全文获取类型
收费全文 | 1611篇 |
免费 | 146篇 |
国内免费 | 59篇 |
出版年
2024年 | 3篇 |
2023年 | 23篇 |
2022年 | 13篇 |
2021年 | 33篇 |
2020年 | 62篇 |
2019年 | 71篇 |
2018年 | 53篇 |
2017年 | 51篇 |
2016年 | 60篇 |
2015年 | 61篇 |
2014年 | 79篇 |
2013年 | 115篇 |
2012年 | 82篇 |
2011年 | 66篇 |
2010年 | 47篇 |
2009年 | 69篇 |
2008年 | 78篇 |
2007年 | 96篇 |
2006年 | 80篇 |
2005年 | 79篇 |
2004年 | 66篇 |
2003年 | 65篇 |
2002年 | 47篇 |
2001年 | 52篇 |
2000年 | 45篇 |
1999年 | 30篇 |
1998年 | 34篇 |
1997年 | 27篇 |
1996年 | 19篇 |
1995年 | 17篇 |
1994年 | 13篇 |
1993年 | 21篇 |
1992年 | 12篇 |
1991年 | 19篇 |
1990年 | 11篇 |
1989年 | 14篇 |
1988年 | 14篇 |
1987年 | 9篇 |
1986年 | 11篇 |
1985年 | 15篇 |
1984年 | 7篇 |
1983年 | 7篇 |
1982年 | 13篇 |
1981年 | 6篇 |
1980年 | 7篇 |
1978年 | 3篇 |
1977年 | 3篇 |
1976年 | 3篇 |
1974年 | 2篇 |
1971年 | 1篇 |
排序方式: 共有1816条查询结果,搜索用时 15 毫秒
101.
《Bioorganic & medicinal chemistry》2016,24(4):578-587
In this study, we evaluated the abilities of a series of chalcones to inhibit the activity of the enzyme xanthine oxidase (XO) and to scavenge radicals. 20 mono- and polyhydroxylated chalcone derivatives were synthesized by Claisen–Schmidt condensation reactions and then tested for inhibitory potency against XO, a known generator of reactive oxygen species (ROS). In parallel, the ability of the synthesized chalcones to scavenge a stable radical was determined. Structure–activity relationship analysis in conjunction with molecular docking indicated that the most active XO inhibitors carried a minimum of three hydroxyl groups. Moreover, the most effective radical scavengers had two neighboring hydroxyl groups on at least one of the two phenyl rings. Since it has been proposed previously that XO inhibition and radical scavenging could be useful properties for reduction of ROS-levels in tissue, we determined the chalcones’ effects to rescue neurons subjected to ROS-induced stress created by the addition of β-amyloid peptide. Best protection was provided by chalcones that combined good inhibitory potency with high radical scavenging ability in a single molecule, an observation that points to a potential therapeutic value of this compound class. 相似文献
102.
Eric Li C.C. Chang Zhongpu Zhang Qing Li 《Computer methods in biomechanics and biomedical engineering》2016,19(11):1210-1224
This study aims to establish a new computational framework that allows modeling transient oxygen diffusion in tissue scaffolds more efficiently. It has been well known that the survival of cells strongly relies on continuous oxygen/nutrient supply and metabolite removal. With optimal design in scaffold architecture, its ability to sustain long distance oxygen supply could be improved considerably. In this study, finite element based homogenization procedure is first used to characterize the initial effective biotransport properties in silico. These initial properties are proper indicators to prediction of the on-going performance of tissue scaffolds over time. The transient model by adopting an edge-based smoothed finite element method with combination of mass-redistributed method is then established to more efficiently simulate the transient oxygen transfer process in tissue scaffolds. The proposed new method allows large time steps to model the oxygen diffusion process without losing numerical accuracy, thereby enhancing the computational efficiency significantly, in particular for the design optimization problems which typically require numerous analysis iterations. A number of different scaffold designs are examined either under net diffusion without cell seeding, or under cellular oxygen/nutrient uptake with or without considering cell viability. The association between the homogenized effective diffusivity of net scaffold microstructures and corresponding transient diffusion and time-dependent cellular activities is divulged. This study provides some insights into scaffold design. 相似文献
103.
Strong sexual selection in males against a mutation load that reduces offspring production in seed beetles
下载免费PDF全文
![点击此处可从《Journal of evolutionary biology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Theory predicts that sexual reproduction can increase population viability relative to asexual reproduction by allowing sexual selection in males to remove deleterious mutations from the population without large demographic costs. This requires that selection acts more strongly in males than females and that mutations affecting male reproductive success have pleiotropic effects on population productivity, but empirical support for these assumptions is mixed. We used the seed beetle Callosobruchus maculatus to implement a three‐generation breeding design where we induced mutations via ionizing radiation (IR) in the F0 generation and measured mutational effects (relative to nonirradiated controls) on an estimate of population productivity in the F1 and effects on sex‐specific competitive lifetime reproductive success (LRS) in the F2. Regardless of whether mutations were induced via F0 males or females, they had strong negative effects on male LRS, but a nonsignificant influence on female LRS, suggesting that selection is more efficient in removing deleterious alleles in males. Moreover, mutations had seemingly shared effects on population productivity and competitive LRS in both sexes. Thus, our results lend support to the hypothesis that strong sexual selection on males can act to remove the mutation load on population viability, thereby offering a benefit to sexual reproduction. 相似文献
104.
105.
Carolina Ruiz Zambon Vania Helena Techio Luiz Fernando de Oliveira da Silva Adelson Francisco de Oliveira 《Plant biosystems》2019,153(1):68-76
The study evaluated the microsporogenesis of olive trees subjected to different agricultural pesticide applications during flowering. Inflorescences of cultivars Arbequina and MGS GRAP541 were subjected to agricultural pesticides: mineral oil, neem oil, dimethoate and deltamethrin. The floral buds were fixed in Carnoy for the microsporogenesis analysis and in Karnovsky for scanning electron microscopy. The slides were prepared by squash technique and staining with propionic carmine. The pollen viability was determined by Alexander’s stain and in vitro germination. Results show that the quantification of abnormalities in meiosis in the two cultivars caused significant effect among the treatments, being that all differed statistically from the control group. Both methods showed a higher percentage of viable pollens in the control treatment and lower percentage of viability with the agricultural pesticides. The method of pollen viability by staining presented the highest averages of viable pollens, but when compared together, both methods presented a strongly related positive linear correlation. It was concluded that the used chemical products increased the percentage of chromosomal abnormalities during microsporogenesis, which interfered in the pollen viability of the two analyzed cultivars. The product deltamethrin caused the strongest effect on meiosis and on pollen viability. 相似文献
106.
Ramzi Al-Sarhan Nuru Adgaba Yilma Tadesse Yehya Alattal Amal Al-Abbadi Arif Single Ahmad Al-Ghamdi 《Saudi Journal of Biological Sciences》2019,26(7):1581-1586
The current study aimed to investigate the important reproductive biology and morphology of A.m. jemenitica queens and drones through measuring the weight of virgin and mated queens, size and weight of spermathecae, weight of ovaries, number of ovarioles, quantity and viability of semen in queen and drones. Accordingly, the average weights of 0.139 ± 0.01 g and 0.143 ± 0.013 g recorded for virgin and mated queens respectively. The sizes of spermathecae were 1.248 ± 0.103 mm and 1.25 ± 0.022 mm for virgin and mated queens respectively. The mean weight of ovaries was 0.013 ± 0.003 g and the numbers of ovarioles varied from 124 to 163 with the mean of 142.9 ± 9.47 and with no significant difference between virgin and mated queens. The average number of stored sperm per spermathecae of mated queen was estimated to be 4.202 ± 0.613 million with the viability of 80.39%. The average number of sperm per drone recorded was 8,763,950 ± 1,633,203.15 with viability of 79.54 ± 6.70%. In general, the current study revealed that the values recorded for reproductive biology and morphological characters of A. m. jemenitica queens and drones were relatively lower than values recorded for other Apis mellifera races. This mainly could be associated with the body size of the race which is known to be the smallest race among A. mellifera races. Moreover, the harsh environmental conditions of the regions, high temperature, low humidity and limited resources may have contributed for the smaller biological and morphological values. The information will serve as a base in future selection and breeding of program of the race. 相似文献
107.
Forbs are important biodiversity components of grasslands and are often threatened by management intensification. As most forbs propagate predominantly by seed, knowledge of their seed regenerative traits would improve the conservation and restoration of forb-rich ecosystems. The main seed production traits of six forbs that are common in European species-rich grasslands were studied by collecting fertile shoots from different sites and over several years. Among sites and years, variability was high, particularly in the number of inflorescences per shoot, which affected ovule production more than any other trait. Relationships between inflorescence size and the number of ovules were mainly negatively allometric or almost so, with lower flower densities in larger inflorescences. The average ovule-to-seed transformation efficiency was 58%. There was significant variation among collections of the same species, and even more between species. Species with a low ovule-to-seed transformation efficiency generally exhibited compensatory, high seed viability. Large inflorescences had high ovule to seed utilization values, probably because of better nutrient conditions. Seed germinability (average, 30%) was much lower than seed viability (average, 54%); therefore, seed dormancy was an important feature of the species studied. 相似文献
108.
Direct analysis of pollen fitness by flow cytometry: implications for pollen response to stress 总被引:1,自引:0,他引:1
Gilad Luria Nicholas Rutley Itay Lazar Jeffery F. Harper Gad Miller 《The Plant journal : for cell and molecular biology》2019,98(5):942-952
Sexual reproduction in flowering plants depends on the fitness of the male gametophyte during fertilization. Because pollen development is highly sensitive to hot and cold temperature extremes, reliable methods to evaluate pollen viability are important for research into improving reproductive heat stress (HS) tolerance. Here, we describe an approach to rapidly evaluate pollen viability using a reactive oxygen species (ROS) probe dichlorodihydrofluorescein diacetate (i.e. H2DCFDA‐staining) coupled with flow cytometry. In using flow cytometry to analyze mature pollen harvested from Arabidopsis and tomato flowers, we discovered that pollen distributed bimodally into ‘low‐ROS’ and ‘high‐ROS’ subpopulations. Pollen germination assays following fluorescence‐activated cell sorting revealed that the high‐ROS pollen germinated with a frequency that was 35‐fold higher than the low‐ROS pollen, supporting a model in which a significant fraction of a flower's pollen remains in a low metabolic or dormant state even after hydration. The ability to use flow cytometry to quantify ROS dynamics within a large pollen population was shown by dose‐dependent alterations in DCF‐fluorescence in response to oxidative stress or antioxidant treatments. HS treatments (35°C) increased ROS levels, which correlated with a ~60% reduction in pollen germination. These results demonstrate the potential of using flow cytometry‐based approaches to investigate metabolic changes during stress responses in pollen. 相似文献
109.
Afrotropical ant-following birds are vulnerable to forest loss and disturbance, but critical habitat thresholds regarding their abundance and species richness in human-dominated landscapes, including industrial oil palm plantations, have never been assessed. We measured forest cover through Landsat imagery and recorded species richness and relative abundance of 20 ant-following birds in 48 plots of 1-km2, covering three landscapes of Southwest Cameroon: Korup National Park, smallholder agroforestry areas (with farms embedded in forest), and an industrial oil palm plantation. We evaluated differences in encounter frequency and species richness among landscapes, and the presence of critical thresholds through enhanced adaptive regression through hinges. All species were detected in Korup National Park and the agroforestry landscape, which had similar forest cover (>85%). Only nine species were found in the oil palm plantation (forest cover = 10.3 ± 3.3%). At the 1-km2 scale, the number of species and bird encounters were comparable in agroforests and the protected area: mean species richness ranged from 12.2 ± 0.6 in the park and 12.2 ± 0.6 in the agroforestry matrix to 1.0 ± 0.4 in the industrial oil palm plantation; whereas encounters decreased from 34.4 ± 3.2 to 26.1 ± 2.9 and 1.3 ± 0.4, respectively. Bird encounters decreased linearly with decreasing forest cover, down to an extinction threshold identified at 24% forest cover. Species richness declined linearly by ca. one species per 7.4% forest cover lost. We identified an extinction threshold at 52% forest cover for the most sensitive species (Criniger chloronotus, Dicrurus atripennis, and Neocossyphus poensis). Our results show that substantial proportions of forests are required to sustain complete ant-following bird assemblages in Afrotropical landscapes and confirm the high sensitivity of this bird guild to deforestation after industrial oil palm development. Securing both forest biodiversity and food production in an Afrotropical production landscape may be best attained through a combination of protected areas and wildlife-friendly agroforestry. 相似文献
110.
Tom Van der Stocken Alison K. S. Wee Dennis J. R. De Ryck Bram Vanschoenwinkel Daniel A. Friess Farid Dahdouh‐Guebas Marc Simard Nico Koedam Edward L. Webb 《Biological reviews of the Cambridge Philosophical Society》2019,94(4):1547-1575
Dispersal allows species to shift their distributions in response to changing climate conditions. As a result, dispersal is considered a key process contributing to a species' long‐term persistence. For many passive dispersers, fluid dynamics of wind and water fuel these movements and different species have developed remarkable adaptations for utilizing this energy to reach and colonize suitable habitats. The seafaring propagules (fruits and seeds) of mangroves represent an excellent example of such passive dispersal. Mangroves are halophytic woody plants that grow in the intertidal zones along tropical and subtropical shorelines and produce hydrochorous propagules with high dispersal potential. This results in exceptionally large coastal ranges across vast expanses of ocean and allows species to shift geographically and track the conditions to which they are adapted. This is particularly relevant given the challenges presented by rapid sea‐level rise, higher frequency and intensity of storms, and changes in regional precipitation and temperature regimes. However, despite its importance, the underlying drivers of mangrove dispersal have typically been studied in isolation, and a conceptual synthesis of mangrove oceanic dispersal across spatial scales is lacking. Here, we review current knowledge on mangrove propagule dispersal across the various stages of the dispersal process. Using a general framework, we outline the mechanisms and ecological processes that are known to modulate the spatial patterns of mangrove dispersal. We show that important dispersal factors remain understudied and that adequate empirical data on the determinants of dispersal are missing for most mangrove species. This review particularly aims to provide a baseline for developing future research agendas and field campaigns, filling current knowledge gaps and increasing our understanding of the processes that shape global mangrove distributions. 相似文献