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71.
Summary It has previously been shown by Macey and Farmer (Biochim. Biophys. Acta
211:104–106, 1970) that phloretin inhibits urea transport across the human red cell membrane yet has no effect on water transport. Jennings and Solomon (J. Gen. Physiol.
67:381–397, 1976) have shown that there are separate lipid and protein binding sites for phloretin on the red cell membrane. We have now found that urea transport is inhibited by phloretin binding to the lipids with aK
1 of 25±8 m in reason-able agreement with theK
D
of 54±5 m for lipid binding. These experiments show that lipid/protein interactions can alter the conformational state of the urea transport protein. Phloretin binding to the protein site also modulates red cell urea transport, but the modulation is opposed by the specific stilbene anion transport inhibitor, DIDS (4,4-diisothiocyano-2,2-stilbene disulfonate), suggesting a linkage between the urea transport protein and band 3. Neither the lipid nor the protein phloretin binding site has any significant effect on water transport. Water transport is, however, inhibited by up to 30% in a pH-dependent manner by DIDS binding, which suggests that the DIDS/band 3 complex can modulate water transport. 相似文献
72.
The distribution of dens and den use by the red fox was studied in Kumamoto Prefecture between 1968 and 1982. A total of 80
dens were classified into natal, residential, and temporarily-visited dens. Natal dens comprised 12.4% of all dens. Relative
proportions were quite stable for a long period. This indicates a constant number of reproducing females in the area for a
considerable length of time. Seasonally the utilization of den showed three peaks in February, May and October. The peaks
coincided well with the peaks of reproductive activity of female foxes. The results of multiple regression analysis of distribution
of dens with respect to selected environmental parameters around densites indicated that the fox selectively utilize open
land, rather than densely vegetated areas. The persistence of dens, the selective utilization by foxes of open land and the
sizes of dens were all related to the breeding activities of female foxes inhabiting the area. Reproductive females usually
used plural dens (“den group”) in one reproductive season. The numbers of those females occuring in the area were maintained
at a steady level for fairy long periods. These indicate that the existence of a stable social relationship between females
mediated through occupancy of “den groups.” 相似文献
73.
Summary Fathead minnows, Pimephales promelas, and yellow perch, Perca flavescens, were transferred from moderately soft Lake Superior water (hardness 45mg/l as CaCO3) to very soft diluted Lake Superior water (hardness 4.5mg/l). Sulfuric acid was added in some treatments by means of a multichannel diluter. In very soft water, chloride cells proliferated in the gills, especially in the epithelium of the secondary lamellae. When exposed to acid, chloride cells were damaged and less abundant in the secondary lamellae, and blood osmolality was reduced at pH 5.0 (x = 188 mOsm/kg, 9 days exposure; normal 280 mOsm/kg) for the minnows and pH 4.1 (x = 218 mOsm/kg, 58 days exposure; normal 329 mOsm/kg) for the perch. Certain chloride cells which form gland-like clusters in the primary lamellae of perch gills showed little damage even at pH 4.1. The present study supports the view that chloride cells proliferate in very soft fresh water to help maintain ionic balances, and that damage to these cells in acidified soft water may be related to diminished ionoregulatory capacity. The greater acid tolerance of chloride cells of, and the higher blood osmolality maintained by, perch could help to explain the greater tolerance of this species to low pH. In some cases, a species' ability to acclimate to very soft water and acidified soft water may depend upon the number, distribution, and physiology of its chloride cells. 相似文献
74.
75.
DAVID W. FOUNTAIN F.L.S. JACQUELINE M. HOLDSWORTH HEATHER A. OUTRED F.L.S. 《Botanical journal of the Linnean Society. Linnean Society of London》1989,99(3):197-207
FOUNTAIN, D. W., HOLDSWORTH, J. M. & OUTRED, H. A., 1989. The dispersal unit of Dacrycarpus dacrydioides (A. Rich.) de Laubenfels (Podocarpaceae) and the significance of the fleshy receptacle. Dacrycarpus dacrydioides (formerly Podocarpus dacrydioides ) is an arborescent gymnosperm endemic to New Zealand. The high water content (43%) and sensitivity of viability towards desiccation, suggest the seeds are of the 'recalcitrant' type. The 'fruits' comprising a seed borne on a fleshy receptacle arc shed in large numbers. The development of the seed precedes the full development of succulence in the receptacle and at maturity the seed has a high relative water content (RWC) relative to the receptacle. Within the maturing reproductive unit, the receptacle buffers the seed against the effects of water stress. After shedding, seeds are rapidly desiccated in moving air, and viability is impaired below approximately 80"' RWC and abolished at 34", seed RWC. The presence of the receptacle during drying confers resistance to desiccation-associated damage. Five phases of desiccation sensitivity are distinguished in recognition of the protective role of the receptacle. It is suggested that the advantages associated with prolonging seed viability may have contributed to the evolutionary development of succulence in the reproductive unit. This might be considered as a selection pressure in a manner similar to the proposal that such fleshy structures are associated with seed dispersal by birds. 相似文献
76.
77.
Changes in gas exchange characteristics and water use efficiency of mangroves in response to salinity and vapour pressure deficit 总被引:12,自引:0,他引:12
Summary Measurements were made of the photosynthetic gas exchange properties and water use efficiency of 19 species of mangrove in 9 estuaries with different salinity and climatic regimes in north eastern Australia and Papua New Guinea. Stomatal conductance and CO2 assimilation rates differed significantly between species at the same locality, with the salt-secreting species, Avicennia marina, consistently having the highest CO2 assimilation rates and stomatal conductances. Proportional changes in stomatal conductance and CO2 assimilation rate resulted in constant and similar intercellular CO2 concentrations for leaves exposed to photon flux densities above 800 mol·m-2·s-1 in all species at a particular locality. In consequence, all species at the same locality had similar water use efficiencies. There were, however, significant differences in gas exchange properties between different localities. Stomatal conductance and CO2 assimilation rate both decreased with increasing salinity and with increasing leaf to air vapour pressure deficit (VPD). Furthermore, the slope of the relationship between assimilation rate and stomatal conductance increased, while intercellular CO2 concentration decreased, with increasing salinity and with decreasing ambient relative humidity. It is concluded from these results that the water use efficiency of mangroves increases with increasing environmental stress, in this case aridity, thereby maximising photosynthetic carbon fixation while minimising water loss.Contribution No. 459 from the Australian Institute of Marine Science 相似文献
78.
Summary In the Chihuahuan Desert of southern New Mexico, both water and nitrogen limit the primary productivity of Larrea tridentata, a xerophytic evergreen shrub. Net photosynthesis was positively correlated to leaf N, but only in plants that received supplemental water. Nutrient-use efficiency, defined as photosynthetic carbon gain per unit N invested in leaf tissue, declined with increasing leaf N. However, water-use efficiency, defined as the ratio of photosynthesis to transpiration, increased with increasing leaf N, and thus these two measures of resource-use efficiency were inversely correlated. Resorption efficiency was not significantly altered over the nutrient gradient, nor was it affected by irrigation treatments. Leaf longevity decreased significantly with fertilization although the absolute magnitude of this decrease was fairly small, in part due to a large background of insect-induced mortality. Age-specific gas exchange measurements support the hypothesis that leaf aging represents a redistribution of resources, rather than actual deterioration or declining resource-use efficiency. 相似文献
79.
Water use efficiency and carbon isotope composition of plants in a cold desert environment 总被引:9,自引:0,他引:9
Summary The effects of the availabilities of water and nitrogen on water use efficiency (WUE) of plants were investigated in a sagebrush steppe. The four species studied wereArtemisia tridentata (shrub),Ceratoides lanata (suffrutescent shrub),Elymus lanceolatus (rhizomatous grass), andElymus elymoides (tussock grass). Water and nitrogen levels were manipulated in a two-by-two factorial design resulting in four treatments: control (no additions), added water, added nitrogen, and added water and nitrogen. One instantaneous and two long-term indicators of WUE were used to testa priori predictions of the ranking of WUE among treatments. The short-term indicator was the instantaneous ratio of assimilation to transpiration (A/E). The long-term measures were 1) the slope of the relationship between conductance to water vapor and maximum assimilation and 2) the carbon isotope composition (13C) of plant material. Additional water decreased WUE, whereas additional nitrogen increased WUE. For both A/E and 13C, the mean for added nitrogen alone was significantly greater than the mean for added water alone, and means for the control and added water and nitrogen fell in between. This ranking of WUE supported the hypothesis that both water and nitrogen limit plant gas exchange in this semiarid environment. The short- and long-term indicators were in agreement, providing evidence in support of theoretical models concerning the water cost of carbon assimilation. 相似文献
80.
Photosensitivity of germination of white clover ( Trifolium repens L. cv. Podkowa) seeds was studied under water deficit (low water potential) conditions at 25°C. The seeds showed negative photoblastism, which was most pronounced at -0.03 MPa polyethylene glycol solution. Inhibition was observed at two different wavelength bands with maxima at 660 nm (R) and around 730 nm (FR). Red light acted identically to white light (maximum inhibition ca 50%). The effect of far-red illumination was less inhibitory (20–30%). The photoresponse required long illuminations (3 h exposures); saturation level was at 0.1 W m−2 , independently of the light quality. White clover seed germination showed no reversibility of the effects of R and FR light. Prolonged illumination with R and FR increased the inhibition, and intermittent illumination had a higher effect than a continuous one. It was concluded that the photoinhibition of germination of seeds of Trifolium repens involves a reaction dependent on the rate of phytochrome interconversion, a property that is characteristic for the high irradiance reaction. 相似文献