全文获取类型
收费全文 | 1202篇 |
免费 | 69篇 |
国内免费 | 55篇 |
专业分类
1326篇 |
出版年
2024年 | 5篇 |
2023年 | 15篇 |
2022年 | 13篇 |
2021年 | 27篇 |
2020年 | 23篇 |
2019年 | 40篇 |
2018年 | 35篇 |
2017年 | 20篇 |
2016年 | 42篇 |
2015年 | 37篇 |
2014年 | 47篇 |
2013年 | 87篇 |
2012年 | 41篇 |
2011年 | 82篇 |
2010年 | 39篇 |
2009年 | 66篇 |
2008年 | 58篇 |
2007年 | 73篇 |
2006年 | 58篇 |
2005年 | 42篇 |
2004年 | 38篇 |
2003年 | 37篇 |
2002年 | 38篇 |
2001年 | 23篇 |
2000年 | 41篇 |
1999年 | 29篇 |
1998年 | 15篇 |
1997年 | 24篇 |
1996年 | 19篇 |
1995年 | 15篇 |
1994年 | 15篇 |
1993年 | 24篇 |
1992年 | 14篇 |
1991年 | 12篇 |
1990年 | 9篇 |
1989年 | 14篇 |
1988年 | 15篇 |
1987年 | 10篇 |
1986年 | 8篇 |
1985年 | 14篇 |
1984年 | 11篇 |
1983年 | 8篇 |
1982年 | 9篇 |
1981年 | 9篇 |
1980年 | 8篇 |
1979年 | 4篇 |
1975年 | 2篇 |
1974年 | 4篇 |
1973年 | 3篇 |
1950年 | 1篇 |
排序方式: 共有1326条查询结果,搜索用时 0 毫秒
101.
Dihydrocaffeic acid, a dietary constituent and a microbial metabolite of flavonoids, is an antioxidant, but few biological effects have been examined. After its production by microflora in the colon, dihydrocaffeic acid is absorbed and found in plasma as a combination of free and metabolized forms. Excess solar UV radiation provokes damage and initiates immune response and inflammation in skin, sometimes leading to cancer. Dihydrocaffeic acid reduced the cytotoxicity and pro-inflammatory cytokine production (interleukin-6 and -8) in HaCaT cells, a keratinocyte model, following UV radiation. The effect of dihydrocaffeic acid may result from a combination of direct radical scavenging of the reactive oxygen species formed or reinforcement of the antioxidant potential of the keratinocytes, as well as a direct interference with the pathway involved in cytokine stimulation. The minimum structure required for such an effect appears to consist of a propionate side chain attached to a catechol moiety, as indicated by the efficacy of caffeic acid, but not of the methyl and glucuronide conjugates of dihydrocaffeic acid. The data obtained suggest that dihydrocaffeic acid is a potential candidate for photo-protection by interfering with the events initiated after UV exposure in keratinocytes. 相似文献
102.
Liu Q He X Liu Y Du B Wang X Zhang W Jia P Dong J Ma J Wang X Li S Zhang H 《Biochemical and biophysical research communications》2008,377(3):775-779
Oxidative damage is an important mechanism in X-ray-induced cell death. Radiolysis of water molecules is a source of reactive oxygen species (ROS) that contribute to X-ray-induced cell death. In this study, we showed by ROS detection and a cell survival assay that NADPH oxidase has a very important role in X-ray-induced cell death. Under X-ray irradiation, the upregulation of the expression of NADPH oxidase membrane subunit gp91phox was dose-dependent. Meanwhile, the cytoplasmic subunit p47phox was translocated to the cell membrane and localized with p22phox and gp91phox to form reactive NADPH oxidase. Our data suggest, for the first time, that NADPH oxidase-mediated generation of ROS is an important contributor to X-ray-induced cell death. This suggests a new target for combined gene transfer and radiotherapy. 相似文献
103.
We studied the effects of irradiation with X-rays (the total dose of 0.0129 C/kg was attained over 7, 14, or 21 days), increased entry of Al3+ into the organism (0.2% AlCl3 in drinking water), and the combined influence of these factors for 21 days on the contents of the soluble and filamentous forms of glial fibrillary acidic protein (GFAP) in the tissues of the hippocampus, cerebellum, and neocortex of albino rats. After irradiation for 7 days, a clear trend toward drops in the GFAP contents in the structures under study was observed, while irradiation in the same dose, but for 14 or 21 days, resulted in increases in the contents of both GFAP forms (within a range of 13-29%, as compared with the control). Entry of aluminum chloride with water also resulted in an increase in the GFAP contents in all studied structures; changes in the filamentous form were more intensive. The combined influence of irradiation and Al3+ resulted in more intensive shifts in the GFAP levels; the content of its filamentous form increased in all structures by about 50%, while shifts of the soluble form were somewhat smaller. 相似文献
104.
We produced mutants resistant to Alternaria blotch disease in several cultivars of apple (Malus × domestica Borkh.) by irradiation with X- or γ-rays. An efficient in vitro assay method was established using chemically-synthesized AM-toxin I of Alternaria alternata (Fr.) Keissler to screen for mutants resistant to Alternaria blotch disease. The frequency of necrotic lesions was investigated by applying various concentrations of AM-toxin I to leaf discs of the first, third, and fifth leaves from the shoot apex of several apple cultivars, including Jonathan, Fuji, Oorin, and Indo. In vitro-grown apple shoots of susceptible cultivars were then treated with various doses of X- or γ-ray irradiation. Several mutants resistant to AM-toxin I were obtained by combining the techniques for tissue culture of apple shoots with the AM-toxin I screening method. Following a repeat second screening test with AM-toxin I, mutant plants were sprayed with a spore suspension of A. alternata and found resistant to be the fungal pathogen. These mutants showed normal phenotypic appearance, and so far, no difference has been observed between the original plants and mutants except for the susceptibility to Alternaria blotch. 相似文献
105.
The culture liquid of Luteococcus japonicus subsp. casei was found to be able to reactivate cells of this bacterium inactivated by UV irradiation or heat shock. The antistress activity of the culture liquid was due to the presence of an extracellular exometabolite of a protein nature with a molecular mass of more than 10 kDa. When the bacterium was grown in a nutrient broth or glucose-containing mineral medium, the antistress protein was secreted by cells in the logarithmic growth phase. The reactivating effect of the antistress protein was inversely proportional to the survival rate of stressed cells. 相似文献
106.
Wen Guang Bates T. E. Inanaga S. Voroney R. P. Hamamura K. Curtin D. 《Plant and Soil》2002,246(2):241-248
Organic wastes such as sewage sludges contain copper (Cu). Increased attention to environmental protection requires that wastes be treated with pathogen-eliminating procedures before application to farmland. It is not clear, however, if such procedures affect the plant availability of Cu in the wastes. This 2-year field research investigated the effect of irradiation and composting on Cu availability in sludges and manure using a yield control approach. Four organic wastes [digested and dewatered (DSS), digested and irradiated (DISS), composted (DICSS) sewage sludges and composted livestock manure (CLM)] were applied at four rates (10, 20, 30 and 40 t solid ha–1 year–1) with supplemented N and K fertilizers. A control treatment (CT) received N and K fertilizers only. Beans, lettuce and petunias were grown in first year and lettuce were harvested twice in second year. Beans appeared to have a strong ability to absorb Cu compared with the other test crops. In general, crop Cu concentration responses to Cu applied in DSS and DISS were well described by quadratic equations. Tested by a paired t-test, Cu concentration in bean pods was higher in DISS than in DSS treatment, indicating that irradiation increased phytoavailability of Cu. However, the parabolic response of crop Cu to Cu applied in DISS, suggesting that the increases were confined to the lower rates of DISS. Copper applied in DICSS did not increase Cu concentration in any of the test crops. At a given level of applied Cu, crop Cu tended to be lower in DICSS than in DSS or DISS treatment. Sludge composting depressed phytoavailability of Cu. Copper concentration in CLM was much lower than in sludges and sludge composts, but application of CLM increased Cu concentration in bean pods. The pattens of Cu concentration in the two cuts of lettuce in 1991 to Cu applied in CLM were similar, where low rates of CLM application slightly reduced Cu concentration, then the Cu concentration increased with increased rates. The dynamics of available Cu supply were different in the sludge composts and manure composts. 相似文献
107.
Summary To explain the decline of Hippopha? scrub in the vegetation succession in the dunes of The Netherlands, the growth and nodulation
of Hippopha? plants grown in pots, using soil from an early stage (site AH) and a post-optimum stage (site HP), were investigated.
In HP-soil nodulation, yield, and the nitrogen and phosphorus content of test plants were always lower and the number of necrotic
nodules and the dry matter content were always higher than in AH-soil, even after inoculation with crushed nodules and the
addition of a nutrient solution. Plants in HP-soil also had darker roots, less root hairs, a higher number of short lateral
roots and a higher percentage of dead roots than those in AH-soil. These characteristics of adverse growth conditions disappeared
upon ignition or gamma-irradiation of HP-soil.
Possible explanations of these results are discussed. The degeneration of Hippopha? scrub cannot be ascribed to the age of
the plants, the absence of sufficient infective endophyte particles or to abiotic factors such as unfavourable physical (particle
size) or chemical soil conditions but is caused by biotic factors. No indications were obtained that plant-pathogenic fungi
and bacteria are involved. HP-soil in contrast to AH-soil, however, contained large numbers of the nematodeLongidorus sp., a species known to cause root deformations. The conclusion was that this nematode is one of the biotic factors involved
in the degeneration of the Hippopha? scrub. This degeneration is due to a restriction of the root system resulting in a low
phosphate uptake, a low nodulation capacity and, as a consequence, a low nitrogen content. The results demonstrate that biotic
soil factors are important in influencing succession in higher plant communities. 相似文献
108.
Photosystem I preparations were irradiated with UV to destroy vitamin K1 in situ. The depletion of vitamin K1 resulted in inactivation of NADP+ photoreduction and introduction of a 220 ms component in the flash generated P700+ re-reduction at room temperature. The photoreduction of the terminal FeS centers FA and FB in control and vitamin K1-depleted preparations at 7 K were comparable. The data confirm that vitamin K1 is functionally implicated in primary electron transfer reactions in PS I at physiological temperature, and that the anomalous results at cryogenic temperature may be explicable in terms of a by-pass of the vitamin K1 acceptor site or heterogeneity introduced into the photosystem by quinone removal. 相似文献
109.
110.
The CNS (central nervous system) is unquestionably the central organ that regulates directly or indirectly all physiological systems in the mammalian body. Yet, when considering the defence of the CNS from pathogens, the CNS has often been considered passive and subservient to the pro-inflammatory responses of the immune system. In this view, neuroinflammatory disorders are examples of when the tail (the immune system) wags the dog (the CNS) to the detriment of an individual''s function and survival. 相似文献