全文获取类型
收费全文 | 5525篇 |
免费 | 436篇 |
国内免费 | 1034篇 |
专业分类
6995篇 |
出版年
2024年 | 31篇 |
2023年 | 106篇 |
2022年 | 125篇 |
2021年 | 161篇 |
2020年 | 219篇 |
2019年 | 202篇 |
2018年 | 217篇 |
2017年 | 199篇 |
2016年 | 229篇 |
2015年 | 227篇 |
2014年 | 309篇 |
2013年 | 501篇 |
2012年 | 216篇 |
2011年 | 297篇 |
2010年 | 245篇 |
2009年 | 321篇 |
2008年 | 306篇 |
2007年 | 311篇 |
2006年 | 320篇 |
2005年 | 244篇 |
2004年 | 225篇 |
2003年 | 227篇 |
2002年 | 179篇 |
2001年 | 172篇 |
2000年 | 128篇 |
1999年 | 125篇 |
1998年 | 95篇 |
1997年 | 80篇 |
1996年 | 97篇 |
1995年 | 84篇 |
1994年 | 72篇 |
1993年 | 88篇 |
1992年 | 56篇 |
1991年 | 91篇 |
1990年 | 44篇 |
1989年 | 47篇 |
1988年 | 37篇 |
1987年 | 37篇 |
1986年 | 39篇 |
1985年 | 34篇 |
1984年 | 46篇 |
1983年 | 34篇 |
1982年 | 35篇 |
1981年 | 28篇 |
1980年 | 18篇 |
1979年 | 18篇 |
1978年 | 18篇 |
1977年 | 13篇 |
1976年 | 11篇 |
1975年 | 7篇 |
排序方式: 共有6995条查询结果,搜索用时 13 毫秒
21.
Hanna M. Pappius 《Neurochemical research》1991,16(9):941-949
The studies reviewed here represent a continuing search for mechanisms which play a role in neurological disturbances resulting from brain injury. Focal cortical freezing lesions in rats were shown to cause a widespread decrease in local cerebral glucose utilization (LCGU) in cortical areas of the lesioned hemisphere and this was interpreted as reflecting a depression of cortical activity. Such an interpretation was supported by the finding that in lesioned brain reduction of cerebral metabolism by pentobarbital and isoflurane was limited by the metabolic depression that has already occurred as a result of injury and by the demonstration that the energy status and substrate (glucose) supply in the cortical areas in the injured brain have not been compromised at the time when LCGU was decreased. Both the serotonergic and the noradrenergic neurotransmitter systems were implicated in functional alterations associated with injury. Cortical serotonin (5-HT) metabolism was increased throughout the lesioned hemisphere and complete inhibition of 5-HT synthesis withp-chlorophenylalanine ameliorated the decrease in cortical LCGU, interpreted as reflecting cortical functional depression. Cortical norepinephrine metabolism was bilaterally increased in focally injured brain, while prazosin, a selective 1-noradrenergic receptor blocker, normalized cortical LCGU in the lesioned hemisphere. Low-affinity in vivo binding of [125I]HEAT, another selective 1-receptor ligand, was specifically increased in cortical areas of the lesioned hemisphere at the time of the greatest depression in LCGU, suggesting that 1-adrenoreceptors may be of functional importance in injured brain. The general conclusion from this series of studies on mechanisms underlying functional disturbances in injured brain is that both the serotonergic and the noradrenergic neurotransmitter systems are involved in the widespread cortical depression which develops with time as a consequence of a focal lesion. The data are compatible with the inhibitory effects of NE and 5-HT in the cortex and with the hypothesis that these two transmitter systems affect cortical information processing. 相似文献
22.
A bioluminescent assay for the determination of phosphoenolpyruvate carboxykinase activity in nanogram-sized tissue samples 总被引:1,自引:0,他引:1
Monika Wimmer 《Analytical biochemistry》1988,170(2):376-381
A highly specific and sensitive assay for the determination of phosphoenolpyruvate carboxykinase (PEPCK) in nanogram-sized tissue samples is described. This test system is based on the stoichiometric transformation of phosphoenolpyruvate into ATP. In a subsequent step ATP is quantified by bioluminescent techniques. The applicability of this assay system is shown by measurements in liver samples with normal and high PEPCK activity levels. 相似文献
23.
M. A. Khan 《Hydrobiologia》1986,135(3):233-242
L. Naranbagh (alt. 1587 m) is a polymictic, shallow marl lake in the flood-plain valley of Kashmir, India. Macrofloral affinities resemble Potamogeton Type of Forsberg (1965) with alkaline waters, not rich in phosphorus. CaCO3 precipitation coupled with decline in Ca2+ and alkalinity values are characteristic of the lake. Fluctuations in Mg2+, Na+, K+, and Cl– were relatively conservative. The levels of PO
inf4
sup3–
-P and NO
inf3
sup–
-N indicate moderate fertility of the lake water.Persistence of a summer-autumn planktonic algal pulse is related to favourable irradiance, high water temperatures, and increased photosynthetic efficiency values. The most striking seasonality in photosynthetic rates (m–2 h–1) between winter minimum (3 mg Cassim) and summer maximum (75.4 mg Cassim) is determined by mainly climatic changes. Energy flow gave annual phytoplankton production of 51.95 × 102 KJ m–2 for the ecosystem.The nutrient levels and productivity rates suggest mesotrophic status of L. Naranbagh in classic oligoeutrophic classification of lake types. 相似文献
24.
Abstract A most-probable-number technique was used to quantify the abundance of myxomycetes (myxogastrids) in soil samples taken from 23 'Sites of Special Scientific Interest' and one other site in the West of England. Associated organisms and soil conditions were also recorded. Sixteen of the 24 sites yielded myxomycete plasmodium-forming units (PFUs) in numbers averaging 230 cm−3 fresh soil. Where detectable in samples of grassland soil, the numbers ranged from 20–2750 cm−3 , in woodland soil from 20–800 cm−3 , and in sand dunes from 80–400 cm−3 . Repeated sampling revealed changing numbers at single sites. The abundance of PFUs was correlated positively with numbers of soil amoebae, ciliates and nematodes, and with levels of magnesium and soil density, and negatively with organic matter content and levels of NH4 -nitrogen and phosphate. Each PFU probably corresponded to one or several uninucleate cells in the soil rather than to a plasmodium. 相似文献
25.
Estimation in change-point hazard rate models 总被引:1,自引:0,他引:1
26.
A functional differential equation which is nonlinear and involves forward and backward deviating arguments is solved numerically. The equation models conduction in a myelinated nerve axon in which the myelin completely insulates the membrane, so that the potential change jumps from node to node. The equation is of first order with boundary values given at t=±. The problem is approximated via a difference scheme which solves the problem on a finite interval by utilizing an asymptotic representation at the endpoints, cubic interpolation and iterative techniques to approximate the delays, and a continuation method to start the procedure. The procedure is tested on a class of problems which are solvable analytically to access the scheme's accuracy and stability, then applied to the problem that models propagation in a myelinated axon. The solution's dependence on various model parameters of physical interest is studied. This is the first numerical study of myelinated nerve conduction in which the advance and delay terms are treated explicitly.Supported in part by NSF Grant MCS8301724 and by a Biomedical Research Support Grant 2SO7RR0706618 from NIH 相似文献
27.
Summary An analysis of the preoptic area of the lizard, Lacerta sicula R., with the use of the Golgi method revealed that: 1)in principle, the dendritic pattern of its neurons is relatively simple; 2) the supraoptic nucleus contains large- to medium-sized bipolar or multipolar neurons together with small, usually multipolar nerve cells; 3) the preoptic periventricular gray and the paraventricular nucleus exhibit a varied neuronal typology, including large multipolar or bipolar elements, abundant CSF-contacting neurons, and some tufted elements; and 4) the lateral regions display some conspicuous multipolar neurons.With a financial contribution from Ministero della Pubblica Istruzione 相似文献
28.
János Podani 《Plant Ecology》1989,83(1-2):111-128
The methodology of comparing the results of multivariate community studies (resemblance matrices, ordinations, hierarchical and nonhierarchical classifications) is reviewed from two viewpoints: basic strategy and measure employed. The basic strategy is determined by 7 choices concerning the type of results, consensus methods or resemblance measures, hypothesis testing or exploratory analysis, lack or presence of reference basis, data set congruence or algorithmic effects, number of factors responsible for differences among results, and the number of properties considered in the comparison. Included is a brief summary of methods applicable to vegetation studies. Examples from a grassland survey demonstrate the utility of comparisons in evaluating the effects of plot size, data type, standardization, taxonomic level and number of species on classifications and ordinations.Abbreviations OUC =
Operational Unit of Comparison
- PCA =
Principal Components Analysis
- PCoA =
Principal Coordinates Analysis
- SSA =
Incremental Sum of Squares Agglomeration 相似文献
29.
鱼类染色体G—显带的Brd U—BSG方法及白鲢G—带模式图的初步建立 总被引:6,自引:2,他引:4
本文报道了一种显示鱼类染色体G-带的BrdU-BsG方法。采用肾细胞短期培养,收获前12小时加入BrdU,使终浓度为10μg/ml。制片经HCl、Ba(OH)_2处理,4×SSC温育。Giemsa染色,显示出白鲢的G-带。其带纹细致清晰,一个细胞的单倍染色体上显示带纹达200条以上,是目前已报道的鱼类多重带中带纹最多的,且反差明显,带纹有特征性,结果较稳定。根据实验结果初步建立了白鲢的G-带模式图。 相似文献
30.
A stereological method for estimating fish fecundity 总被引:1,自引:0,他引:1
This paper describes the application and evaluation of a stereological method for counting fish oocytes. The method has been applied to herring, Clupea harengus L., Dover sole, Solea solea L., and mackerel, Scomber scombrus L., and compared with existing volumetric and/or automated particle counter methods. The stereological method has been shown to given similar results and to have a number of distinct advantages over the other methods. 相似文献