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111.
Salinity tolerance of Kosteletzkya virginica. I. Shoot growth, ion and water relations 总被引:5,自引:2,他引:3
Abstract. Kosteletzkya virginica (L.) Presl., a dicotyledonous halophyte native to brackish tidal marshes, was grown on nutrient solution containing 0. 85, 170 or 255 mol m-3 NaCl, and the effects of external salinity on shoot growth and ion content of individual leaves were studied in successive harvests. Growth was stimulated by 85 mol m-3 NaCl and was progressively reduced at the two higher salinities. Growth suppression at high salinity resulted principally from decreased leaf production and area, not from accelerated leaf death. As is characteristic of halophytic dicots. K. virginica accumulated inorganic ions in its leaves, particularly Na+ and K+ . However, the Na+ concentration of individual leaves did not increase with time, but remained constant or even declined, seeming to be well-coordinated with changes in water content. A striking feature of the ion composition of salinized plants was the development of a dramatic gradient in sodium content, with Na+ partitioned away from the most actively growing leaves. Salt-treated plants exhibited a strong potassium affinity, with foliar K+ levels higher in salinized plants than unsalinized plants after an initial decrease. These results suggest that selective uptake and transport, foliar compartmentation of Na+ and K+ in opposite directions along the shoot axis, and the regulation of leaf salt loads over time to prevent build-up of toxic concentrations are whole-plant features which enable K. virginica to establish favourable K+ -Na+ relations under saline conditions. 相似文献
112.
Salinity tolerance of Kosteletzkya virginica. II. Root growth, lipid content, ion and water relations 总被引:1,自引:0,他引:1
Abstract. Kosteletzkya virginica (L.) Presl., a dicot halophyte native to brackish tidal marshes, was grown on nutrient solution containing 0. 85, 170 or 255 mol m 3 NaCl, and the effects of external salinity on root growth, ion and water levels, and lipid content were examined in successive harvests. Root growth paralleled shoot growth trends, with some enhancement observed at 85 mol m 3 NaCl and a reduction noted at the higher salinities. Root Na+ content increased with increasing external NaCl, but remained constant with time for each treatment. K+ content, although lower in salt-grown plants after 14 d salinization, subsequently increased to levels comparable to unsalinized plants. A strong K+ affinity was reflected in the increased K+ /Na+ selectivity of salt-grown plants and by their low Na+ /K+ ratios. Cl levels rose in salinized plants and values were double or more those for Na+ , indicating the possibility of a sodium-excluding mechanism in roots. Root phospholipids and sterols, principal membrane constituents, were maintained or elevated and the free sterol/phospholipids ratio increased in salinized K. virginica plants, suggesting retention of overall membrane structure and decreased permeability. This response, considered in light of root calcium maintenance and high potassium levels, suggests that salinity-induced changes in membrane lipid composition may be important in preventing K+ leakage from cells. 相似文献
113.
Effects of metals on enzyme activity in plants 总被引:16,自引:0,他引:16
Abstract. Uptake of phytotoxic amounts of metal by higher plants or algae can result in inhibition of several enzymes, and in increase in activity (= induction) of others. Two mechanisms of enzyme inhibition predominate: (1) binding of the metal to sulphydryl groups, involved in the catalytic actionor structural integrity of enzymes, and (2) deficiency of an essential metal in metalloproteins or metal-protein complexes, eventually combined with substitution of the toxic metal for the deficient element. Metal accumulation in the cellular compartment of the enzyme is a prerequisite for enzyme inhibition in vivo. The induction of some enzymes is considered to play a significant role in the stress metabolism, induced by metal phytotoxicity. Peroxidase induction is likely to be related to oxidative reactions at the biomembrane; several enzymes of the intermediary metabolism might be stimulated to compensate for metal-sensitive photosynthetic reactions. The induction of enzymes and metal-specific changes in isoperoxidase pattern can be used as diagnostic criteria to evaluate the phytotoxicity of soils, contaminated by several metals. Lines for future research on metal phytotoxicity are proposed, involving the study of inhibition and induction of enzymes at the different cell membranes (especially the plasmamembrane) in vivo. 相似文献
114.
Vimal Kishore 《Biological trace element research》1990,25(2):123-135
Concentrations of copper, zinc, and iron were analyzed and compared in a number of tissues of adjuvant arthritic rats following 22 d of chronic treatment (per os) with either vehicle, aspirin or copper aspirinate, at doses of 100 mg/kg, 200 mg/kg, or 400 mg/kg. Such chronic treatment resulted in a negative balance in copper, zinc, and iron in many tissues. Among the tissues examined, liver and kidney exhibited the greatest changes in metal concentrations; brain and skeletal muscle exhibited the least. Arthritis-induced changes in the concentrations of all three metals in the liver were reversed upon treatment with aspirin. Treatment with copper aspirinate, on the other hand, resulted in an extremely high accumulation of copper in the liver. Arthritis-induced changes in copper, zinc, and iron concentrations in the pancreas and copper concentration in the plasma were generally not reversed upon treatment with either aspirin or copper aspirinate. Among the three metals examined, the degree of change observed as a result of drug treatments was greatest for iron and least for zinc. Finally, it appeared that the effects of aspirin and copper aspirinate on tissue metal concentrations were independent of the antiarthritic effects of these compounds. 相似文献
115.
Putzer V. M. de Zwaan A. Wieser W. 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》1990,159(6):707-715
Summary Anoxia tolerance, glycogen degradation, free amino acid pool, adenylate energy charge and the accumulation and excretion of end products were monitored inLumbriculus variegatus Müller throughout 48 h of anoxia. A transition period lasting about 4 h could be distinguished from subsequent events during which malate, present in high amounts in the resting animals, is utilized, probably by conversion to succinate. Up to the 12th hour of anoxia there is an increase in concentration of free amino acids, except aspartate. Glutamate increases rapidly during the first half hour but decreases thereafter. Beginning with the second hour of anoxia the alanine concentration increases at the same rate glutamate concentration decreases, but the source of nitrogen during the first hour is unknown. It is argued that the nitrogen required for the synthesis of some of the amino acids is ultimately derived from proteolysis. After about 3 h of anoxia propionate and acetate are synthesized. At first these acids accumulate in the tissues, but after 4–6 h they are excreted into the surrounding medium. Acetate is excreted over the whole experimental period at a constant rate, whereas the excretion rate of propionate decreases slowly with time. The propionate/acetate ratio is in excess of 2. Classic malate dismutation is by far the most important mechanism in the maintenance of redox balance. Depletion of glycogen stores appears to play an important role in determining anoxic survival time. Due to extremely low activity of PEPCK the ratio of the specific activities of PK and PEPCK is very high. Further, the kinetic properties of pyruvate kinase do not support the assumption of a shift of the glycolytic carbon flow at the PEP level.Abbreviations
PK
Pyruvate kinase
-
PEPCK
phosphoenolpyruvate carboxykinase
-
PEP
phospho(enol)pyruvate
-
FBP
fructose-1,6-bisphosphate
-
AEC
adenylate energy charge
-
EMP-scheme
Embden-Meyerhof-Parnas scheme of glycolysis
-
f
w
fresh body weight
-
dw
dry body weight 相似文献
116.
Tissa Senaratna Bryan D. McKersie Stephen R. Bowley 《In vitro cellular & developmental biology. Plant》1990,26(1):85-90
Summary The use of somatic embryos from cell culture systems in the clonal propagation of plants would be greatly facilitated if the
somatic embryos could be dried and stored in a dormant state similar to true seeds. A cell culture system was developed for
alfalfa (Medicago sativa L.) line RL34 which gave high yields of somatic embryos in an approximately synchronized pattern. These somatic embryos were
treated with abscisic acid (ABA) at the cotyledonary stage of development to induce desiccation tolerance. With no visual
preselection, approximately 60% of the dried embryos converted into plants upon reimbibition. When high quality embryos were
selected prior to drying, 90 to 100% conversion rates were observed. The timing of the application of ABA in terms of embryo
development was critical with an optimum being at cotyledonary stage spanning approximately 4 days; thus, synchronized embryo
development is required for optimal expression in bulk samples. The vigor of the seedlings from dried somatic embryos was
greater than those from embryos which had not been dried, but remained substantially lower than those from true seeds. 相似文献
117.
Abstract. Regeneration levels, size class distributions and a nearest neighbour technique were used to describe apreliminary dynamics frameworkfortheplateauforests. Taken over a large area, most of the canopy dominants have a negative exponential distribution of stems > 10 cm dbh per size class. In small (0.04 ha) plots, if present as more than one stem, most species are present as both canopy (> 25 cm dbh) and understorey (10–15 cm dbh) individuals. The canopy dominants maintain their rank in the bank of advance regeneration (> 5 cm height < 10 cm dbh). However, relative numbers of all regeneration of most canopy individuals are not strongly correlated with canopy closure or local abundance of conspecific adults. The mean area of nearest neighbour polygons of canopy individuals around saplings of the more common species are small. In conclusion, most species appearto be shade tolerant and locally persistent conferring a fine grain on this forest. We support recent questioning of universality of the gaps/non gaps paradigm. 相似文献
118.
Iris D. Zellmer 《Hydrobiologia》1996,319(2):87-92
UV-B (290 nm) tolerance of Daphnia pulex, conditioned to four different food levels (Chlorophyta), was tested under standardized conditions with an artificial radiation source. Parameters measured were survival, percentage of egg bearing Daphnia and the number of juveniles produced after irradiation. UV-B tolerance of Daphnia pulex was found to be significantly improved with increasing food concentrations at all three parameters. The impact of the four different food concentrations on the photoreactivation system was tested with simultanous UV-B and white-light irradiation of Daphnia. Survival rate improved significantly with increasing food levels compared to solely UV-B irradiation. Photoreactivation had no effect on the reproductive parameters. 相似文献
119.
A survey of Connecticut lakes and rivers revealed the presence of 7 species of freshwater sponge: Spongilla lacustris, Ephydatia muelleri, Eunapius fragilis, Anheteromeyenia ryderi, A. argyrosperma, Corvomeyenia carolinensis, and Corvospongilla novaeterrae in order of decreasing frequency of occurrence. Corvomeyenia carolinensis has not been reported previously beyond its type locality in South Carolina. In addition, microscleres of Spongilla lacustris, Anheteromeyenia-like megascleres, Ephydatia muelleri-like megascleres, and smooth megascleres (amphioxeas), which could not be assigned to a particular species, were found in surface sediments from lake cores. Spongilla lacustris inhabiting small rivers produced brown, thick-capsuled gemmules during the summer and yellow, thin-capsuled gemmules during the fall. The thick-capsuled gemmules, but not the thin-capsuled gemmules, are tolerant of desiccation; and populations of Spongilla lacustris and Ephydatia muelleri survived severe drying of their habitats during the summer. 相似文献
120.