全文获取类型
收费全文 | 945篇 |
免费 | 24篇 |
国内免费 | 28篇 |
专业分类
997篇 |
出版年
2023年 | 5篇 |
2022年 | 11篇 |
2021年 | 8篇 |
2020年 | 8篇 |
2019年 | 17篇 |
2018年 | 23篇 |
2017年 | 18篇 |
2016年 | 9篇 |
2015年 | 16篇 |
2014年 | 32篇 |
2013年 | 97篇 |
2012年 | 21篇 |
2011年 | 42篇 |
2010年 | 29篇 |
2009年 | 45篇 |
2008年 | 46篇 |
2007年 | 36篇 |
2006年 | 36篇 |
2005年 | 51篇 |
2004年 | 33篇 |
2003年 | 32篇 |
2002年 | 36篇 |
2001年 | 27篇 |
2000年 | 16篇 |
1999年 | 20篇 |
1998年 | 13篇 |
1997年 | 19篇 |
1996年 | 15篇 |
1995年 | 22篇 |
1994年 | 16篇 |
1993年 | 28篇 |
1992年 | 13篇 |
1991年 | 10篇 |
1990年 | 11篇 |
1989年 | 14篇 |
1988年 | 19篇 |
1987年 | 11篇 |
1986年 | 13篇 |
1985年 | 9篇 |
1984年 | 15篇 |
1983年 | 9篇 |
1982年 | 9篇 |
1981年 | 5篇 |
1980年 | 8篇 |
1978年 | 6篇 |
1977年 | 4篇 |
1975年 | 3篇 |
1974年 | 2篇 |
1972年 | 2篇 |
1971年 | 2篇 |
排序方式: 共有997条查询结果,搜索用时 15 毫秒
961.
Karl A.P. Payne 《FEBS letters》2010,584(6):1231-1234
Like many other aerobic archaea, the hyperthermophile Sulfolobus solfataricus possesses a gene cluster encoding components of a putative 2-oxoacid dehydrogenase complex. In the current paper, we have cloned and expressed the first two genes of this cluster and demonstrate that the protein products form an α2β2 hetero-tetramer possessing the catalytic activity characteristic of the first component enzyme of an acetoin dehydrogenase multienzyme complex. This represents the first report of an acetoin multienzyme complex in archaea, and contrasts with the branched-chain 2-oxoacid dehydrogenase complex activities characterised in two other archaea, Thermoplasma acidophilum and Haloferax volcanii. 相似文献
962.
Nonneuronal Localization for Steroid Converting Enzyme: 3α-Hydroxysteroid Oxidoreductase in Olfactory Tubercle of Rat Brain 总被引:1,自引:1,他引:0
3 alpha-Hydroxysteroid oxidoreductase (EC 1.1.1.50) was localized in the rat brain by cryostat sectioning, microassay, and neurochemical lesions. Single 16-microns sections were cut, homogenized, and assayed. In the olfactory tubercle 3 alpha-hydroxysteroid oxidoreductase activity is high in the piaglial layer at the surface, 20-fold lower at a depth of 50 microns, and 50-fold lower at a depth of 200 microns. A similar pattern of activity was seen in the olfactory bulb, the interpeduncular nucleus, the frontal pole of the cortex, and the frontoparietal cortex. When kainic acid, a toxin that destroys neurons but leaves glia and axons of passage intact, was injected into the olfactory tubercle, 3 alpha-hydroxysteroid oxidoreductase activity was undiminished whereas glutamic acid decarboxylase activity was reduced by 80%. This laminar distribution and insensitivity to kainic acid are consistent with a nonneuronal localization. The high concentration of astrocytes in the piaglial layer, where 3 alpha-hydroxysteroid oxidoreductase activity is highest, lead us to suggest that this enzyme is localized to astrocytes. The presence of particular enzymes in some brain regions and not in others determines which products are synthesized and which are inactivated in those regions. Thus, the location of 3 alpha-hydroxysteroid oxidoreductase and other steroid converting enzymes can affect the activity of neuronal circuits and the behaviours regulated by those circuits. 相似文献
963.
András Tóth Mária Takács Géza Groma Gábor Rákhely & Kornél L. Kovács 《FEMS microbiology letters》2008,282(1):8-14
Thermococcus litoralis , a hyperthermophilic Archaeon, is able to reduce elemental sulfur during fermentative growth. An unusual gene cluster ( nsoABCD ) was identified in this organism. In silico analysis suggested that three of the genes ( nsoABC ) probably originated from Eubacteria and one gene ( nsoD ) from Archaea. The putative NsoA and NsoB are similar to NuoE- and NuoF-type electron transfer proteins, respectively. NsoC has a unique domain structure and contains a GltD domain, characteristic of glutamate synthase small subunits, which seems to be integrated into a NuoG-type sequence. Flavin and NAD(P)H binding sites and conserved cysteines forming iron–sulfur clusters binding motifs were identified in the protein sequences deduced. The purified recombinant NsoC contains one FAD cofactor per protein molecule and catalyzes the reduction of polysulfide with NADPH as an electron donor and it also reduces oxygen. It was concluded that the Nso complex is a new type of NADPH-oxidizing enzyme using sulfur and/or oxygen as an electron acceptor. 相似文献
964.
It was reported that VDAC1 possesses an NADH oxidoreductase activity and plays an important role in the activation of xenobiotics in the outer mitochondrial membrane. In the present work, we evaluated the participation of VDAC1 and Cyb5R3 in the NADH-dependent activation of various redox cyclers in mitochondria. We show that external NADH oxidoreductase caused the redox cycling of menadione ≫ lucigenin>nitrofurantoin. Paraquat was predominantly activated by internal mitochondria oxidoreductases. An increase in the ionic strength stimulated and suppressed the redox cycling of negatively and positively charged acceptors, as was expected for the Cyb5R3-mediated reduction. Antibodies against Cyb5R3 but not VDAC substantially inhibited the NADH-related oxidoreductase activities. The specific VDAC blockers G3139 and erastin, separately or in combination, in concentrations sufficient for the inhibition of substrate transport, exhibited minimal effects on the redox cycler-dependent NADH oxidation, ROS generation, and reduction of exogenous cytochrome c. In contrast, Cyb5R3 inhibitors (6-propyl-2-thiouracil, p-chloromercuriobenzoate, quercetin, mersalyl, and ebselen) showed similar patterns of inhibition of ROS generation and cytochrome c reduction. The analysis of the spectra of the endogenous cytochromes b5 and c in the presence of nitrofurantoin and the inhibitors of VDAC and Cyb5R3 demonstrated that the redox cycler can transfer electrons from Cyb5R3 to endogenous cytochrome c. This caused the oxidation of outer membrane-bound cytochrome b5, which is in redox balance with Cyb5R3. The data obtained argue against VDAC1 and in favor of Cyb5R3 involvement in the activation of redox cyclers in the outer mitochondrial membrane. 相似文献
965.
Egbert J. Boekema Jan F.L. Van Breemen Wilko Keegstra Ernst F.J. Van Bruggen Simon P.J. Albracht 《BBA》1982,679(1):7-11
Two-dimensional crystalline arrays of NADH:Q oxidoreductase preparations have been obtained by microdiffusion of protein dissolved in detergent against a 15 mM sodium acetate buffer of pH 5.5 containing 10% () ammonium sulphate. Electron microscopy was used to study the structure of negatively stained crystals. Computer-reconstructed images were obtained by the Fourier peak filtering method. The crystals have p4 symmetry and a square unit cell with dimensions of 15.2 ± 0.5 nm. The four asymmetric units in the unit cell form a single tetrameric molecule with a dimension in the third direction of 8.2 nm. It is concluded on the basis of the estimated molecular mass that each tetramer cannot contain more than only one FMN molecule. This implies that the tetramers possibly are only a part of Complex I, since there is much evidence that one functional enzyme molecule of Complex I contains two FMN molecules. 相似文献
966.
The membrane system which epoxidizes aldrin to dieldrin in pea roots was separated from polyphenolic inhibitors and soluble peroxidase by rapid anaerobic gel filtration. Epoxidase activity was strongly inhibited by cytochrome-c, cyanide, electron acceptors and polyphenols and was enhanced by chelating and solubilizing agents which inhibit cytochrome P450 systems. 相似文献
967.
《Journal of enzyme inhibition and medicinal chemistry》2013,28(2):267-274
Claisen-Schmidt condensation of 3-(1,2,3,6-tetrahydro-1-methylpyridin-4-yl)-2,4,5- trimethoxybenzaldehyde 3 and various aromatic, heterocyclic and alicyclic amides of 3- aminoacetophenone 6(a–s) afforded novel curcumin mimics. All the synthesized compounds were characterized by IR, 1H NMR, Mass spectroscopy and evaluated for antioxidant, cytotoxicity and antimicrobial activity. Out of the 20 compounds screened, compounds 7i, 7l, 7q, and 7n have shown excellent radical scavenging activity, compounds 7o, 7t, 7f, and 7r have shown significant xanthine oxidase inhibition, and compounds 7a, 7k and 7l were found to be potent inhibitors of selected cancer cell lines. Compounds 7h, 7t, 7l, 7i, and 7e have shown good antibacterial activity, whereas compounds 7j, 7f, 7o, 7h, and 7t exhibited significant antifungal activity. 相似文献
968.
Maria S. Fadeeva Cinthia Núñez Yulia V. Bertsova Guadalupe Espín & Alexander V. Bogachev 《FEMS microbiology letters》2008,279(1):116-123
The catalytic properties of sodium-translocating NADH:quinone oxidoreductases (Na+ -NQRs) from the marine bacterium Vibrio harveyi , the enterobacterium Klebsiella pneumoniae , and the soil microorganism Azotobacter vinelandii have been comparatively analyzed. It is shown that these enzymes drastically differ in their affinity to sodium ions. The enzymes also possess different sensitivity to inhibitors. Na+ -NQR from A. vinelandii is not sensitive to low 2- n -heptyl-4-hydroxyquinoline N-oxide (HQNO) concentrations, while Na+ -NQR from K. pneumoniae is fully resistant to either Ag+ or N-ethylmaleimide. All the Na+ -NQR-type enzymes are sensitive to diphenyliodonium, which is shown to modify the noncovalently bound FAD of the enzyme. 相似文献
969.
Annamária Kósa 《BBA》2006,1757(7):811-820
Artificial formation of flash-photoactive oligomeric protochlorophyllide complexes was found in etiolated pea (Pisum sativum L. cv. Zsuzsi) epicotyl homogenates containing glycerol (40% v/v) and sucrose (40% m/v). The 77 K fluorescence emission spectra indicated that the ratio of the 644 and 655 nm emitting forms to the 636 nm form increased during 3 to 5-day incubation in the dark at −14 °C. Electron micrographs showed the presence of well-organized prolamellar bodies in the homogenates. The same phenomena were found when the homogenates were frozen into liquid nitrogen and thawed to room temperature in several cycles. Similar treatments of intact epicotyl pieces caused significant membrane destructions. In homogenates, the in vitro produced 644 and 655 nm emitting protochlorophyllide forms were flash-photoactive; the extent of phototransformation increased compared to that in native epicotyls. The newly appeared 692 nm chlorophyllide band showed a blue shift (similar to the Shibata shift in leaves), however this process took place only partially due to the effect of the isolation medium.These results prove that the in vitro accumulated 644 and 655 nm protochlorophyllide forms were produced from the flash-photoactive 636 nm emitting monomeric NADPH:protochlorophyllide oxidoreductase units via aggregation, in connection with structure stabilization properties of glycerol and sucrose. 相似文献
970.
《Journal of enzyme inhibition and medicinal chemistry》2013,28(6):1332-1335
Barlerisides A (1) and B (2), new phenolic glycosides, have been isolated from the n-butanol soluble sub-fraction of Barleria acanthoides along with two known compounds acteoside (3) and p-hydroxycinnamic acid (4). Their structures have been assigned on the basis of spectral studies. Both 1 and 2 showed significant activity in the superoxide scavenging assay while weak inhibitory activity was observed against the enzyme xanthine oxidase. 相似文献