全文获取类型
收费全文 | 11173篇 |
免费 | 826篇 |
国内免费 | 802篇 |
出版年
2023年 | 175篇 |
2022年 | 223篇 |
2021年 | 308篇 |
2020年 | 320篇 |
2019年 | 405篇 |
2018年 | 403篇 |
2017年 | 353篇 |
2016年 | 284篇 |
2015年 | 377篇 |
2014年 | 540篇 |
2013年 | 698篇 |
2012年 | 371篇 |
2011年 | 436篇 |
2010年 | 370篇 |
2009年 | 450篇 |
2008年 | 506篇 |
2007年 | 550篇 |
2006年 | 525篇 |
2005年 | 430篇 |
2004年 | 375篇 |
2003年 | 382篇 |
2002年 | 391篇 |
2001年 | 273篇 |
2000年 | 274篇 |
1999年 | 202篇 |
1998年 | 213篇 |
1997年 | 201篇 |
1996年 | 201篇 |
1995年 | 214篇 |
1994年 | 215篇 |
1993年 | 189篇 |
1992年 | 190篇 |
1991年 | 167篇 |
1990年 | 155篇 |
1989年 | 151篇 |
1988年 | 159篇 |
1987年 | 142篇 |
1986年 | 98篇 |
1985年 | 149篇 |
1984年 | 133篇 |
1983年 | 81篇 |
1982年 | 94篇 |
1981年 | 102篇 |
1980年 | 62篇 |
1979年 | 53篇 |
1978年 | 41篇 |
1977年 | 34篇 |
1976年 | 41篇 |
1973年 | 26篇 |
1972年 | 20篇 |
排序方式: 共有10000条查询结果,搜索用时 750 毫秒
991.
D. Frame 《Plant biology (Stuttgart, Germany)》2003,5(3):290-296
Abstract: The pathway of the pollen tube in Tasmannia insipida (Winteraceae), an archaic angiosperm, is described. Visual observations and measurements of percentage sugar content from stigmatic exudate and the calyptra droplet were made. The droplet that forms on the calyptra of unopened flowers was artificially pollinated, but no pollen tubes appeared to grow through it to the gynoecium of female flowers. Calyptra droplets occur on both female and male (having reduced female structures) flowers of this dioecious plant. It is considered unlikely that a drop pollination mechanism, analogous to that found in some gymnosperms, is at work in T. insipida, and it is proposed that one possible droplet function is as a "reward" to potential pollinators in advance of flower opening. Pollen tubes were observed to grow almost exclusively along epidermal cells. Early thoughts on angiosperm transmission tissue are re-examined and built upon. It is proposed that the male gametophyte conduction tissue in angiosperms is homologous with epidermal tissue, and that pollen tube passage occurred originally solely along the surface of specialized epidermal cells, or possibly for short distances along unspecialized regions covered with exudate derived from these cells. Some earlier attempts to explain transmitting tissue homology and evolution are discussed. 相似文献
992.
The numerical solution of the coupled system of partial differential and ordinary differential equations that model the whole
heart in three dimensions is a considerable computational challenge. As a consequence, it is not computationally practical—either
in terms of memory or time—to repeat simulations on a finer computational mesh to ensure that convergence of the solution
has been attained. In an attempt to avoid this problem while retaining mathematical rigour, we derive a one dimensional model
of a cardiac fibre that takes account of elasticity properties in three structurally defined axes within the myocardial tissue.
This model of a cardiac fibre is then coupled with an electrophysiological cell model and a model of cellular electromechanics
to allow us to simulate the coupling of the electrical and mechanical activity of the heart. We demonstrate that currently
used numerical methods for coupling electrical and mechanical activity do not work in this case, and identify appropriate
numerical techniques that may be used when solving the governing equations. This allows us to perform a series of simulations
that: (i) investigate the effect of some of the assumptions inherent in other models; and (ii) reproduce qualitatively some
experimental observations. 相似文献
993.
Tingey DT Lee EH Phillips DL Rygiewicz PT Waschmann RS Johnson MG Olszyk DM 《Plant, cell & environment》2007,30(11):1400-1410
We investigated the effects of elevated CO(2) (EC) [ambient CO(2) (AC) + 190 ppm] and elevated temperature (ET) [ambient temperature (AT) + 3.6 degrees C] on net ecosystem exchange (NEE) of seedling Douglas fir (Pseudotsuga menziesii) mesocosms. As the study utilized seedlings in reconstructed soil-litter-plant systems, we anticipated greater C losses through ecosystem respiration (R(e)) than gains through gross photosynthesis (GPP), i.e. negative NEE. We hypothesized that: (1) EC would increase GPP more than R(e), resulting in NEE being less negative; and (2) ET would increase R(e) more than GPP, resulting in NEE being more negative. We also evaluated effects of CO(2) and temperature on light inhibition of dark respiration. Consistent with our hypothesis, NEE was a smaller C source in EC, not because EC increased photosynthesis but rather because of decreased respiration resulting in less C loss. Consistent with our hypothesis, NEE was more negative in ET because R(e) increased more than GPP. The light level that inhibited respiration varied seasonally with little difference among CO(2) and temperature treatments. In contrast, the degree of light inhibition of respiration was greater in AC than EC. In our system, respiration was the primary control on NEE, as EC and ET caused greater changes in respiration than photosynthesis. 相似文献
994.
Data on the concentration of the elements in the human body are important, for example, to estimate the amounts required to
maintain a good healthy state or find their connections with morbidity and mortality. In this paper, the concentration of
copper (by flame atomic absorption spectrometry) in material obtained from autopsy cases of nonpoisoned people (n = 130), aged from 14 to 80 years, between 1990–2006, is presented. The following values were found (mean ± SD in micrograms
of copper per gram or per milliliter): brain 3.32 ± 1.50 (n = 43), liver 3.47 ± 1.51 (n = 79), kidney 2.15 ± 0.90 (n = 76), stomach 1.10 ± 0.76 (n = 65), intestines 1.54 ± 1.19 (n = 25), lung 1.91 ± 1.30 (n = 27), spleen 1.23 ± 0.28 (n = 3), heart 3.26 ± 0.59 (n = 5), bile 3.60 ± 1.67 (n = 13), and blood 0.85 ± 0.19 (n = 73). 相似文献
995.
Methanomicrococcus blatticola is an obligately anaerobic methanogen that derives the energy for growth exclusively from the reduction of methylated compounds to methane with molecular hydrogen as energy source. Competition for methanol (concentration below 10 microM) and H(2) (concentration below 500 Pa), as well as oxidative stress due to the presence of oxygen are likely to occur in the peripheral region of the cockroach hindgut, the species' normal habitat. We investigated the ecophysiological properties of M. blatticola to explain how it can successfully compete for its methanogenic substrates. The organism showed affinities for methanol (K(m)=5 microM; threshold<1 microM) and hydrogen (K(m)=200 Pa; threshold <0.7 Pa) that are superior to other methylotrophic methanogens (Methanosphaera stadtmanae, Methanosarcina barkeri) investigated here. Thermodynamic considerations indicated that 'methanol respiration', i.e. the use of methanol as the terminal electron acceptor, represents an attractive mode of energy generation, especially at low hydrogen concentrations. Methanomicrococcus blatticola exploits the opportunities by specific growth rates (>0.2 h(-1)) and specific growth yields (up to 7 g of dry cells per mole of methane formed) that are particularly high within the realm of mesophilic methanogens. Upon oxygen exposure, part of the metabolic activity may be diverted into oxygen removal, thus establishing appropriate anaerobic conditions for survival and growth. 相似文献
996.
997.
Klaus S. Larsen Andreas Ibrom Claus Beier Sven Jonasson Anders Michelsen 《Biogeochemistry》2007,85(2):201-213
We measured net ecosystem CO2 flux (F
n) and ecosystem respiration (R
E), and estimated gross ecosystem photosynthesis (P
g) by difference, for two years in a temperate heath ecosystem using a chamber method. The exchange rates of carbon were high
and of similar magnitude as for productive forest ecosystems with a net ecosystem carbon gain during the second year of 293 ± 11 g C m−2 year−1 showing that the carbon sink strength of heather-dominated ecosystems may be considerable when C. vulgaris is in the building phase of its life cycle. The estimated gross ecosystem photosynthesis and ecosystem respiration from October
to March was 22% and 30% of annual flux, respectively, suggesting that both cold-season carbon gain and loss were important
in the annual carbon cycle of the ecosystem. Model fit of R
E of a classic, first-order exponential equation related to temperature (second year; R
2 = 0.65) was improved when the P
g rate was incorporated into the model (second year; R
2 = 0.79), suggesting that daytime R
E increased with increasing photosynthesis. Furthermore, the temperature sensitivity of R
E decreased from apparent Q
10 values of 3.3 to 3.9 by the classic equation to a more realistic Q
10 of 2.5 by the modified model. The model introduces R
photo, which describes the part of respiration being tightly coupled to the photosynthetic rate. It makes up 5% of the assimilated
carbon dioxide flux at 0°C and 35% at 20°C implying a high sensitivity of respiration to photosynthesis during summer. The
simple model provides an easily applied, non-intrusive tool for investigating seasonal trends in the relationship between
ecosystem carbon sequestration and respiration. 相似文献
998.
A comparison was made of the structures of the Fnr and ArcA modulons and regulons. The data on modulon composition were taken from published microarray assays, whereas regulons were characterized using comparative genomic approaches. The regulatory cascade involving Fnr and ArcA contributes greatly to the extension of the Fnr modulon over the Fnr regulon by adding operons of the ArcA modulon. The Fnr and ArcA regulons were shown to contain 26 and 16 operons, respectively. Ten operons had high-score and highly conserved sites for both Fnr and ArcA and were isolated as a so-called core of regulons. 相似文献
999.
Acclimation of photosynthesis and respiration is asynchronous in response to changes in temperature regardless of plant functional group 总被引:3,自引:1,他引:2
Campbell C Atkinson L Zaragoza-Castells J Lundmark M Atkin O Hurry V 《The New phytologist》2007,176(2):375-389
Gas exchange, fluorescence, western blot and chemical composition analyses were combined to assess if three functional groups (forbs, grasses and evergreen trees/shrubs) differed in acclimation of leaf respiration (R) and photosynthesis (A) to a range of growth temperatures (7, 14, 21 and 28 degrees C). When measured at a common temperature, acclimation was greater for R than for A and differed between leaves experiencing a 10-d change in growth temperature (PE) and leaves newly developed at each temperature (ND). As a result, the R : A ratio was temperature dependent, increasing in cold-acclimated plants. The balance was largely restored in ND leaves. Acclimation responses were similar among functional groups. Across the functional groups, cold acclimation was associated with increases in nonstructural carbohydrates and nitrogen. Cold acclimation of R was associated with an increase in abundance of alternative and/or cytochrome oxidases in a species-dependent manner. Cold acclimation of A was consistent with an initial decrease and subsequent recovery of thylakoid membrane proteins and increased abundance of proteins involved in the Calvin cycle. Overall, the results point to striking similarities in the extent and the biochemical underpinning of acclimation of R and A among contrasting functional groups differing in overall rates of metabolism, chemical composition and leaf structure. 相似文献
1000.