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41.
Vik SB 《Journal of bioenergetics and biomembranes》2000,32(5):485-491
The ATP synthase from Escherichia coli is a prototype of the ATP synthases that are found in many bacteria, in the mitochondria of eukaryotes, and in the chloroplasts of plants. It contains eight different types of subunits that have traditionally been divided into F1, a water-soluble catalytic sector, and Fo, a membrane-bound ion transporting sector. In the current rotary model for ATP synthesis, the subunits can be divided into rotor and stator subunits. Several lines of evidence indicate that is one of the three rotor subunits, which rotate through 360 degrees. The three-dimensional structure of is known and its interactions with other subunits have been explored by several approaches. In light of recent work by our group and that of others, the role of in the ATP synthase from E. coli is discussed. 相似文献
42.
Colonization at sugar beet root surfaces by seedling-inoculated biocontrol strain Pseudomonas fluorescens DR54 and native soil bacteria was followed over a period of 3 weeks using a combination of immunofluorescence (DR54-targeting specific antibody) and fluorescence in situ hybridization (rRNA-targeting Eubacteria EUB338 probe) techniques with confocal laser scanning microscopy. The dual staining protocol allowed cellular activity (ribosomal number) to be recorded in both single cells and microcolonies of strain DR54 during establishment on the root. After 2 days, the population density of strain DR54 reached a constant level at the root basis. From this time, however, high cellular activity was only found in few bacteria located as single cells, whereas all microcolony-forming cells occurring in aggregates were still active. In contrast, a low density of strain DR54 was observed at the root tip, but here many of the bacteria located as single cells were active. The native population of soil bacteria, comprising a diverse assembly of morphologically different forms and size classes, initiated colonization at the root basis only after 2 days of incubation. Hence the dual staining protocol allowed direct microscopic studies of early root colonization by both inoculant and native soil bacteria, including their differentiation into active and non-active cells and into single or microcolony-forming cells. 相似文献
43.
The aim of this study was to determine if endophytic bacteria could contribute to cultivar specific interactions between red clover (Trifolium pratense L.) and potatoes (Solanum tuberosum L.) in crop rotations. Endophytic bacteria were isolated from the roots of four red clover cultivars (AC Charlie, Altaswede, Marino and Tempus) grown in the field. Populations of bacteria from each cultivar were similar. The most abundant genus was Rhizobium, but species of Curtobacterium, Pseudomonas, and Xanthomonas were common to all cultivars. Plantlets of two potato cultivars, Russet Burbank and Shepody, were inoculated individually with the seven bacterial isolates most frequently recovered from each red clover cultivar, and grown in Magenta vessels for 6 wk. Significant differences were found for plant height, and wet weights of roots, shoots and their total. Potato cultivars differed for root wet weight only, while red clover cultivar, as a source of bacteria, had a significant effect on all traits except plant height. Differences among bacteria were significant for all traits except shoot wet weight. There was a significant interaction of potato cultivar by red clover cultivar. The potato cultivar Russet Burbank did best with bacteria from the red clover cultivar, Marino; and Shepody, with bacteria from Altaswede. 相似文献
44.
Early phosphorus nutrition,mycorrhizae development,dry matter partitioning and yield of maize 总被引:7,自引:1,他引:6
We conducted a field experiment to test the hypothesis that improved phosphorus nutrition occurs in maize plants with rapid arbuscular (AM) mycorrhizae development at early developmental stages and that this also is reflected in dry matter allocation and final yield. A split-split plot design was used with previous crop (Zea mays L.-maize and Brassica napus L.-canola), tillage practices (no-tillage or conventional tillage) and P fertilization (5 levels) as factors chosen to modify mycorrhizae development at early developmental stages of maize. Previous cropping with canola resulted in decreased shoot-P concentration and shoot growth of maize at early stages. No-tillage resulted in higher shoot-P concentration but lower shoot weight than conventional tillage. Greater shoot-P uptake was related to a rapid intraradical development of mycorrhizae (previous crop of maize) or rapid connection to a mycorrhizal mycelium network (no-tillage treatments). Maize yield and harvest index were lower after cropping with canola. The yield for conventional tillage was higher than that for no-tillage but the harvest index was lower. The hypothesis was supported at early stages of maize growth by the effect of previous crop but not by results of tillage, because an unknown factor reduced growth in the no-tillage system. The hypothesis was supported at maturity by increased biomass allocation to grain relative to total shoot weight in treatments with greater shoot-P concentration at early stages. 相似文献
45.
In vivo baseline measurements of hip joint range of motion in suspensory and nonsuspensory anthropoids
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Ashley S. Hammond 《American journal of physical anthropology》2014,153(3):417-434
Hominoids and atelines are known to use suspensory behaviors and are assumed to possess greater hip joint mobility than nonsuspensory monkeys, particularly for range of abduction. This assumption has greatly influenced how extant and fossil primate hip joint morphology has been interpreted, despite the fact that there are no data available on hip mobility in hominoids or Ateles. This study uses in vivo measurements to test the hypothesis that suspensory anthropoids have significantly greater ranges of hip joint mobility than nonsuspensory anthropoids. Passive hip joint mobility was measured on a large sample of anesthetized captive anthropoids (nonhuman hominids = 43, hylobatids = 6, cercopithecids = 43, Ateles = 6, and Cebus = 6). Angular and linear data were collected using goniometers and tape measures. Range of motion (ROM) data were analyzed for significant differences by locomotor group using ANOVA and phylogenetic regression. The data demonstrate that suspensory anthropoids are capable of significantly greater hip abduction and external rotation. Degree of flexion and internal rotation were not larger in the suspensory primates, indicating that suspension is not associated with a global increase in hip mobility. Future work should consider the role of external rotation in abduction ability, how the physical position of the distal limb segments are influenced by differences in ROM proximally, as well as focus on bony and soft tissue differences that enable or restrict abduction and external rotation at the anthropoid hip joint. Am J Phys Anthropol 153:417–434, 2014. © 2013 Wiley Periodicals, Inc. 相似文献
46.
Western scrub-jays (Aphelocoma californica) hide food and rely on spatial memory to recover their caches at a later date. They also rely on observational spatial memory
to steal caches made by other individuals. Successful pilfering may require an understanding of allocentric space because
the observer will often be in a different position from the demonstrator when the caching event occurs. We compared cache
recovery accuracy of pairs of observers that watched a demonstrator cache food. The pattern of recovery searches showed that
observers were more accurate when they had observed the caching event from the same viewing direction as the demonstrator
than when they had watched from the opposite direction. Search accuracy was not affected by whether or not the tray-specific
local cues provided left–right landmark information (i.e. heterogeneous vs. homogeneous local cues), or whether or not the
caching tray location was rotated. Taken together, these results suggest that observers have excellent spatial memory and
that they have little difficulty with mental rotation. 相似文献
47.
A chiral column, with decaproline as the chiral selector, has broad chiral selectivity. To understand the separation mechanism of this chiral column, multiple spectroscopic techniques, including optical rotation, electronic circular dichroism, infrared absorption and vibrational circular dichroism, have been used here to study the conformation of the decaproline oligomer in isopropanol(IPA)/dichloromethane(DCM) mixtures. These studies indicate that decaproline oligomer adopts polyproline II conformation in IPA/DCM solvent system (0% IPA approximately 100% IPA). Hydrogen bonding interactions between C=O groups of decaproline and IPA molecules increase as the content of IPA in the solvent mixture increases up to 60% and become less significant from then onwards. These spectroscopic observations are found to have a good correlation with the enantiomeric separation of racemic 2,2,2-trifluoro-1-[10-(2,2,2-trifluoro-1-hydroxy-ethyl-anthracen-9-yl]-ethanol by the decaproline column. 相似文献
48.
Danny A. P. Hooftman James M. Bullock Kathryn Morley Caroline Lamb David J. Hodgson Philippa Bell Jane Thomas Rosemary S. Hails 《Annals of botany》2015,115(1):147-157
Background and Aims Gene flow from crops to their wild relatives has the potential to alter population growth rates and demography of hybrid populations, especially when a new crop has been genetically modified (GM). This study introduces a comprehensive approach to assess this potential for altered population fitness, and uses a combination of demographic data in two habitat types and mathematical (matrix) models that include crop rotations and outcrossing between parental species.Methods Full life-cycle demographic rates, including seed bank survival, of non-GM Brassica rapa × B. napus F1 hybrids and their parent species were estimated from experiments in both agricultural and semi-natural habitats. Altered fitness potential was modelled using periodic matrices including crop rotations and outcrossing between parent species.Key Results The demographic vital rates (i.e. for major stage transitions) of the hybrid population were intermediate between or lower than both parental species. The population growth rate (λ) of hybrids indicated decreases in both habitat types, and in a semi-natural habitat hybrids became extinct at two sites. Elasticity analyses indicated that seed bank survival was the greatest contributor to λ. In agricultural habitats, hybrid populations were projected to decline, but with persistence times up to 20 years. The seed bank survival rate was the main driver determining persistence. It was found that λ of the hybrids was largely determined by parental seed bank survival and subsequent replenishment of the hybrid population through outcrossing of B. rapa with B. napus.Conclusions Hybrid persistence was found to be highly dependent on the seed bank, suggesting that targeting hybrid seed survival could be an important management option in controlling hybrid persistence. For local risk mitigation, an increased focus on the wild parent is suggested. Management actions, such as control of B. rapa, could indirectly reduce hybrid populations by blocking hybrid replenishment. 相似文献
49.
黄土高原半干旱偏旱区草粮轮作田土壤水分恢复效应模拟 总被引:1,自引:0,他引:1
采用实地调查和EPIC模型相结合的方法,模拟研究了黄土高原半干旱偏旱区不同草粮轮作模式的土壤水分恢复效应.结果表明:多年生苜蓿地和苜蓿翻耕后种植粮食作物农田0~10 m土层土壤湿度调查值与模拟值间的相关系数均超过0.9(P<0.01),相对均方根误差在0.05~0.16,相对误差均低于10%;模拟与实测的土壤湿度剖面分布变化趋势一致.在黄土高原半干旱偏旱区,苜蓿地深层土壤干层恢复难度较大,在种植苜蓿期间应避免8~10 m土层土壤湿度降到5.7%以下.苜蓿地适宜种植年限为4~6 a,最长不应超过8 a.苜蓿翻耕后适宜采用马铃薯→马铃薯→春小麦轮作模式进行土壤水分恢复,32~33 a后可以再次种植苜蓿. 相似文献
50.
SAM RIFFELL JAKE VERSCHUYL DARREN MILLER T. BENTLY WIGLEY 《Global Change Biology Bioenergy》2011,3(4):313-321
Short‐rotation woody cropping (SRWC) refers to silvicultural systems designed to produce woody biomass using short harvest cycles (1–15 years), intensive silvicultural techniques, high‐yielding varieties, and often coppice regeneration. Recent emphasis on alternatives to fossil fuels has spurred interest in producing SRWC on privately owned and intensively managed forests of North America. We examined potential bird and small mammal response at the stand level to conversion of existing, intensively managed forests to SRWCs using meta‐analysis of existing studies. We found 257 effect sizes for birds (243 effect sizes) and mammals (14 effect sizes) from 8 studies involving Populus spp. plantations. Diversity and abundance of bird guilds were lower on short‐rotation plantations compared with reference woodlands, while abundance of individual bird species was more variable and not consistently higher or lower on SRWC plantations. Shrub‐associated birds were more abundant on SRWC plantations, but forest‐associated and cavity‐nesting birds were less abundant. Effects on birds appeared to decrease with age of the SRWC plantation, but plantation age was also confounded with variation in the type of reference forest used for comparison. Both guilds and species of mammals were less abundant on SRWC plantations. These conclusions are tentative because none of these studies directly compared SRWC plantations to intensively managed forests. Plantations of SRWCs could contribute to overall landscape diversity in forest‐dominated landscapes by providing shrubby habitat structure for nonforest species. However, extensive conversion of mature or intensively managed forests to SRWC would likely decrease overall diversity, especially if they replace habitat types of high conservation value. 相似文献