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11.
12.
Gradient-tailored excitation for single-quantum NMR spectroscopy of aqueous solutions 总被引:76,自引:6,他引:70
Summary A novel approach to tailored selective excitation for the measurement of NMR spectra in non-deuterated aqueous solutions (WATERGATE, WATER suppression by GraAdient-Tailored Excitation) is described. The gradient echo sequence, which effectively combines one selective 180° radiofrequency pulse and two field gradient pulses, achieves highly selective and effective water suppression. This technique is ideally suited for the rapid collection of multi-dimensional data since a single-scan acquisition produces a pure phase NMR spectrum with a perfectly flat baseline, at the highest possible sensitivity. Application to the fast measurement of 2D NOE data of a 2.2. mM solution of a double-stranded DNA fragment in 90% H2O at 5 °C is presented. 相似文献
13.
Gary M. Graham Ludovico Guarini Thomas A. Moulton Subashree Datta Soldano Ferrone Patrizio Giacomini Robert S. Kerbel Paul B. Fisher 《Cancer immunology, immunotherapy : CII》1991,32(6):382-390
Summary Administration of interferon as a single therapeutic regimen in cancer patients with various neoplasias has had only limited efficacy in ameliorating the negative clinical course of their disease. In the present study, we have evaluated the effect of recombinant human fibroblast (IFN) and immune (IFN) interferon, alone and in combination, on growth, differentiation and the expression of class I and II histocompatibility locus antigens (HLA) and melanoma-associated antigens on the human melanoma cell line H0-1. The effect of combinations of interferons on the antigenic profile of human melanoma cells displaying different organ colonization and spontaneous metastatic potential in athymic nude mice was also determined. H0-1 cells were more sensitive to the antiproliferative activity of IFN than to IFN and the combination of interferons resulted in a potentiation of growth suppression. The antiproliferative effect of both interferons was greater in later-passage than in earlier-passage H0-1 cells, possibly reflecting alterations in the evolving tumor cell population as a result of long-term in vitro propagation and/or the selective outgrowth of cells with an increased growth rate. The enhanced growth suppression observed in H0-1 cells treated with the combination of IFN plus IFN was not associated with a significant increase in the level of melanin, a marker of melanoma differentiation, above that observed with either interferon used alone. IFN and IFN differentially modulated the expression of class I and II HLA and melanoma-associated antigens in H0-1 cells and a series of melanoma cells with different organ colonization and metastatic potential, including MeWo, MeM 50-10, MeM 50-17, 3S5 and 70W. No consistent potentiation or antagonism in the expression of any specific antigen was observed in any of the melanoma cell lines exposed to the combination of interferons. The present study demonstrates that the combination of IFN plus IFN can potentiate growth suppression in H0-1 human melanoma cells and that this effect is not associated with an increase in differentiation or a potentiation in antigenic modulation. In addition, no direct correlation between the expression of any specific antigen or its modulation by IFN or IFN, alone or in combination, and organ colonization and metastatic potential in nude mice was observed in the different melanoma cell lines. 相似文献
14.
Self-thinning in alfalfa, a dynamic process involving the progressive elimination of the weakest plants, was enhanced by Meloidogyne hapla. Alfalfa stand densities decreased exponentially with time and were reduced 62% (P = 0.05) in the presence of M. hapla. As stand densities decreased over time, mean plant weights increased at a rate 2.59 times faster in the absence of M. hapla. In a stepwise multiple regression analysis, 65% of the total variation in yield could be explained by changes in stand density and 85% by average weight of individual stems. Alfalfa yields were suppressed (P = 0.05) by M. hapla, with suppression generally increasing with time and as the nematode population density increased. Yield suppression was attributable primarily to the decline in plant numbers and to suppression in individual plant weights. 相似文献
15.
W. L. C. Vaz D. Hallmann R. M. Clegg A. Gambacorta M. De Rosa 《European biophysics journal : EBJ》1985,12(1):19-24
We have used the fluorescence recovery after photobleaching technique to study the translational diffusion, in L phase multibilayers of 1-palmitoyl-2-oleoylphosphatidylcholine (POPC), of fluorescent derivatives of 1-palmitoyl-2-oleoylphosphatidylethanolamine (NBD-POPE) and a membrane-spanning phosphatidylethanolamine (NBD-MSPE). The latter derivative was prepared from a membrane-spanning glycerol-dialkyl-glycerol tetraether lipid isolated from the thermophilic and acidophilic archaebacterium Sulfolobus solfataricus. The translational diffusion was examined between about 15° and 45°C. It is shown that over this temperature range the translational diffusion coefficient for NBD-MSPE is 2/3 that for NBD-POPE which spans only one monolayer of the bilayer. The result is interpreted in terms of existing models for translational diffusion in lipid membranes.Abbreviations D
t
translational diffusion coefficient
- FRAP
fluorescence recovery after photobleaching
- MSPE
a membrane-spanning phosphatidylethanolamine derived from a glycerol-dialkyl-glycerol tetraether lipid isolated from Sulfolobus solfataricus
- NBD
4-nitrobenz-2-oxa-1,3-diazolyl
- PE
phosphatidylethanolamine
- POPC
1-palmitoyl-2-oleoylphosphatidylcholine
- POPE
1-palmitoyl-2-oleoylphosphatidylethanolamine 相似文献
16.
17.
Barbara A. Israel Warren I. Schaeffer 《In vitro cellular & developmental biology. Plant》1987,23(9):627-632
Summary Using both normal and transformed rat liver epithelial cells to prepare cytoplasmic hybrids (cybrids) we have found evidence
to support the theory that the cytoplasm from a normal cell can suppress tumorigenicity. A unique aspect of this study is
that all of the cells utilized, both normal and malignantly transformed, were derived from an original cloned cell. We found
that fusing cytoplasts from normal cells to malignantly transformed whole cells resulted in cybrid clones which, when injected
into newborn rat pups, isogenic with those from which the cell culture was initiated, yielted tumors in 51% of the animals
injected compared to 92% of the animals injected with the tumorigenic parent. Those animals that did develop tumors from the
cybrid cells survived longer than those injected with cells from the tumorigenic parent. Thus, the cybrid, formed of cytoplasm
from both parents, was less tumorigenic than the malignantly transformed parent cell. When reconstituted cells were prepared
by fusing cytoplasts from normal cells with karyoplasts from malignantly transformed cells, a situation in which essentially
all of the cytoplasm of the reconstituted cell is derived from normal cells, the tumorigenic phenotype was extinguished.
This work was supported in part by United States Public Health Service grant CA12056, and grant CA09100 from the National
Cancer Institute, Bethesda, MD. This work is partial fulfillment for the degree of Doctor of Philosophy for B.A.I. 相似文献
18.
19.
Transforming growth factor-beta (TGF beta) is a growth modulator that stimulates the growth of fibroblastic cells but inhibits the growth of cells of epithelial origin. TGF beta also influences the production of extracellular matrix proteins, and of proteases and the type 1 plasminogen activator inhibitor (PAI-1) by cultured cells. TGF beta appears also to have various immunoregulatory effects, suppressing both T- and B-cell activities. It has been proposed that it might increase the expression of interleukin-1 (IL-1) mRNA in cultured human monocytes, thus potentiating immune functions. To analyze the role of TGF beta in IL-1 production we have now quantitated the effect of this factor on the production of biologically active IL-1 as well as IL-1 beta mRNA expression. The effect of TGF beta on IL-1 production optimally activated with bacterial lipopolysaccharide (LPS) was also studied. It was found that IL-1 activity and mRNA levels were rapidly elevated by LPS but not by TGF beta. Culture fluids from monocytes treated with TGF beta alone or with TGF beta plus LPS inhibited the proliferation of the test thymocytes. After gel filtration, the media from TGF beta-treated cultures showed no activity in the molecular weight area of IL-1 (approx. 15 kD), while the supernatants from TGF beta plus LPS-induced cells contained IL-1 activity in these fractions, the magnitude of which was, however, at the same level as in the culture fluids derived from cells stimulated with LPS alone.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
20.
Structural gene isolation and prepeptide sequence of gallidermin, a new lanthionine containing antibiotic 总被引:18,自引:0,他引:18
Norbert Schnell Karl-Dieter Entian Friedrich Götz Thomas Hörner Roland Kellner Günther Jung 《FEMS microbiology letters》1989,58(2-3):263-267
Peptide antibiotics containing lanthionine and 3-methyllanthionine bridges, named lantibiotics are of increasing interest. A new lantibiotic, gallidermin, has been isolated from Staphyloccus gallinarum. Here we report the isolation of its structural gene which we name gdmA. In all lantibiotics so far studied genetically, three peptides can be formally distinguished: (i) the primary translation product, which we call the prepeptide; (ii) the propeptide lacking the leader sequence and (iii) the mature lantibiotic. Unlike the plasmid-coded epidermin, gdmA is located on the chromosome. The gdmA locus codes for a 52 amino acid residue prepeptide, consisting of an alpha-helical leader sequence of hydrophilic character, which is separated from the C-terminus (propeptide) by a characteristic proteolytic processing site (Pro-2 Arg-1 Ile1). Although pro-gallidermin differs from pro-epidermin (a recently isolated lantibiotic) only by a single amino acid residue exchange. Leu instead of Ile, the N-terminus of the prepeptide differs by an additional two exchanges. 相似文献