New scoring system combining the FIB-4 index and cytokeratin-18 fragments for predicting steatohepatitis and liver fibrosis in patients with nonalcoholic fatty liver disease

Purpose: To establish a new scoring system as a noninvasive tool for predicting steatohepatitis and liver fibrosis in patients with nonalcoholic fatty liver disease (NAFLD).

Methods: A total of 170 patients histologically diagnosed with nonalcoholic steatohepatitis (NASH) (n?=?130) or nonalcoholic fatty liver (NAFL) (n?=?40) were enrolled. We analyzed receiver operating characteristic (ROC) curves and performed multivariate analysis to predict steatohepatitis and liver fibrosis.

Results: Multivariate analysis showed that cytokeratin-18 fragment (CK18-F) levels (≥278?U/L) (odds ratio [OR], 4.46; 95% confidence interval [CI], 1.42–14.00; p?=?0.010) and the FIB-4 index (≥1.46) (OR, 4.54; 95% CI, 1.93–29.50; p?=?0.004) were independently associated with prediction of NASH. We then established a new scoring system (named the FIC-22 score) for predicting NASH using CK18-F levels and FIB-4 index. The areas under the ROC curve (AUROCs) of the FIC-22 score and NAFIC score were 0.82 (95% CI, 0.75–0.89) and 0.71 (95% CI, 0.62–0.78) (p?=?0.044). Additionally, the AUROC of the FIC-22 score for predicting the presence of fibrosis (F?≥?1) was 0.78 (95% CI, 0.70–0.85).

Conclusions: In patients with NAFLD, the FIC-22 score had high predictive accuracy not only for steatohepatitis but also for the presence of liver fibrosis.     

脓毒症患者炎性因子、凝血功能与APACHEⅡ评分和预后的关系

目的:研究脓毒症患者炎性因子、凝血功能与急性生理学和慢性健康状况Ⅱ(APACHEⅡ)评分和预后的关系。方法:选取2017年1月至2018年1月我院收治的脓毒症患者150例为脓毒症组,所有患者根据预后结果分为死亡组(n=49)和存活组(n=101),选择同期在我院住院的非脓毒症患者98例为非脓毒症组,比较脓毒症组与非脓毒症组患者炎性因子、凝血功能指标水平及APACHEⅡ评分,同时比较死亡组和存活组患者炎性因子、凝血功能指标水平及APACHEⅡ评分,并分析脓毒症患者APACHEⅡ评分与炎性因子、凝血功能指标的相关性。结果:脓毒症组患者降钙素原(PCT)、活化部分凝血酶原时间(APTT)、凝血酶原时间(PT)水平及APACHEⅡ评分均高于非脓毒症组,血小板计数(PLT)低于非脓毒症组(P0.05),两组C-反应蛋白(CRP)比较差异无统计学意义(P0.05)。死亡组患者PCT、APTT、PT水平及APACHEⅡ评分均高于存活组,PLT水平低于存活组(P0.05),两组CRP比较差异无统计学意义(P0.05)。经Spearman相关性分析结果显示,脓毒症患者APACHEⅡ评分与PCT、APTT、PT均呈正相关关系,与PLT呈负相关关系(P0.05),与CRP无相关性(P0.05)。结论:脓毒症患者PCT、APTT、PT水平明显上升,PLT水平明显下降,且均与患者的APACHEⅡ评分密切相关,临床可通过调节炎症因子水平及凝血功能指标从而改善患者的病情和预后。     

丁苯酞软胶囊对急性脑梗死患者ROS、T-AOC、炎症因子及神经功能的影响

目的:探讨丁苯酞软胶囊对急性脑梗死患者活性氧(reactive oxygen species,ROS)、总抗氧化能力(total antioxidant capacity,T-AOC)、炎症因子及神经功能的影响。方法:将2016年7月至2017年7月我院收治的急性脑梗死患者100例随机分为对照组(n=50)和观察组(n=50)。对照组患者给予常规对症治疗,观察组患者在对照组的基础上加用丁苯酞软胶囊。两组连续治疗14天后,比较治疗前后两组血清ROS、T-AOC、炎症因子水平及神经功能的变化及治疗后两组的临床疗效。结果:治疗前,两组血清ROS、T-AOC、高敏C反应蛋白(high sensitive C reaction protein,hs-CRP)、肿瘤坏死因子-α(tumor necrosis factor,TNF-α)、白细胞介素-6(interleukin-6,IL-6)水平、美国国立卫生研究院卒中量表(National Institute of Health stroke scale)评分比较差异均无统计学意义(P0.05)。与本组治疗前比较,两组治疗后血清T-AOC水平显著升高,且观察组显著高于对照组(P0.05);对照组治疗后血清ROS水平显著高于治疗前(P0.05),观察组患者治疗前后的血清ROS水平相比差异无统计学意义(P0.05),且观察组治疗后ROS水平显著低于对照组(P0.05);两组血清hs-CRP、TNF-α、IL-6水平、NHISS评分均显著低于治疗前(P0.05),且观察组以上指标均显著低于对照组(P0.05);观察组总有效率为82%,明显优于对照组的52%(P0.05)。结论:在常规治疗的基础上加用丁苯酞软胶囊可有效降低急性脑梗死患者血清炎症因子水平,提高机体总抗氧化能力,促进神经功能的修复,提高临床治疗效果     

Identification of potent lysophosphatidic acid receptor 5 (LPA5) antagonists as potential analgesic agents

Lysophosphatidic acid (LPA) plays an important role in a variety of cellular functions. In particular, LPA5 receptor is highly expressed in spinal cord and dorsal root ganglion, which are associated with pain. This fact prompted us to hypothesize that LPA5 antagonists show analgesic effects. To search for potent LPA5 antagonists with blood brain barrier (BBB) permeability, we conducted high throughput screening (HTS). In HTS campaign, we found a 2H-isoquinoline-1-one scaffold showing antagonistic activity against LPA5 and synthesized a series of 2H-isoquinoline-1-one derivatives and evaluated their LPA5 activities. Among these compounds, compound 7e showed potent LPA5 activity with an IC₅₀ value of 0.12?μM, and acceptable BBB permeability. Furthermore, it showed effective analgesic effect in a chronic constriction injury rat model. Therefore, 7e may have a potential as novel pain therapeutic approach.     

高效液相色谱法对农业样品中氨基酸含量的测定

介绍了采用一种新型的国产氨基酸色谱柱ＹＷＧＡ—Ａ柱,对与农业有关的多种样品进行氨基酸测定的方法。这种色谱柱可使全部氨基酸得到完全的分离,且柱效高,柱压低,价格便宜。农业样品的品种多,比较复杂,文中还对不同样品的前处理做了简要叙述。氨基酸的分析方法采用的是柱前衍生ＯＰＡ法,还对此方法测定中的一些问题进行了讨论。