全文获取类型
收费全文 | 35819篇 |
免费 | 2542篇 |
国内免费 | 5068篇 |
专业分类
43429篇 |
出版年
2024年 | 189篇 |
2023年 | 777篇 |
2022年 | 1035篇 |
2021年 | 1235篇 |
2020年 | 1187篇 |
2019年 | 1710篇 |
2018年 | 1358篇 |
2017年 | 1207篇 |
2016年 | 1158篇 |
2015年 | 1090篇 |
2014年 | 1790篇 |
2013年 | 2511篇 |
2012年 | 1448篇 |
2011年 | 1635篇 |
2010年 | 1284篇 |
2009年 | 1664篇 |
2008年 | 1650篇 |
2007年 | 1819篇 |
2006年 | 1584篇 |
2005年 | 1455篇 |
2004年 | 1243篇 |
2003年 | 1190篇 |
2002年 | 1072篇 |
2001年 | 817篇 |
2000年 | 746篇 |
1999年 | 678篇 |
1998年 | 671篇 |
1997年 | 586篇 |
1996年 | 499篇 |
1995年 | 488篇 |
1994年 | 486篇 |
1993年 | 439篇 |
1992年 | 410篇 |
1991年 | 425篇 |
1990年 | 331篇 |
1989年 | 314篇 |
1988年 | 320篇 |
1987年 | 262篇 |
1986年 | 297篇 |
1985年 | 485篇 |
1984年 | 595篇 |
1983年 | 326篇 |
1982年 | 460篇 |
1981年 | 453篇 |
1980年 | 406篇 |
1979年 | 302篇 |
1978年 | 221篇 |
1977年 | 243篇 |
1976年 | 220篇 |
1973年 | 181篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
61.
Michael Brenowitz Celia Bonaventura Joseph Bonaventura Elisabetta Gianazza 《Archives of biochemistry and biophysics》1981,210(2):748-761
The hemocyanin of Limulus polyphemus is a 48-subunit aggregate. This 3.3 × 106-dalton oligomer is composed of structurally and functionally heterogeneous subunits. Using polyacrylamide electrophoresis J. Markl, A. Markl, W. Schartau, and B. Linzen (J. Comp. Physiol. Ser. B130,283–292, 1979) observed 12 bands; while using immunoelectrophoresis, M. Hoylaerts, G. Preaux, R. Witters, and R. Lontie (Arch. Int. Physiol. Biochem.87, 417–418, 1979) and J. Lamy, J. Lamy, J. Weill, J. Bonaventura, C. Bonaventura, and M. Brenowitz. (Arch. Biochem. Biophys.196, 324–339, 1979) observed 8 subunits. To proceed with an analysis of subunit roles in assembly it is first necessary to determine the number of distinct subunits. Refinement of the chromatographic separation procedures has led to the isolation of 8 immunologically distinct subunits as well as additional charge isomers which cannot be distinguished immunologically. Alkaline electrophoresis revealed 15 bands and isoelectric focusing up to 17. On the basis of extensive control experiments, including composit acrylamide-agarose immunoelectrophoresis and checks for conformational isomers, aggregation, proteolysis, and other types of degradation, we conclude that the electrophoretic heterogeneity of immunologically identical subunits is not artifactual. We have extended the nomenclature used by Lamy et al. (1979) to include the electrophoretic heterogeneity by using primes (′) to denote electrophoretically distinguishable subunits which are immunologically identical. A number of patterns have become apparent by correlating the results obtained by the different techniques. For example, immunologically pure subunit II, which shows 3 bands on alkaline electrophoresis, is in fact a mixture of electrophoretically distinct subunits II, II′, II″. Except for subunits II, II′, and II″ immunoelectrophoretically identical subunits are typically homogeneous on sodium dodecyl sulfate-gels. However, slight differences in the apparent molecular weight are observed on high-resolution gels between immunologically unrelated subunits. The immunological identity and electrophoretic differences suggest that the charge isomers which are immunologically identical have similar antigenic surfaces. If a charge substitution is not in a critical location, we would expect the electrophoretically distinct but immunologically identical subunits to have identical assembly roles. Comparison of the results for Limulus hemocyanin with the hemocyanin of related species Eurypelma californicum and Androctanus australis, which have 7 and 8 immunologically distinct subunits, respectively, suggests that the calcium-mediated aggregation from 24 to 48 subunits of Limulus does not require more extensive subunit complexity. 相似文献
62.
Caroline S. Page Franz M. Matschinsky Paule E. Lacy Susan Conant 《Biochimica et Biophysica Acta (BBA)/General Subjects》1977,497(2):408-414
Electrophysiological studies of cultured rat pancreatic β-cells using intracellular microelectrodes show that exogenous insulin over the range of 0.1–10.0 μg/ml inhibits the electrical activity due to 27.8 mM glucose in a dose-related manner. This inhibitory effect is manifested by a mean increase of the membrane potential from about ?20 to ?30 mV and inhibition of the manner of cells impaled showing spike activity from 60 to less than 10%. The inhibitory influence of insulin is rapid occuring within 5 min for the highest level used. The results provide evidence for a negative feedback role of insulin in regulating its own release. 相似文献
63.
K. Kortmulder 《Acta biotheoretica》1987,36(4):275-280
The potential and realized impact of Bohm's views on biology are briefly discussed. 相似文献
64.
L. Brzsnyi 《Biometrical journal. Biometrische Zeitschrift》1981,23(3):283-295
The paper deals with the analysis of 2 × 2 table measures and describes several new possibilities for applying them. An estimate is given for HELLMICH's asymmetric K-measure and a possible extension of the contingency concept in a special 2×2 case is presented. The concept introduced and the recommended computer application of the 2×2 table measures is supported by new results obtained for the relationship between jaw joint pains and noises examined earlier by F. SCHMID and C. ZSCHEGE. With a larger number of observations the opportunity arises for a deeper analysis of this dental problem using path analysis. The plan of evaluation and the conncetion between the variables examined is illustrated by a path-diagram. The association tables determined on the basis of the (C) values introduced during the examination of jaw joint disturbances using association analysis draw attention to the special role of the left side of mastication. The paper raises ideas for further generalisations and indicates where further research is needed. 相似文献
65.
Proteins undergoing protease reactions, heat denaturation, or interactions with sodium dodecyl sulfate (SDS) were used to demonstrate the effectiveness of a near-infrared method for the quantitative study of changes in hydration or water binding during such processes. The spectra of different proteins showed that the liberation of groups during a protease reaction is associated with a large increase in hydration and excluded volume. On the basis of experiments with model compounds, other spectral changes, including development of continuum absorbance between 1.55 and 1.85 μm and a band with a peak near 2.1 μm, were also attributed to the liberation of these groups. After heat denaturation or in the presence of SDS, the rate of proteolytic hydrolysis was markedly increased, consistent with the view that some preliminary denaturation is necessary for protease activity. The validity of the hydration changes calculated for protease reactions was supported by model studies with l-lysine, and with poly-l-lysine before and after hydrolysis. The near-infrared spectrum of the protein substrate with no added protease was largely unaffected by heat treatment alone, indicating that the hydration as such was not changed to a large extent by the structural modifications of denaturation. In contrast to the protease reaction, the interactions between SDS and the proteins resulted in a decrease in hydration. Results of this paper are compared with those obtained from other methods. Some unique advantages of the near-infrared method for the study of hydration changes during reactions in aqueous solution are described. 相似文献
66.
Frantiek Hindák 《Hydrobiologia》2000,438(1-3):107-116
Morphological variation of trichomes, heterocytes and akinetes in four species classified into the nostocalean genera Aphanizomenon Morren ex Bornet et Flahault or Anabaena Bory (Cyanophyta/Cyanobacteria) was studied from natural samples by LM and illustrated by drawings and micrographs. All investigated species, Aphanizomenon gracile (Lemmerm.) Lemmerm., A. aphanizomenoides (Forti) Komárek et Horecká, Anabaena bergii Ostenfeld, A. minderi Huber-Pestalozzi, occur in the plankton as solitary trichomes, similar to many representatives of the genus Anabaena. Main generic diagnostic characters of Aphanizomenon and Anabaena are discussed. 相似文献
67.
This paper describes a simulation problem, motivated by the study of glaucoma, a very serious and widespread ocular illness. To ascertain whether a patient suffers from glaucoma, a perimetric test is done, but the evolution of the disease is very slow, and large longitudinal sets of tests taken on the same patient are needed to study its evolution, to analyze the efficiency of existing methods to detect the progression of glaucoma and to develop new ones. Simulation can be a very useful procedure to get appropriate data sets to work with. Our aim in this work is to simulate several VFs in a healthy patient to reflect his evolution in time. We use a spatio‐temporal model to simulate from, taking into account the correlation existing between the observed (or simulated) values in space and time. Two different simulation procedures (unconditional and conditional) are studied, and applied to obtain the simulations we are interested in. (© 2004 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) 相似文献
68.
69.
Abstract. Contrary to current assumptions, the reflex blood of two-spot ladybirds, Adalia bipunctata , and seven-spot ladybirds, Coccinella septempunctata , contains haemocyte-like cells. Furthermore, DNA can be extracted and amplified from coccinellid reflex blood, confirming the presence of haemocyte-like cells and demonstrating a nondestructive method of DNA extraction. 相似文献
70.
Raphael Chijioke Njoku 《Dialectical Anthropology》2007,31(1-3):45-64
It is difficult to completely understand the life history of an intellectual excluding an understanding of his family upbringing
and formative years. Family upbringing and childhood environment, often the less known part of a life history, play crucial
roles in shaping the ideas and values individuals espouse in their adult life. Notwithstanding, this paper is not concerned
with Don C. Ohadike’s childhood. It rather focuses on the professional career of our able historian – that is the part of
his life as revealed by his most outstanding published writings. Ohadike’s published works contain a wellspring of idioms
that tell much about his values, quality of mind, and his mission as an African historian. Ohadike was a humanist, an African
patriot, and a nationalist crusader. His entire philosophy centered on safeguarding his African identity in an emergent world
of cultural imperialism.
The funds for this research were provided by a NEH-funded fellowship at the Schomburg Center, New York in the Spring of 2007.
I owe a lot of gratitude to Professor John McLeod and Dean Blaine Hudson for granting me the extra incentives to pursue my
research in New York. While all errors and misinterpretations are mine, I wish to thank the editors and anonymous reviewers
for Journal of Dialectical Anthropology for their perspective comments and suggestions on earlier drafts of this paper. 相似文献