Multi-Scale Assessment of Male Northern Yellow Bat Roost Selection

Knowledge of roost selection by northern yellow bats (Lasiurus intermedius) is limited to a small number of known roost locations. Yet knowledge of basic life history is fundamental to understanding past response to anthropogenic change and to predict how species will respond to future environmental change. Therefore, we examined male northern yellow bat roost selection on 2 Georgia, USA, barrier islands with different disturbance histories. Sapelo Island has a history of extensive disturbance and is dominated by pine (Pinus spp.) forests; Little Saint Simons Island has a limited disturbance history with maritime oak (Quercus spp.) forest as the dominant cover type. From March–July 2012 and 2013, we radio-tracked 35 adult male northern yellow bats to diurnal roosts and modeled roost characteristics at the plot and landscape scales. We located 387 roosts, of which 95% were in Spanish moss (Tillandsia usneoides) hanging in hardwood trees. On both islands, bats selected roost trees with larger diameters than surrounding trees and selected roost locations with greater open flight space (i.e., low midstory clutter) underneath. Roosts were located farther from open areas on Sapelo and closer to fresh water on Little Saint Simons compared to random locations. Lower availability of hardwood forest on Sapelo may have resulted in small-scale roost site selection (i.e., plot level) despite potential increased costs of commuting to water and open areas for foraging. In contrast, greater availability of hardwood forest on Little Saint Simons likely allowed selection of roosts closer to fresh water, which provides foraging and drinking opportunities. Our results indicate that mature hardwood trees in areas with low midstory clutter are important in male northern yellow bat roost selection, but landscape-level features have varying influences on roost selection, likely as a result of differences in disturbance history. Therefore, management will differ depending on the landscape context. Further research is needed to examine roost selection by females, which may have different habitat requirements. © 2020 The Wildlife Society.     

Transmission of peanut yellow spot virus (PYSV) by Thrips,Scirtothrips dorsalis Hood in groundnut †

Peanut yellow spot virus (PYSV) was efficiently transmitted by Scirtothrips dorsalis Hood in groundnut. Larvae could acquire the virus in 30 min and the maximum percentage transmission of 43.8% by individual insects resulted following two days AAP. Single adult Thrip transmitted the virus after minimum IAP of 30 minutes. The percentage transmission (33.3%) increased linearly with an increase in IAP up to 1.5 days and maximum up to 55 h of IAP (36.1%). PYSV persistently transmitted more than 75% of their life span.     

Study of resistance components of some promising wheat lines to yellow rust disease in the seedling stage

The most reliable method to control the wheat yellow rust disease is cultivation of resistance cultivars. To provide resistance, it is necessary to be aware of the amount and the quality of pathogenesis of disease factors and resistant specifications. In this study, 82 wheat promising lines with Bolani susceptible cultivar in randomised complete block design were tested in the seedling stage. This experiment was carried out in greenhouse condition, and it was assessed by two races: 166E254A+Yr27+ and 6E150A+, which were more and less pathogenic, respectively. The attributes of resistance were measured for infection type (IT), latent period (LP), pustule size (PS) and density. Results of variance analysis relating two races between wheat genotypes for these four attributes of resistance showed that there is a difference in the probability at 1% level. The statistical analyses for these components of resistance indicated that there is negative and high solidarity between IT and LP, and also among the number and density of pustules. The correlation between IT and LP and both races were -0.90 and -0.98, respectively. Cluster analysis of lines to each race was classified as resistant, semi-resistant and susceptible. The first group of the resistant lines were 27 lines in which their ITs of 0–2, mean LP of 18?days PS of 2.8 and pustule density of 1.1 were recorded.     

Physical mapping and cloning of RAD56

Here we report the physical mapping of the rad56-1 mutation to the NAT3 gene, which encodes the catalytic subunit of the NatB N-terminal acetyltransferase in Saccharomyces cerevisiae. Mutation of RAD56 causes sensitivity to X-rays, methyl methanesulfonate, zeocin, camptothecin and hydroxyurea, but not to UV light, suggesting that N-terminal acetylation of specific DNA repair proteins is important for efficient DNA repair.     

Functional analysis and tissue-differential expression of four FAD2 genes in amphidiploid Brassica napus derived from Brassica rapa and Brassica oleracea

Fatty acid desaturase 2 (FAD2), which resides in the endoplasmic reticulum (ER), plays a crucial role in producing linoleic acid (18:2) through catalyzing the desaturation of oleic acid (18:1) by double bond formation at the delta 12 position. FAD2 catalyzes the first step needed for the production of polyunsaturated fatty acids found in the glycerolipids of cell membranes and the triacylglycerols in seeds. In this study, four FAD2 genes from amphidiploid Brassica napus genome were isolated by PCR amplification, with their enzymatic functions predicted by sequence analysis of the cDNAs. Fatty acid analysis of budding yeast transformed with each of the FAD2 genes showed that whereas BnFAD2-1, BnFAD2-2, and BnFAD2-4 are functional enzymes, and BnFAD2-3 is nonfunctional. The four FAD2 genes of B. napus originated from synthetic hybridization of its diploid progenitors Brassica rapa and Brassica oleracea, each of which has two FAD2 genes identical to those of B. napus. The BnFAD2-3 gene of B. napus, a nonfunctional pseudogene mutated by multiple nucleotide deletions and insertions, was inherited from B. rapa. All BnFAD2 isozymes except BnFAD2-3 localized to the ER. Nonfunctional BnFAD2-3 localized to the nucleus and chloroplasts. Four BnFAD2 genes can be classified on the basis of their expression patterns.     

An estuarine species of the alga Vaucheria (Xanthophyceae) displays an increased capacity for turgor regulation when compared to a freshwater species

Turgor regulation is the process by which walled organisms alter their internal osmotic potential to adapt to osmotic changes in the environment. Apart from a few studies on freshwater oomycetes, the ability of stramenopiles to turgor regulate has not been investigated. In this study, turgor regulation and growth were compared in two species of the stramenopile alga Vaucheria, Vaucheria erythrospora isolated from an estuarine habitat, and Vaucheria repens isolated from a freshwater habitat. Species were identified using their rbcL sequences and respective morphologies. Using a single cell pressure probe to directly measure turgor in Vaucheria after hyperosmotic shock, V. erythrospora was found to recover turgor after a larger shock than V. repens. Threshold shock values for this ability were >0.5 MPa for V. erythrospora and <0.5 MPa for V. repens. Recovery was more rapid in V. erythrospora than V. repens after comparable shocks. Turgor recovery in V. erythrospora was inhibited by Gd³⁺ and TEA, suggesting a role for mechanosensitive channels, nonselective cation channels, and K⁺ channels in the process. Growth studies showed that V. erythrospora was able to grow over a wider range of NaCl concentrations. These responses may underlie the ability of V. erythrospora to survive in an estuarine habitat and restrict V. repens to freshwater. The fact that both species can turgor regulate may indicate a fundamental difference between members of the Stramenopila, as research to date on oomycetes suggests they are unable to turgor regulate.     

Comparative characterization of novel ene‐reductases from cyanobacteria

The growing importance of biocatalysis in the syntheses of enantiopure molecules results from the benefits of enzymes regarding selectivity and specificity of the reaction and ecological issues of the process. Ene‐reductases (ERs) from the old yellow enzyme family have received much attention in the last years. These flavo‐enzymes catalyze the trans‐specific reduction of activated C?C bonds, which is an important reaction in asymmetric synthesis, because up to two stereogenic centers can be created in one reaction. However, limitations of ERs described in the literature such as their moderate catalytic activity and their strong preference for NADPH promote the search for novel ERs with improved properties. In this study, we characterized nine novel ERs from cyanobacterial strains belonging to different taxonomic orders and habitats. ERs were identified with activities towards a broad spectrum of alkenes. The reduction of maleimide was catalyzed with activities of up to 35.5 U mg^?1 using NADPH. Ketoisophorone and (R)‐carvone, which were converted to the highly valuable compounds (R)‐levodione and (2R,5R)‐dihydrocarvone, were reduced with reaction rates of up to 2.2 U mg^?1 with NADPH. In contrast to other homologous ERs from the literature, NADH was accepted at moderate to high rates as well: Enzyme activities of up to 16.7 U mg^?1 were obtained for maleimide and up to 1.3 U mg^?1 for ketoisophorone and (R)‐carvone. Additionally, excellent stereoselectivities were achieved in the reduction of (R)‐carvone (97–99% de). In particular, AnabaenaER3 from Anabaena variabilis ATCC 29413 and AcaryoER1 from Acaryochloris marina MBIC 11017 were identified as useful biocatalysts. Therefore, novel ERs from cyanobacteria with high catalytic efficiency were added to the toolbox for the asymmetric reduction of alkenes. Biotechnol. Bioeng. 2013; 110: 1293–1301. © 2012 Wiley Periodicals, Inc.     

Generation of the antioxidant yellow pigment derived from 4-methylthio-3-butenyl isothiocyanate in salted radish roots (takuan-zuke)

2-[3-(2-Thioxopyrrolidin-3-ylidene)methyl]-tryptophan (TPMT) is a yellow pigment of salted radish roots (takuan-zuke) derived from 4-methylthio-3-butenyl isothiocyanate (MTBITC), the pungent component of radish roots. Here, we prepared salted radish and analyzed the behavior of the yellow pigment and related substances in the dehydration process and long-term salting process. All salted radish samples turned yellow, and their b^* values increased with time and temperature. The salted radish that was sun-dried and pickled at room temperature turned the brightest yellow, and the generation of TPMT was clearly confirmed. These results indicate that tissue shrinkage due to dehydration, salting temperature, and pH play important roles in the yellowing of takuan-zuke.     

The autophagy pathway participates in resistance to tomato yellow leaf curl virus infection in whiteflies

Macroautophagy/autophagy plays an important role against pathogen infection in mammals and plants. However, little has been known about the role of autophagy in the interactions of insect vectors with the plant viruses, which they transmit. Begomoviruses are a group of single-stranded DNA viruses and are exclusively transmitted by the whitefly Bemisia tabaci in a circulative manner. In this study, we found that the infection of a begomovirus, tomato yellow leaf curl virus (TYLCV) could activate the autophagy pathway in the Middle East Asia Minor 1 (MEAM1) species of the B. tabaci complex as evidenced by the formation of autophagosomes and ATG8-II. Interestingly, the activation of autophagy led to the subsequent degradation of TYLCV coat protein (CP) and genomic DNA. While feeding the whitefly with 2 autophagy inhibitors (3-methyladenine and bafilomycin A₁) and silencing the expression of Atg3 and Atg9 increased the viral load; autophagy activation via feeding of rapamycin notably decreased the amount of viral CP and DNA in the whitefly. Furthermore, we found that activation of whitefly autophagy could inhibit the efficiency of virus transmission; whereas inhibiting autophagy facilitated virus transmission. Taken together, these results indicate that TYLCV infection can activate the whitefly autophagy pathway, which leads to the subsequent degradation of virus. Furthermore, our report proves that an insect vector uses autophagy as an intrinsic antiviral program to repress the infection of a circulative-transmitted plant virus. Our data also demonstrate that TYLCV may replicate and trigger complex interactions with the insect vector.