全文获取类型
收费全文 | 8136篇 |
免费 | 415篇 |
国内免费 | 242篇 |
专业分类
8793篇 |
出版年
2024年 | 27篇 |
2023年 | 63篇 |
2022年 | 84篇 |
2021年 | 79篇 |
2020年 | 183篇 |
2019年 | 230篇 |
2018年 | 242篇 |
2017年 | 174篇 |
2016年 | 157篇 |
2015年 | 155篇 |
2014年 | 624篇 |
2013年 | 846篇 |
2012年 | 496篇 |
2011年 | 404篇 |
2010年 | 266篇 |
2009年 | 338篇 |
2008年 | 335篇 |
2007年 | 403篇 |
2006年 | 329篇 |
2005年 | 353篇 |
2004年 | 236篇 |
2003年 | 241篇 |
2002年 | 205篇 |
2001年 | 201篇 |
2000年 | 135篇 |
1999年 | 154篇 |
1998年 | 177篇 |
1997年 | 144篇 |
1996年 | 160篇 |
1995年 | 211篇 |
1994年 | 203篇 |
1993年 | 163篇 |
1992年 | 146篇 |
1991年 | 88篇 |
1990年 | 74篇 |
1989年 | 47篇 |
1988年 | 41篇 |
1987年 | 33篇 |
1986年 | 36篇 |
1985年 | 41篇 |
1984年 | 49篇 |
1983年 | 33篇 |
1982年 | 36篇 |
1981年 | 32篇 |
1980年 | 33篇 |
1979年 | 23篇 |
1978年 | 13篇 |
1977年 | 12篇 |
1976年 | 12篇 |
1973年 | 8篇 |
排序方式: 共有8793条查询结果,搜索用时 15 毫秒
61.
62.
Interconversion reactions of cyclodextrin glycosyltransferase (CGTase) among cyclodextrin (CD) homologues were experimentally investigated using each CD as a substrate in an aqueous, two-phase-forming polymer solution of dextran and polyethylene glycol. Degradation rate of -CD was highest and that of -CD was lowest among -, - and -CD with Bacillus macerans CGTase. Degradation of each CD was accelerated with dextran, while decelerated with polyethylene glycol. 相似文献
63.
64.
Zhuang Jin Hua Lin Sathish Srinivasan Jerome C. Nwachukwu Nelson Bruno Patrick R. Griffin Kendall W. Nettles Theodore M. Kamenecka 《Bioorganic & medicinal chemistry letters》2017,27(2):347-353
Adverse effects of glucocorticoids could be limited by developing new compounds that selectively modulate anti-inflammatory activity of the glucocorticoid receptor (GR). We have synthesized a novel series of steroidal GR ligands, including potent agonists, partial agonists and antagonists with a wide range of effects on inhibiting secretion of interleukin-6. Some of these new ligands were designed to directly impact conformational stability of helix-12, in the GR ligand-binding domain (LBD). These compounds modulated GR activity and glucocorticoid-induced gene expression in a manner that was inversely correlated to the degree of inflammatory response. In contrast, compounds designed to directly modulate LBD epitopes outside helix-12, led to dissociated levels of GR-mediated gene expression and inflammatory response. Therefore, these new series of compounds and their derivatives will be useful to dissect the ligand-dependent features of GR signaling specificity. 相似文献
65.
Emilien Loeuillard Haquima El Mourabit Lin Lei Sara Lemoinne Chantal Housset Axelle Cadoret 《生物化学与生物物理学报:疾病的分子基础》2018,1864(12):3688-3696
Portal myofibroblasts (PMF) form a sub-population of highly proliferative and proangiogenic liver myofibroblasts that derive from portal mesenchymal progenitors. Endoplasmic reticulum (ER) stress was previously shown to modulate fibrogenesis, notably in the liver. Our aim was to determine if ER stress occurred in PMF and affected their functions. PMF were obtained after their expansion in vivo from bile duct-ligated (BDL) rats and referred to as BDL PMF. Compared to standard PMF obtained from normal rats, BDL PMF were more myofibroblastic, as assessed by higher alpha-smooth muscle actin expression and collagen 1 production. Their proangiogenic properties were also higher, whereas their proliferative and migratory capacities were lower. CHOP expression was detected in the liver of BDL rats, at the leading edge of portal fibrosis where PMF accumulate. BDL PMF displayed ER dilatation and an overexpression of the PERK pathway downstream targets, Chop, Gadd34 and Trb3, in comparison with standard PMF. In vitro, the induction of ER stress by tunicamycin in standard PMF, caused a decrease in their proliferative and migratory activity, and an increase in their proangiogenic activity, without affecting their myofibroblastic differentiation. Conversely, the treatment of BDL PMF with the PERK inhibitor GSK2656157 reduced ER stress, which caused a decrease in their angiogenic properties, and restored their proliferative and migratory capacity. In conclusion, PMF develop ER stress as they expand with the progression of fibrosis, which further increases their proangiogenic activity, but also inhibits their proliferation and migration. This phenotypic switch may restrict PMF expansion while they support angiogenesis. 相似文献
66.
67.
68.
S. L. Hsieh W. L. Liaoand C. M. Kuo 《Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology》2001,130(4)
Stearoyl-CoA desaturase (EC 1.14.99.5) is a key enzyme in the biosynthesis of polyunsaturated fatty acids and the maintenance of the homeoviscous fluidity of biological membranes. The stearoyl-CoA desaturase cDNA in milkfish (Chanos chanos) was cloned by RT-PCR and RACE, and it was compared with the stearoyl-CoA desaturase in cold-tolerant teleosts, common carp and grass carp. Nucleotide sequence analysis revealed that the cDNA clone has a 972-bp open reading frame encoding 323 amino acid residues. Alignments of the deduced amino acid sequence showed that the milkfish stearoyl-CoA desaturase shares 79% and 75% identity with common carp and grass carp, and 63%–64% with other vertebrates such as sheep, hamsters, rats, mice, and humans. Like common carp and grass carp, the deduced amino acid sequence in milkfish well conserves three histidine cluster motifs (one HXXXXH and two HXXHH) that are essential for catalysis of stearoyl-CoA desaturase activity. However, RT-PCR analysis showed that stearoyl-CoA desaturase expression in milkfish is detected in the tissues of liver, muscle, kidney, brain, and gill, and more expression sites were found in milkfish than in common carp and grass carp. Phylogenic relationships among the deduced stearoyl-CoA desaturase amino acid sequence in milkfish and those in other vertebrates showed that the milkfish stearoyl-CoA desaturase amino acid sequence is phylogenetically closer to those of common carp and grass carp than to other higher vertebrates. 相似文献
69.
N. Sewald W. Hollweck K. Mütze C. Schierlinger L. C. Seymour K. Gaa K. Burger B. Koksch H. D. Jakubke 《Amino acids》1995,8(2):187-194
Summary Methodology for the synthesis and incorporation of-trifluoromethyl substituted amino acids into N- and C-terminal position of peptides is described. The incorporation of-trifluoromethyl substituted amino acids into strategical positions of peptides enhances proteolytic stability and lipophilicity. Furthermore, it improves transport rates in vivo and permeability through certain body barriers. 相似文献
70.
一类一级饱和反应系统的极限环 总被引:4,自引:0,他引:4
本文研究生化反应中一类饱和反应的数学模型:应用微分方程定性理论,完整地解决了该系统极限环的存在性、唯一性和不存在性等问题. 相似文献