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971.
Gap junctions (GJs) traverse apposing membranes of neighboring cells to mediate intercellular communication by passive diffusion of signaling molecules. We have shown previously that cells endocytose GJs utilizing the clathrin machinery. Endocytosis generates cytoplasmic double-membrane vesicles termed annular gap junctions or connexosomes. However, the signaling pathways and protein modifications that trigger GJ endocytosis are largely unknown. Treating mouse embryonic stem cell colonies – endogenously expressing the GJ protein connexin43 (Cx43) – with epidermal growth factor (EGF) inhibited intercellular communication by 64% and activated both, MAPK and PKC signaling cascades to phosphorylate Cx43 on serines 262, 279/282, and 368. Upon EGF treatment Cx43 phosphorylation transiently increased up to 4-fold and induced efficient (66.4%) GJ endocytosis as evidenced by a 5.9-fold increase in Cx43/clathrin co-precipitation. 相似文献
972.
Marion Chartier Marc Gibernau Susanne S. Renner 《Evolution; international journal of organic evolution》2014,68(5):1533-1543
Most plant–pollinator interactions are mutualistic, involving rewards provided by flowers or inflorescences to pollinators. Antagonistic plant–pollinator interactions, in which flowers offer no rewards, are rare and concentrated in a few families including Araceae. In the latter, they involve trapping of pollinators, which are released loaded with pollen but unrewarded. To understand the evolution of such systems, we compiled data on the pollinators and types of interactions, and coded 21 characters, including interaction type, pollinator order, and 19 floral traits. A phylogenetic framework comes from a matrix of plastid and new nuclear DNA sequences for 135 species from 119 genera (5342 nucleotides). The ancestral pollination interaction in Araceae was reconstructed as probably rewarding albeit with low confidence because information is available for only 56 of the 120–130 genera. Bayesian stochastic trait mapping showed that spadix zonation, presence of an appendix, and flower sexuality were correlated with pollination interaction type. In the Araceae, having unisexual flowers appears to have provided the morphological precondition for the evolution of traps. Compared with the frequency of shifts between deceptive and rewarding pollination systems in orchids, our results indicate less lability in the Araceae, probably because of morphologically and sexually more specialized inflorescences. 相似文献
973.
Piedad Gallego Luisa Martin Antonio Blazquez Hilario Guerra Nieves Villalobos 《Journal of plant physiology》2014
The legume Medicago arborea L. is very interesting as regards the regeneration of marginal arid soils. The problem is that it does not have a good germinative yield. It was therefore decided to regenerate via somatic embryogenesis and find a marker of embryogenic potential. In this study, peroxidase activity was evaluated in non-embryogenic and embryogenic calli from M. arborea L. A decrease in soluble peroxidase activity is observed in its embryonic calli at the time at which the somatic embryos begin to appear. This activity is always lower in embryonic calli than in non-embryonic ones (unlike what happens in the case of wall-bound peroxidases). These results suggest that peroxidases can be considered to be enzymes involved in somatic embryogenesis in M. arborea. In addition, isozyme analyses were carried out on protein extracts using polyacrylamide gel electrophoresis. The band called P5 was detected only in embryogenic cultures at very early stages of development. This band was digested with trypsin and analyzed using linear ion trap (LTQ) mass spectrometer. In P5 isoform a peroxidase-l-ascorbate peroxidase was identified. It can be used as a marker that allows the identification of embryological potential. 相似文献
974.
975.
Bean Yellow Mosaic Virus in Soybean from Argentina 总被引:1,自引:0,他引:1
Claudia Nome Irma G. Laguna Patricia Rodríguez Pardina 《Journal of Phytopathology》2014,162(5):322-325
976.
977.
Genomics and molecular breeding in lesser explored pulse crops: Current trends and future opportunities 总被引:1,自引:0,他引:1
Abhishek Bohra Uday Chand Jha P.B. Kavi Kishor Shailesh Pandey Narendra P. Singh 《Biotechnology advances》2014
Pulses are multipurpose crops for providing income, employment and food security in the underprivileged regions, notably the FAO-defined low-income food-deficit countries. Owing to their intrinsic ability to endure environmental adversities and the least input/management requirements, these crops remain central to subsistence farming. Given their pivotal role in rain-fed agriculture, substantial research has been invested to boost the productivity of these pulse crops. To this end, genomic tools and technologies have appeared as the compelling supplement to the conventional breeding. However, the progress in minor pulse crops including dry beans (Vigna spp.), lupins, lablab, lathyrus and vetches has remained unsatisfactory, hence these crops are often labeled as low profile or lesser researched. Nevertheless, recent scientific and technological breakthroughs particularly the next generation sequencing (NGS) are radically transforming the scenario of genomics and molecular breeding in these minor crops. NGS techniques have allowed de novo assembly of whole genomes in these orphan crops. Moreover, the availability of a reference genome sequence would promote re-sequencing of diverse genotypes to unlock allelic diversity at a genome-wide scale. In parallel, NGS has offered high-resolution genetic maps or more precisely, a robust genetic framework to implement whole-genome strategies for crop improvement. As has already been demonstrated in lupin, sequencing-based genotyping of the representative sample provided access to a number of functionally-relevant markers that could be deployed straight away in crop breeding programs. This article attempts to outline the recent progress made in genomics of these lesser explored pulse crops, and examines the prospects of genomics assisted integrated breeding to enhance and stabilize crop yields. 相似文献
978.
979.
2013年4—7月,利用红外相机在四川王朗自然保护区研究了血雉(Ithaginis cruentus)在繁殖初期和育雏期的日活动模式和集群行为。结果表明,血雉具有明显的昼行性活动规律,繁殖初期的活动高峰为6:00—14:00(n=42,P=84.00%),育雏期的活动高峰为6:00—12:00(n=111,P=60.33%),次高峰为12:00—18:00(n=63,P=34.24%)。繁殖初期记录了50群76只血雉,雌雄性比为1∶2.41,集群大小为(1.52±0.68)只/群,以孤雄(n=23,P=46.00%)和混合群(n=29,P=30.00%)为主。育雏期记录了184群321只血雉,雌雄性比为1∶1.81,集群大小为(1.74±1.21)只/群,以孤雄(n=75,P=40.76%)和家庭群(n=51,P=27.72%)为主。在繁殖初期和育雏期,不同集群类型出现的频率差异均极显著(繁殖初期:χ2=17.680,df=3,P〈0.01;育雏期:χ2=79.478,df=4,P〈0.01)。血雉的集群性较强,即使在以家庭生活为主的育雏期也常集群活动。 相似文献
980.
Lawrence Tannenbaum 《人类与生态风险评估》2014,20(2):479-496
Upland birds that display grit ingestion behavior are potentially at risk of detrimental effects and death from lead poisoning at trap and skeet ranges and other areas where vast quantities of spent lead shot pellets abound. Because commonly cited force-feeding pellet exposure studies deviate from true field conditions, their results may not reflect true risks faced by upland birds. In particular, studies that use new shot pellets and administer more pellets than would be reasonably ingested, critically interfere with the understanding of actualized pellet exposures. In this study, northern bobwhites (Colinus virginianus), a frequent test species in shot pellet research, were dosed in an ecologically-relevant manner (i.e., with spent shot and with no more than three pellets). Notably, the 56-day post-dosing observation period, during which a battery of physiological measures were recorded, exceeded that of related studies. Despite a sustained suppression of a lead poisoning indicator, the data suggest upland birds can withstand spent shot pellet exposures. Data detected a survivorship ≥95%, absence of illness, demonstrated tolerance for extremely high blood lead concentrations, and unaffected blood parameters. In conjunction with ecological considerations (e.g., spatial scale and animal behavior), concern about bird population losses from the incidental ingestion of spent shot pellets is potentially overstated. 相似文献