,搜索用时 41 毫秒
1.
Fang Chang An Yan Li-Na Zhao Wei-Hua Wu Zhenbiao Yang 《植物学报(英文版)》2007,49(8):1261-1270
A tip-focused Ca^2+ gradient is tightly coupled to polarized pollen tube growth, and tip-localized influxes of extracellular Ca^2+ are required for this process. However the molecular identity and regulation of the potential Ca^2+ channels remains elusive. The present study has implicated CNGC18 (cyclic nucleotide-gated channel 18) in polarized pollen tube growth, because its overexpression induced wider and shorter pollen tubes. Moreover, CNGC18 overexpression induced depolarization of pollen tube growth was suppressed by lower extracellular calcium ([Ca^2+]ex). CNGC18-yellow fluorescence protein (YFP) was preferentially localized to the apparent post-Golgi vesicles and the plasma membrane (PM) in the apex of pollen tubes. The PM localization was affected by tip-localized ROP1 signaling. Expression of wild type ROP1 or an active form of ROP1 enhanced CNGC18-YFP localization to the apical region of the PM, whereas expression of RopGAP1 (a ROP1 deactivator) blocked the PM localization. These results support a role for PM-Iocalized CNGC18 in the regulation of polarized pollen tube growth through its potential function in the modulation of calcium influxes. 相似文献
2.
3.
<正> 静注免疫球蛋白制剂用于替代疗法,特别适于在短时期内需输注大量的免疫球蛋白时。例如对早产儿和严重感染的足月新生儿、原发性或继发性低或无丙种球蛋白血症患者,以及骨髓移植一个月后的病人,都是需要的。在该种情况下,免疫球蛋白的肌肉注射几乎是不可能的,因为,需要量大,有时在几天内,会使病人相当的痛苦和不愉快。 相似文献
4.
SRY盒基因在斑马鱼和胡子鲇中的保守性分析 总被引:9,自引:2,他引:7
本文采用人类SRY基因探针,与EcoRI消化的中国的胡子鲇(Clarias fusc us)和美国的斑马鱼(Brachydanio rerio)基因组DNA进行了Southern印迹杂交分析。首次在这两种鱼中发现了SRY盒基因及其存在特征;在斑马鱼中发现了明显的限制性片段长度多态性; 并深入分析了SRY盒基因的进化保守性。
Abstract:Using,Southern bloting analysis,EcoRI-digested genomic DNA from Clarias fuscus and Brachydanio rerio were hybridizae to human SRY probe.Hybridization occurred in both male and female of the two species.RFLP associated with SRY-box genes was observed in Brachydanio rerio.The conservation of SRY-box genes was analysed. 相似文献
5.
用地高辛标记原位杂交技术定位黄鳝rRNA基因于二价染色体3q12—q24和7… 总被引:9,自引:2,他引:7
以地高辛标记重组质粒PTA71中所含的小麦rRNA基因作为探针,与EcoRI酶切的黄鳝核基因组点DNA经Southern杂交,呈现2条带,片段长度分别为12.8kb和4.6kb。再运用染色体原位杂交技术及杂交一多重带显带技术,定位rRNA基因于黄鳝二价染色体3q12-q24和7q14-q26两个区间,其分布位点与硝酸银染法结果相符。此外,还讨论了在黄鳝二价体上开展基因定位研究的突出优点。 相似文献
6.
Wang Banyue 《古脊椎动物学报》1994,(4)
Inl988-199otheRegionalGeologicalSurveyTeamoftheGeologicalBureauofNingxia(RGSN)foundsomefossilmammalsinthelowerpartoftheHo11g1iugouFormationandtheupperpartoftheOligocene.Inl992ajointteamoflnstituteofVertebratePaleontologyandPaleoanthropo1ogy,AcademiaSinica(IVPP),andtheRGSNwenttoHaiyuailCountyandcollectedmorefossilmamma1sfromtheYuanjia-wowoareausi11gascreen-washingmethodThemammalfaunafromthelowerpartoftheHongliugouFormationiscomposedofAprotodonsp.,Indricotheriidaeindet.,Rhinoceroti… 相似文献
7.
Xuexue Chen Yanglin Ding Yongqing Yang Chunpeng Song Baoshan Wang Shuhua Yang Yan Guo Zhizhong Gong 《植物学报(英文版)》2021,63(1):53-78
Protein kinases are major players in various signal transduction pathways. Understanding the molecular mechanisms behind plant responses to biotic and abiotic stresses has become critical for developing and breeding climate-resilient crops. In this review,we summarize recent progress on understanding plant drought, salt, and cold stress responses, with a focus on signal perception and transduction by different protein kinases, especially sucrose nonfermenting1(SNF1)-related protein kinases(Sn RKs),mitogen-activated protein kinase(MAPK) cascades,calcium-dependent protein kinases(CDPKs/CPKs),and receptor-like kinases(RLKs). We also discuss future challenges in these research fields. 相似文献
8.
A redox-active [Ru(bpy)(2)(tatp)](2+)-BSA-SWCNTs (bpy=2,2'-bipyridine, tatp=1,4,8,9-tetra-aza-triphenylene, BSA=bovine serum albumin, SWCNTs=single-walled carbon nanotubes) hybrid film is fabricated on an indium-tin oxide (ITO) electrode via one-step electrochemical co-assembly approach. BSA is inherently dispersive and therefore served as the linking mediator of SWCNTs, which facilitate the redox reactions of [Ru(bpy)(2)(tatp)](2+) employed as a reporter of BSA. The evidences from differential pulse voltammetry, cyclic voltammetry, scanning electron microscope, emission spectroscopy and fluorescence microscope reveal that the [Ru(bpy)(2)(tatp)](2+)-BSA-SWCNTs hybrid can be electrochemically co-assembled on the ITO electrode, showing two pairs of well-defined Ru(II)-based redox waves. Furthermore, the electrochemical co-assembly of the [Ru(bpy)(2)(tatp)](2+)-BSA-SWCNTs hybrid is found to be strongly dependent on the simultaneous presence of BSA and SWCNTs, indicating a good linear response to BSA in the range from 6 to 50mgL(-1). The results from this study provide an electrochemical co-assembly method for the development of non-redox protein biosensors. 相似文献
9.
The Genetic and Molecular Basis of Plant Resistance to Pathogens 总被引:1,自引:0,他引:1
Plant pathogens have evolved numerous strategies to obtain nutritive materials from their host,and plants in turn have evolved the preformed physical and chemical barriers as well as sophisticated two-tiered immune system to combat pathogen attacks.Genetically, plant resistance to pathogens can be divided into qualitative and quantitative disease resistance,conditioned by major gene(s) and multiple genes with minor effects,respectively.Qualitative disease resistance has been mostly detected in plant defense against biotrophic pathogens,whereas quantitative disease resistance is involved in defense response to all plant pathogens,from biotrophs,hemibiotrophs to necrotrophs.Plant resistance is achieved through interception of pathogen-derived effectors and elicitation of defense response.In recent years,great progress has been made related to the molecular basis underlying host-pathogen interactions.In this review,we would like to provide an update on genetic and molecular aspects of plant resistance to pathogens. 相似文献
10.
Liangliang Yu Leqi Huang Shuang Zeng Guirong Tang Sunjun Wang Ningning Li Junhui Yan Li Luo 《Acta biochimica et biophysica Sinica》2019,(4):448-451
ROS include superoxide anion radical (O2^-), hydrogen peroxide (H2O2), singlet oxygen (0-0), hydroxyl radical (OH ), and hydroxyl anion (OH-). O2^-, the radical with the highest oxidation compacity to produce H2O2, OH and OH^-, is a byproduct of aerobic respiration in organisms. Rhizobia and their legume hosts are aerobic organisms, and nitrogen-fixing nodules formed on legume roots are developed from their symbiotic in teractions. Therefore, ROS are generated in legume nodules through aerobic respiration by both symbiotic partners. 相似文献