全文获取类型
收费全文 | 2727篇 |
免费 | 182篇 |
出版年
2023年 | 15篇 |
2022年 | 13篇 |
2021年 | 19篇 |
2020年 | 20篇 |
2019年 | 34篇 |
2018年 | 91篇 |
2017年 | 68篇 |
2016年 | 120篇 |
2015年 | 188篇 |
2014年 | 147篇 |
2013年 | 258篇 |
2012年 | 191篇 |
2011年 | 146篇 |
2010年 | 141篇 |
2009年 | 119篇 |
2008年 | 105篇 |
2007年 | 85篇 |
2006年 | 75篇 |
2005年 | 91篇 |
2004年 | 72篇 |
2003年 | 57篇 |
2002年 | 56篇 |
2001年 | 65篇 |
2000年 | 65篇 |
1999年 | 56篇 |
1998年 | 21篇 |
1997年 | 17篇 |
1996年 | 16篇 |
1995年 | 22篇 |
1994年 | 11篇 |
1993年 | 12篇 |
1992年 | 45篇 |
1991年 | 35篇 |
1990年 | 32篇 |
1989年 | 29篇 |
1988年 | 25篇 |
1987年 | 30篇 |
1986年 | 26篇 |
1985年 | 25篇 |
1984年 | 20篇 |
1983年 | 18篇 |
1982年 | 18篇 |
1980年 | 13篇 |
1975年 | 11篇 |
1974年 | 13篇 |
1973年 | 16篇 |
1972年 | 20篇 |
1970年 | 16篇 |
1968年 | 11篇 |
1965年 | 20篇 |
排序方式: 共有2909条查询结果,搜索用时 31 毫秒
1.
2.
3.
4.
Background
With increasing computer power, simulating the dynamics of complex systems in chemistry and biology is becoming increasingly routine. The modelling of individual reactions in (bio)chemical systems involves a large number of random events that can be simulated by the stochastic simulation algorithm (SSA). The key quantity is the step size, or waiting time, τ, whose value inversely depends on the size of the propensities of the different channel reactions and which needs to be re-evaluated after every firing event. Such a discrete event simulation may be extremely expensive, in particular for stiff systems where τ can be very short due to the fast kinetics of some of the channel reactions. Several alternative methods have been put forward to increase the integration step size. The so-called τ-leap approach takes a larger step size by allowing all the reactions to fire, from a Poisson or Binomial distribution, within that step. Although the expected value for the different species in the reactive system is maintained with respect to more precise methods, the variance at steady state can suffer from large errors as τ grows. 相似文献5.
The cell cycle modulated glycoprotein GP115 is one of the major yeast proteins containing glycosylphosphatidylinositol 总被引:11,自引:0,他引:11
The cell cycle modulated protein gp115 (115 kDa, isoelectric point about 4.8-5) of Saccharomyces cerevisiae undergoes various post-translational modifications. It is N-glycosylated during its maturation along the secretory pathway where an intermediary precursor of 100 kDa (p100), dynamically related to the mature gp115 protein, is detected at the level of endoplasmic reticulum. Moreover, we have shown by the use of metabolic labeling with [35S]methionine, [3H]palmitic acid and myo-[3H]inositol combined with high resolution two-dimensional gel electrophoresis and immunoprecipitation with a specific antiserum, that gp115 is one of the major palmitate- and inositol-containing proteins in yeast. These results, and the susceptibility of gp115 to phosphatidylinositol-specific phospholipase C treatment strongly indicate that gp115 contains the glycosylphosphatidylinositol (GPI) structure as membrane anchor domain. The two-dimensional analysis of the palmitate- and inositol-labeled proteins has also allowed the characterization of other polypeptides which possibly contain a GPI structure. 相似文献
6.
Rita Padányi Yuning Xiong Géza Antalffy Krisztina Lór Katalin Pászty Emanuel E. Strehler ágnes Enyedi 《The Journal of biological chemistry》2010,285(41):31704-31712
The membrane localization of the plasma membrane Ca2+-ATPase isoform 2 (PMCA2) in polarized cells is determined by alternative splicing; the PMCA2w/b splice variant shows apical localization, whereas the PMCA2z/b and PMCA2x/b variants are mostly basolateral. We previously reported that PMCA2b interacts with the PDZ protein Na+/H+ exchanger regulatory factor 2 (NHERF2), but the role of this interaction for the specific membrane localization of PMCA2 is not known. Here we show that co-expression of NHERF2 greatly enhanced the apical localization of GFP-tagged PMCA2w/b in polarized Madin-Darby canine kidney cells. GFP-PMCA2z/b was also redirected to the apical membrane by NHERF2, whereas GFP-PMCA2x/b remained exclusively basolateral. In the presence of NHERF2, GFP-PMCA2w/b co-localized with the actin-binding protein ezrin even after disruption of the actin cytoskeleton by cytochalasin D or latrunculin B. Surface biotinylation and fluorescence recovery after photobleaching experiments demonstrated that NHERF2-mediated anchorage to the actin cytoskeleton reduced internalization and lateral mobility of the pump. Our results show that the specific interaction with NHERF2 enhances the apical concentration of PMCA2w/b by anchoring the pump to the apical membrane cytoskeleton. The data also suggest that the x/b splice form of PMCA2 contains a dominant lateral targeting signal, whereas the targeting and localization of the z/b form are more flexible and not fully determined by intrinsic sequence features. 相似文献
7.
8.
9.
10.