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排序方式: 共有1961条查询结果,搜索用时 31 毫秒
91.
92.
93.
Sandra Schlee Kristina Straub Thomas Schwab Thomas Kinateder Rainer Merkl Reinhard Sterner 《Proteins》2019,87(10):815-825
It is an important goal of computational biology to correctly predict the association state of a protein based on its amino acid sequence and the structures of known homologues. We have pursued this goal on the example of anthranilate phosphoribosyltransferase (AnPRT), an enzyme that is involved in the biosynthesis of the amino acid tryptophan. Firstly, known crystal structures of naturally occurring homodimeric AnPRTs were analyzed using the Protein Interfaces, Surfaces, and Assemblies (PISA) service of the European Bioinformatics Institute (EBI). This led to the identification of two hydrophobic “hot spot” amino acids in the protein-protein interface that were predicted to be essential for self-association. Next, in a comprehensive multiple sequence alignment (MSA), naturally occurring AnPRT variants with hydrophilic or charged amino acids in place of hydrophobic residues in the two hot spot positions were identified. Representative variants were characterized in terms of thermal stability, enzymatic activity, and quaternary structure. We found that AnPRT variants with charged residues in both hot spot positions exist exclusively as monomers in solution. Variants with hydrophilic amino acids in one hot spot position occur in both forms, monomer and dimer. The results of the present study provide a detailed characterization of the determinants of the AnPRT monomer-dimer equilibrium and show that analysis of hot spots in combination with MSAs can be a valuable tool in prediction of protein quaternary structures. 相似文献
94.
Bjrn Rrslett 《International Review of Hydrobiology》1987,72(1):1-25
The underwater light field is described as a stochastic process, with the vertical attenuation as a random variable. It is shown that the vertical attenuation integral is well described as a Normal process with uncorrelated increments. The attenuation processes within a water body are found to be quite independent of the incoming irradiance. Thus, the relative light intensity at any depth can be approximated by a Lognormal random variable. Based upon the expectation of this Lognormal variable, and the mean value of the incident irradiance, the irradiance delivered at any depth can be estimated, as well as the statistical distribution of the irradiance. Light data from a number of Norwegian soft-water lakes showed good fit to the model. Two productive lakes included in this study also had a light regime well described by the statistical model. However, the model should be extended to cater for seasonal variations in these applications. 相似文献
95.
Don Maurer Richard T. Keck Jeffrey C. Tinsman Wayne A. Leathem Christian Wethe Charles Lord Thomas M. Church 《International Review of Hydrobiology》1986,71(1):49-63
This account describes the comparative response of four species of benthic invertebrates to burial in terms of vertical migration and mortality, and provides a synthesis of studies and recommendations upon which to assess future impacts. The species featured were the bivalve Mercenaries mercenaria, the amphipod crustacean Parahaustorius longimerus, and the polychaetes Scoloplos fragilis and Nereis succinea. There was evidence of synergistic effects on burrowing activity and mortality with changes in time of burial, sediment depth, sediment type and temperature. In sediment with silt-clay, N. succinea was the most resistant species followed by M. mercenaria, S. fragilis and P. longimerus. In sediment without silt-clay the order of percent mortality was reversed. Studies of surface water chemistry and sediment geochemistry showed that dissolved oxygen decreased significantly and ammonia and sulfide increased significantly between the surface and below 2.0 cm within a 15-day period. Based on these results and physiological tolerances from the literature it was concluded that M. mercenaria and N. succinea would be relatively resistant to chemical effects of spoil disposal, whereas S. fragilis and P. longimerus would be less resistant to such effects. Vertical migration of benthic invertebrates through dredge disposal can be a viable mechanism of recolonization under certain conditions. Some effects of burial of benthos can be predicted based on morphology, behavior and physiology. These biological features were discussed with examples dealing with molluscs, crustaceans, and polychaetes. Finally, recommendations were made concerning the type of studies to provide additional data to aid management agencies in decision making about future dredging and disposal practices. 相似文献
96.
Daniel M. Dauer R. Michael Ewing Anthony J. Rodi 《International Review of Hydrobiology》1987,72(5):529-538
The distribution of the macrobenthic infaunal community within the upper 25 cm of the sediment was studied at 16 stations in the lower Chesapeake Bay. Stations were located from the tidal freshwater to the polyhaline zone of major tributaries (James, York and Rappahannock Rivers) and in the polyhaline portion of the lower bay mainstem. Profiles for total number of individuals, total ash-free dry weight biomass and species encountered with depth were calculated. Except for the deep dwelling bivalve, Macoma balthica, tributary macrobenthic communities had a shallow depth distribution compared to the mainstem sites which were found in generally coarser sediments in the higher salinity region of the estuary. 相似文献
97.
98.
Miguel Alvarez Cobelas 《International Review of Hydrobiology》1991,76(2):213-223
The underwater light field has been studied in a hypertrophic, gravel-pit lake close to Madrid (Spain) during a one year cycle. Both the inherent and the apparent properties of the underwater light field have been weekly surveyed. As theoretically expected, there is a link between inherent and apparent properties in this lake. Evidence is given suggesting that a seasonal trend in the underwater light field seems to occur. The main factor attenuating light in the vertical column is phytoplankton chlorophyll “a” but humic substances also appear to play an importtant role in light attenuation. 相似文献
99.
Celia VinagreIzquierdo Kasun H. Bodawatta Krytof Chmel Justinn ReneliesHamilton Luda Paul Pavel Munclinger Michael Poulsen Knud A. Jnsson 《Ecology and evolution》2022,12(2)
Haemosporidians are among the most common parasites of birds and often negatively impact host fitness. A multitude of biotic and abiotic factors influence these associations, but the magnitude of these factors can differ by spatial scales (i.e., local, regional and global). Consequently, to better understand global and regional drivers of avian‐haemosporidian associations, it is key to investigate these associations at smaller (local) spatial scales. Thus, here, we explore the effect of abiotic variables (e.g., temperature, forest structure, and anthropogenic disturbances) on haemosporidian prevalence and host–parasite networks on a horizontal spatial scale, comparing four fragmented forests and five localities within a continuous forest in Papua New Guinea. Additionally, we investigate if prevalence and host–parasite networks differ between the canopy and the understory (vertical stratification) in one forest patch. We found that the majority of Haemosporidian infections were caused by the genus Haemoproteus and that avian‐haemosporidian networks were more specialized in continuous forests. At the community level, only forest greenness was negatively associated with Haemoproteus infections, while the effects of abiotic variables on parasite prevalence differed between bird species. Haemoproteus prevalence levels were significantly higher in the canopy, and an opposite trend was observed for Plasmodium. This implies that birds experience distinct parasite pressures depending on the stratum they inhabit, likely driven by vector community differences. These three‐dimensional spatial analyses of avian‐haemosporidians at horizontal and vertical scales suggest that the effect of abiotic variables on haemosporidian infections are species specific, so that factors influencing community‐level infections are primarily driven by host community composition. 相似文献
100.
Ann Vanreusel Magda Vincx Danielle Schram Dirk van Gansbeke 《International Review of Hydrobiology》1995,80(2):313-326
The metazoan meiofauna of nine stations in shelf break and upper slope areas (70 to 1500 m water depth) of the N.E. Atlantic were investigated in order to assess which environmental factors are important in the control of densities and sediment profiles. Total meiofaunal densities (ranging between 368 and 1523 ind/10 cm2) were correlated with bacterial densities, an important food source for meiofauna. However, considering sediment vertical distribution profiles, the relative importance of both food and oxygen on the meiofauna became obvious. A combination of both bacterial densities and oxygen supply could explain about 95% of the variability in the vertical profiles of the meiofauna densities. Meiofauna numbers increase in proportion to food availability in the surface sediment layers, but this relationship breaks down in deeper sediment layers where the oxygen supply is often limiting, particularly in fine sediments. 相似文献