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Summary Effects of osmotic conditions on secretion of milk serum were examined using standard transmission electron microscopy. Rat mammary glands were infused with hyper-, iso-, and hypo-osmotic solutions. The intramammary infusion of these agents elicited distinct and repeatable morphological responses from lactating epithelial cells. The response to hyperosmolarity was an increase in compound exocytotic figures and an increase in secretory vesicle size (¯x=1.65 m in diameter). Glands infused with hypo-osmolar solutions exhibited the opposite response; reduction in compound exocytotic figures and reduced vesicle size (¯x=0.34 n in diameter). The response to iso-osmotic solutions was indistinguishable from untreated control tissue. The ratio of vesicular projections to depressions (vesicle membrane/plasma membrane interactions) could be experimentally altered through the intramammary infusion of solutions with different osmotic potentials. These observations support the suggestion that osmotic conditions may influence exocytosis of milk serum. 相似文献
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Summary Whether both casein and noncasein (serum or whey) proteins of milk are contained within the same secretory vesicles of milk secreting mammary epithelial cells was explored. Antibodies to a major casein and to -lactalbumin of rat milk were localized in thin sections with colloidal gold-conjugated second antibodies. Antibodies to the casein component bound to an antigen present within lumina of Golgi apparatus cisternae and within secretory vesicles. This antigen was also recognized in structures within secretory vesicles and within alveolar lumina which were ultrastructurally identified as casein micelles. Antigens recognized by antibodies to -lactalbumin also were present in Golgi apparatus cisternae and within secretory vesicles. Both anti-casein and anti--lactalbumin antibodies recognized antigens within the same secretory vesicles. These observations show that one major noncasein protein of rat's milk is present in casein-containing secretory vesicles. 相似文献
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Wanda Peczyńska-Czoch Jarosław Osiadacz Łukasz Kaczmarek Tomasz Żal 《Biotechnology letters》1996,18(2):123-128
Summary Microbial transformation of cytotoxic 5,11-dimethyl-5H-indolo[2,3-b]quinoline (a compound displaying antitumor activity and affecting the activity of calf thymus DNA topoisomerase II) was performed by the Rhizopus arrhizus strain and yielded a 9-hydroxy derivative. The metabolite obtained displayed a stronger cytotoxity against KB cells than the parent compound (ID50=0.001 mol/mL), and stimulated also the formation of calf thymus topoisomerase II mediated pSP65 DNA cleavage in vitro at the concentration of 3 M. Being analogous to 9-hydroxyellipticine (which is an antitumor alkaloid), this novel indolo[2,3-b] quinoline derivative can be regarded as a novel potential antitumor agent. 相似文献
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Łukasz Wejnerowski Ewa Poniecka Jakub Buda Piotr Klimaszyk Agnieszka Piasecka Marcin Krzysztof Dziuba Gianmarco Mugnai Nozomu Takeuchi Krzysztof Zawierucha 《Journal of phycology》2023,59(5):939-949
Cryoconite, the dark sediment on the surface of glaciers, often aggregates into oval or irregular granules serving as biogeochemical factories. They reduce a glacier's albedo, act as biodiversity hotspots by supporting aerobic and anaerobic microbial communities, constitute one of the organic matter (OM) sources on glaciers, and are a feeder for micrometazoans. Although cryoconite granules have multiple roles on glaciers, their formation is poorly understood. Cyanobacteria are ubiquitous and abundant engineers of cryoconite hole ecosystems. This study tested whether cyanobacteria may be responsible for cryoconite granulation as a sole biotic element. Incubation of Greenlandic, Svalbard, and Scandinavian cyanobacteria in different nutrient availabilities and substrata for growth (distilled water alone and water with quartz powder, furnaced cryoconite without OM, or powdered rocks from glacial catchment) revealed that cyanobacteria bind mineral particles into granules. The structures formed in the experiment resembled those commonly observed in natural cryoconite holes: they contained numerous cyanobacterial filaments protruding from aggregated mineral particles. Moreover, all examined strains were confirmed to produce extracellular polymeric substances (EPS), which suggests that cryoconite granulation is most likely due to EPS secretion by gliding cyanobacteria. In the presence of water as the only substrate for growth, cyanobacteria formed mostly carpet-like mats. Our data empirically prove that EPS-producing oscillatorialean cyanobacteria isolated from the diverse community of cryoconite microorganisms can form granules from mineral substrate and that the presence of the mineral substrate increases the probability of the formation of these important and complex biogeochemical microstructures on glaciers. 相似文献